Quantitative assessment of ischemic tissue damage in ovarian cortical tissue with or without antioxidant (ascorbic acid) treatment

Kim, S.Samuel; Yang, Hee Jung; Kang, Hee-Gyoo; Lee, Hang Heun; Lee, Hoi Chang; Ko, Duck Sung; Gosden, Roger G.

doi:10.1016/j.fertnstert.2004.05.022

Cited by 152 publications

(89 citation statements)

References 15 publications

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“…Previously, we have shown that oocytes survive in adequate numbers in subcutaneously transplanted cryopreserved ovarian tissue [17]. Granulosa cells are reportedly more vulnerable to ischemia than primordial follicles [31], and in this study, we demonstrated a higher than normal rate of apoptosis in granulosa cells from cryopreserved ovaries (Fig. 4).…”

Section: Discussionsupporting

confidence: 72%

“…The loss of primordial follicles after grafting has been attributed mainly to ischemia and hypoxia before the grafted fragment is revascularized [17,31,35]. In this study, we have demonstrated that granulosa cell-oocyte interaction is damaged in tissue that has undergone cryopreservation and subcutaneous transplantation.…”

Section: Discussionmentioning

confidence: 68%

“…4). Primordial follicles, which are relatively dormant, tolerate freezing and thawing better than more developed follicles compared to other stages [31].…”

Section: Discussionmentioning

confidence: 95%

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Abnormally low expression of connexin 37 and connexin 43 in subcutaneously transplanted cryopreserved mouse ovarian tissue

Lee

et al. 2008

J Assist Reprod Genet

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Purpose To analyze the gap junction proteins connexin 37 (Cx37) and connexin 43 (Cx43) after subcutaneous transplantation of cryopreserved mouse ovarian tissue. Methods Expression of gap junction genes was assessed by immunohistochemistry and real-time polymerase chain reaction (PCR) in transplanted cryopreserved ovarian tissue compared with that of normal ovarian tissue. Apoptosis of ovarian cells was evaluated by using the terminal deoxynucleotidyl transferase-mediated biotinylated deoxyuridine triphosphates nick end-labeling method.Results After subcutaneous transplantation, Cx37 and Cx43 mRNA and protein expression were significantly lower in cryopreserved than in normal ovarian tissue. Apoptosis was increased in granulosa cells from antral follicles of the cryopreserved tissue. Conclusion After cryopreservation and subcutaneous transplantation of ovarian tissue, proteins forming gap junctions between oocytes and granulosa cells are under-expressed compared with normal controls.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 68%

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Abnormally low expression of connexin 37 and connexin 43 in subcutaneously transplanted cryopreserved mouse ovarian tissue

Lee

et al. 2008

J Assist Reprod Genet

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“…It has been reported that functional vessels within the graft were detected by both magnetic resonance imaging and histological exam from day 7 onwards when rat ovaries were transplanted into the muscles of castrated nude mice [30]. Kim et al, [31] showed that the ovarian tissue could tolerate ischaemia for at least 2 h at 0°C or at room temperature, and that a water soluble antioxidant (ascorbic acid) reduces apoptosis in ovarian cortex by up to 24 h in the case of incubation in vitro. It has been reported that treatment with vitamin E, a lipid soluble antioxidant, improved the survival of follicles in ovarian grafts by reducing ischemic injury [32].…”

Section: Discussionmentioning

confidence: 99%

Beneficial effect of desialylated erythropoietin administration on the frozen-thawed canine ovarian xenotransplantation

Suzuki

Ishijima

Maruyama

et al. 2008

J Assist Reprod Genet

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Purpose The main drawback of ovarian cryopreservation followed by transplantation is that a large proportion of follicles are lost after transplantation. Thus, effects of erythropoietin (EPO) and desialylated EPO administration on the frozen-thawed canine ovarian xenotransplantation were examined. Methods The protective and survival-promoting effects of EPO and desialylated EPO on the follicles of frozen-thawed canine ovaries after transplantation were examined using NOD-SCID mice. Frozen-thawed dog ovarian tissue with 400 U/kg of EPO or asialo EPO was placed into the ovarian bursa.Results At 4 weeks after the transplantation, the ovaries were removed and subjected to histological examination. The survival rate of early primary follicles was 15.2% in the EPO group and 157.6% in the asialo EPO group, in contrast to 10.1% in the untreated group. Conclusions These results demonstrate that administration of asialo EPO could be effectively used to enhance the survival of the follicles of transplanted cryopreserved ovaries.

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“…[27][28][29][30][31][32][33][34][35][36][37][38] Moreover, most of these studies did not take the viability of the stromal cell compartment into account. [27][28][29][30][31][32][33][34][35][36][37][38] This compartment, however, is essential for post-autotransplantation neovascularization, follicle survival, and life span of the ovarian graft [39] and is considered to be more sensitive to ischemic and cryoinjury than primordial follicles [8,40].…”

Section: Introductionmentioning

confidence: 99%

Efficacy of ovarian tissue cryopreservation in a major European center

Bastings

Liebenthron

Westphal

et al. 2014

J Assist Reprod Genet

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Quantitative assessment of ischemic tissue damage in ovarian cortical tissue with or without antioxidant (ascorbic acid) treatment

Cited by 152 publications

References 15 publications

Abnormally low expression of connexin 37 and connexin 43 in subcutaneously transplanted cryopreserved mouse ovarian tissue

Abnormally low expression of connexin 37 and connexin 43 in subcutaneously transplanted cryopreserved mouse ovarian tissue

Beneficial effect of desialylated erythropoietin administration on the frozen-thawed canine ovarian xenotransplantation

Efficacy of ovarian tissue cryopreservation in a major European center

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