Quantitative assessment of microenvironment characteristics and metabolic activity in glioma via multiphoton microscopy

Abstract

Tumor microenvironment and metabolic activity in gliomas are the important biomarkers to evaluate the progression of gliomas. Many evidences have suggested that the targeting of metabolic activity and tumor microenvironment simultaneously can be more effective to take the tumor therapy. Therefore, the noninvasive, accurate assessment of tumor microenvironment and metabolic activity is quite important in clinical practice. Multiphoton microscopy (MPM), based on two‐photon‐excited fluorescence and second harmonic generation was performed on unstained glioma tissues. With our combined image analysis approaches, our research findings indicate that MPM is able to qualitatively and quantitatively describe the microenvironment characteristics in gliomas, such as collage deposition in extracellular matrix, lymphocyte infiltration and tumor angiogenesis, etc. Meanwhile, the metabolic activity can also be quantitatively evaluated by optical redox ratio, NADH and FAD intensity. With the microendoscope and fiberscope are portable, MPM technique can be used to perform in‐vivo studies and clinical examinations in gliomas.

Automatic and label-free detection of meningioma in dura mater using the combination of multiphoton microscopy and image analysis

“…Previous reports have shown increased NADH content in tumors 32 , 33 . Our previous studies on normal and glioma human brain tissues also demonstrated higher NADH levels in gliomas 34 . In this study, when the dura mater was invaded by tumor cells, the metabolic activity increased, which explains the higher NADH content in the meningioma-infiltrated dura mater.…”

Label-free imaging of human brain tissue at subcellular resolution for potential rapid intra-operative assessment of glioma surgery

“…Previous studies have demonstrated that that multiphoton excitation of autofluorescence hold the potential to distinguish tumor tissue and normal brain based on the intensity and lifetime of fluorescence 10 , 11 . Some microenvironment characteristics in gliomas, such as collagen deposition in extracellular matrix and tumor angiogenesis, were identified in the MPM images for the brain slices after frozen cutting previously 14 , 15 . We found that MPM provides structural information similar to that of the co-located histopathology for fresh brain tissues.…”

“…To improve intraoperative assessment, various optical techniques have been developed for rapid, high-resolution imaging of fresh human brain tissues, including microscopy with ultraviolet surface excitation (MUSE) 5 , light-sheet microscopy (LSM) 6 , optical coherence tomography (OCT) 7 , stimulated Raman scattering (SRS) microscopy 8 , 9 , and multiphoton microscopy (MPM) 10 - 15 . For MUSE and LSM, fluorescent dyes are required and tissue must be rinsed prior to imaging.…”

Diagnosis of Glioma Molecular Markers by Terahertz Technologies

“…Several algorithms for 2D segmentation have been proposed: The Otsu thresholding procedure to maximize the intra-class variance of segmented areas along with mathematical morphology to filter noise and unreliable data, as well as Fuzzy C-means and graph cuts in LAB colorspace [199].…”