Quantitative assessment of melanomacrophage centres (MMCs) in dab Limanda limanda along a pollution transect in the German Bight

Bucke, D.; Vethaak, A.D.; Lang, Thomas

doi:10.3354/meps091193

Cited by 35 publications

(24 citation statements)

References 13 publications

(13 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Decreasing MA activity, which was found to be correlated with residues of dieldrin in male flounder liver, was in most cases accompanied by a small macrophage aggregate area (Broeg, unpublished data). This is in agreement with studies of Bucke et al (1992) on dab spleen macrophages. In contrast, other studies showed contradicting results and found an increase in macrophage aggregate numbers in spleen and kidney following exposure to sewage sludge (Secombes et al 1991).…”

Section: Macrophage Activity Testsupporting

confidence: 93%

The use of fish metabolic, pathological and parasitological indices in pollution monitoring

Broeg¹,

Zander

Diamant

et al. 1999

Helgoland Marine Research

View full text Add to dashboard Cite

An integrated biological effect monitoring concept has been tested in flounder (Platichthys flesus L.) from four locations with different anthropogenic impact in the German Bight. During 3 years of sampling, biomarkers at all levels of biological organisation from the molecular to the ecosystem level were applied and tested on 742 individual fish of body lengths between 18 and 25 cm. At the ecosystem level, the fish were taken as a habitat for the parasite assemblage. The hypothesis was that changes in the environment might lead to changes in the species diversity of parasites and in the infection intensity of single species, as well as between heteroxenic and monoxenic parasite species (H/M ratio). At the molecular level, activity of the CYP1A-dependent monooxygenase ethoxyresorufin O-deethylase (EROD) was used as a biomarker of exposure. At the subcellular level, the integrity of lysosomal membranes in hepatocytes was taken as an indicator of non-specific acute and chronic toxic effects. Both biomarkers are recommended by the ICES Advisory Committee on the Marine Environment for the application in biological effects monitoring programmes. In addition, neutral lipid content in the liver was used as a marker for pathologically induced fat accumulation. In the same individual fish, a new method for the measurement of macrophage aggregate activity in the liver was tested for its application and reliability in reflecting immunosuppression. Tests were accompanied by chemical analysis of standard organochlorine and heavy metal residues in flounder tissue. A total of 33 parasite species were found. As an indicator species, the mean abundance of Trichodina sp. reflected best the pollution gradient observed with highest infection intensity at the most polluted location. Species diversity was significantly higher in fish caught near the reference site and significantly lower in fish from the polluted Elbe estuary. The use of the heteroxenous/monoxenous species ratio as a marker was not useful at the locations investigated because of the dominance of heteroxenous species at all habitats. Since EROD activity and macrophage aggregate activity were dependent on sex and maturity of female flounder, only male fish were taken into consideration for the integrated evaluation of data. All biochemical and histochemical tests were able to reflect accurately the site-specific differences, as well as an observed pollution event at the end of 1995 as determined by chemical analyses. The correlation analysis revealed a connection not only between the single parasitological and biochemical parameters but also within these groups. The non-specific immune response and Trichodina infection intensity were correlated with all other parameters, leading to the assumption that these may serve as links between the lowest and the highest levels of biological organisation. The simultaneous use of metabolic and parasitological results facilitated the interpretation of the observed variations of the data and the distinction between natural ...

show abstract

Section: Macrophage Activity Testsupporting

confidence: 93%

The use of fish metabolic, pathological and parasitological indices in pollution monitoring

Broeg¹,

Zander

Diamant

et al. 1999

Helgoland Marine Research

View full text Add to dashboard Cite

show abstract

“…Kranz (1989) quantified splenic hemosiderin in the dab L. limanda, which was more commonly found in fish with ulcerous livers than in fish that had recovered from the ulcer disease. Also in the dab, Bucke et al (1992) found significantly reduced hemosiderin content at sites with the highest and the lowest levels of contaminated sediment; however, these authors recognized that the fish may have migrated between sites. Manual counting of MAs is potentially ambiguous in salmonids, depending on the definition of a MA, and is clearly time-consuming.…”

Section: Discussionmentioning

confidence: 90%

“…Splenic hemosiderin was quantified by Kranz (1989), Bucke et al (1992) and Khan & Nag (1993), and Mizuno et al (2002) quantified melanin in the kidney interstitium in masu salmon Oncorhynchus masou.…”

Section: Introductionmentioning

confidence: 99%

Quantitative evaluation of macrophage aggregates in brook trout Salvelinus fontinalis and rainbow trout Oncorhynchus mykiss

Schwindt¹,

Truelove²,

Schreck³

et al. 2006

Dis. Aquat. Org.

View full text Add to dashboard Cite

Macrophage aggregates (MAs) occur in various organs of fishes, especially the kidney, liver and spleen, and contain melanin, ceroid/lipofuscin and hemosiderin pigments. They have been used as indicators of a number of natural and anthropogenic stressors. Macrophage aggregates occur in salmonids but are poorly organized, irregularly shaped, and are generally smaller than those in derived teleosts. These features complicate quantification, and thus these fishes have seldom been used in studies correlating MAs with environmental stressors. To alleviate these complications, we developed color filtering algorithms for use with the software package ImagePro Plus ® (Media Cybernetics) that select and quantify pigmented area (i.e. colors ranging from gold to brown to black) in tissue sections. Image analysis results compared well with subjective scoring when tested on brook trout Salvelinus fontinalis and rainbow trout Oncorhynchus mykiss captured from high-elevation lakes or hatcheries. Macrophage aggregate pigments correlated positively with age and negatively with condition factor. Within individual fish, pigmentation correlated positively among organs, suggesting that the kidney, liver or spleen are suitable indicator organs. In age-matched fishes, MA pigments were not different between hatcheries and lakes in the organs examined. Between lakes, differences in pigments were observed in the kidney and spleen, but were not explained by age, condition factor, sex or maturation state. Our results indicate that quantification of the area occupied by MA pigments is an efficient and accurate means of evaluating MAs in salmonid organs and that organ pigmentation correlates with age and condition factor, as seen in studies with more derived fishes.

show abstract

“…These results support the view proposed by other studies that hemosiderin concentration can represent a useful tool for monitoring fish health (Bucke at at. 1984(Bucke at at. .…”

Section: A-collection Offish Samplesmentioning

confidence: 99%

Heavy Lmetals Accumulation and Macronutrients in the Livers of Some Fish Species of Bardawil Lagoon and Their Histological Changes

Yacoub

Satar

2003

Egypt. J. of Aquatic Biolo. and Fish.

View full text Add to dashboard Cite

Quantitative assessment of melanomacrophage centres (MMCs) in dab Limanda limanda along a pollution transect in the German Bight

Cited by 35 publications

References 13 publications

The use of fish metabolic, pathological and parasitological indices in pollution monitoring

The use of fish metabolic, pathological and parasitological indices in pollution monitoring

Quantitative evaluation of macrophage aggregates in brook trout Salvelinus fontinalis and rainbow trout Oncorhynchus mykiss

Heavy Lmetals Accumulation and Macronutrients in the Livers of Some Fish Species of Bardawil Lagoon and Their Histological Changes

Contact Info

Product

Resources

About