Quantitative assessment of collagen I liquid crystal organizations: role of ionic force and acidic solvent, and evidence of new phases

Peixoto, Paulo De Sa; Deniset‐Besseau, Ariane; Schanne-Klein, Marie-Claire; Mosser, Gervaise

doi:10.1039/c1sm06076g

Cited by 27 publications

(37 citation statements)

References 37 publications

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“…After ice-templating ( Figure 2 . After ammonia treatment, small fibrils were visible displaying some degree of local organization, reminiscent of the previously-described liquid crystal organizations of collagen [28][29][30] (Figure 2 bottom middle). At the end of the process, after steps 3 and 4, the previously-observed dense organized areas of small fibrils (Supporting Information Figure S2A Figure S3A) as opposed to dipping freeze-casted scaffold directly after step 1 into a neutral buffer such as PBS 10X that resulted in occluded pores (Supporting Information Figure S3B).…”

Section: Topotactic Fibrillogenesis Of Ice-templated Collagen Scaffoldssupporting

confidence: 68%

Topotactic Fibrillogenesis of Freeze-Cast Microridged Collagen Scaffolds for 3D Cell Culture

Rieu

Parisi

Mosser

et al. 2019

ACS Appl. Mater. Interfaces

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Type I collagen is the main component of the extra-cellular matrix (ECM). In vitro, under a narrow window of physico-chemical conditions, type I collagen self-assembles to form complex supramolecular architectures reminiscent of those found in native ECM. Presently, a major challenge in collagen-based biomaterials is to couple the delicate collagen fibrillogenesis events with a controlled shaping process in non-denaturating conditions. In this work an ice-templating approach promoting the structuration of collagen into macroporous monoliths is used. Instead of common solvent removal procedures, a new topotactic conversion approach yielding self-assembled ordered fibrous materials is implemented. These collagen-only, non-cross-linked scaffolds exhibit uncommon mechanical properties in the wet state. With the help of the ice-patterned micro-ridge features, Normal Human Dermal Fibroblasts and C2C12 murine myoblasts successfully migrate and form highly-aligned populations within the resulting 3D biomimetic collagen scaffolds. These results open a new pathway to the development of new tissue engineering scaffolds ordered across various organization levels from the molecule to the macropore, and are of particular interest for biomedical applications where large scale 3D cell alignment is needed such as for muscular or nerve reconstruction.

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Section: Topotactic Fibrillogenesis Of Ice-templated Collagen Scaffoldssupporting

confidence: 68%

Topotactic Fibrillogenesis of Freeze-Cast Microridged Collagen Scaffolds for 3D Cell Culture

Rieu

Parisi

Mosser

et al. 2019

ACS Appl. Mater. Interfaces

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“…γ = 0.04 and k 24 = 0.5 on the coexistence line are chosen to recover R * C /R * L 4. Takingq = 4 µm −1 is consistent with in vitro studies of chiral nematic collagen solutions [30], and leads to radiiR C ≈ 106 nm and R L ≈ 27 nm.…”

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confidence: 69%

Phase-field collagen fibrils: Coupling chirality and density modulations

Cameron

Kreplak

Rutenberg

2020

Phys. Rev. Research

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To describe the interaction between longitudinal density modulations along collagen fibrils (the D-band) with the radial twist-field of molecular orientation (double-twist), we couple phase-fieldcrystal (PFC) with liquid-crystalline free-energies to obtain a hybrid model of equilibrium collagen fibril structure. We numerically compute the resulting axial and radial structure. We find two distinct fibrillar phases, 'L' and 'C', with a coexistence line that ends in an Ising-like critical point. We propose that coexistence between these phases can explain the bimodal distribution of fibril radii that has been widely reported within tendon tissues. Tensile strain applied to our model fibrils straightens the average fibrillar twist and flattens the D-band modulation. Our PFC approach should apply directly to other longitudinally-modulated chiral filaments, such as fibrin and intermediate filaments.

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“…Despite these fascinating properties, the practical use is limited because it is difficult to control the orientation of the plasmonic nanoparticles. LLC is found in condensed biomaterials such as DNA, [27,28] food dyes, [29] cellulose, [30,31] collagen, [32,33] and bacteriophage, [34,35] which are abundantly produced every year. Simple mechanical shearing results in long-range DNA-gold nanorod arrays which show parallel, perpendicular, and zigzag configurations due to the competition between the shear force and DNA elasticity.…”

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confidence: 99%

“…[26] Among the various types of host LC materials, it is well known that lyotropic LC (LLC) is the most promising candidate for controlling GNRs because LLC itself is dispersed in the solvent, making it easy to homogeneously mix with GNR. LLC is found in condensed biomaterials such as DNA, [27,28] food dyes, [29] cellulose, [30,31] collagen, [32,33] and bacteriophage, [34,35] which are abundantly produced every year. LLC is found in condensed biomaterials such as DNA, [27,28] food dyes, [29] cellulose, [30,31] collagen, [32,33] and bacteriophage, [34,35] which are abundantly produced every year.…”

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confidence: 99%

Highly Aligned Plasmonic Gold Nanorods in a DNA Matrix

Jeong

Kim

Yoon

2017

Adv Funct Materials

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Since the Lycurgus Cup was made in the 4th century, metal nanoparticles have attracted much interest due to the characteristics of the plasmonic and metamaterials that show beautiful colors. Despite these fascinating properties, the practical use is limited because it is difficult to control the orientation of the plasmonic nanoparticles. Here, highly aligned plasmonic gold nanorods are obtained using self-assembled DNA material. Simple mechanical shearing results in long-range DNA-gold nanorod arrays which show parallel, perpendicular, and zigzag configurations due to the competition between the shear force and DNA elasticity. The resulting surface plasmonic resonance properties of the aligned DNA-gold nanorods film show highly polarization-dependent behavior in a large area, which is critical for optical and photonic applications. This simple way to form anisotropic plasmonic films can be used for plasmonic nanoparticles in potential applications such as displays and sensors.The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/adfm.201703790.Recently, a few methods for controlling the GNRs based on the liquid crystal (LC) material used as a host medium were suggested to control the guest material-GNRs with external stimuli such as electric field, [21] magnetic field, [22] and mechanical force, [9] in which GNRs are spontaneously arranged with the LC director [9,21,[23][24][25] or trapped in the defect of LCs. [26] Among the various types of host LC materials, it is well known that lyotropic LC (LLC) is the most promising candidate for controlling GNRs because LLC itself is dispersed in the solvent, making it easy to homogeneously mix with GNR. Indeed, the phase transition occurs as a function of the solute concentration, here LLC material and GNR, meaning that various conformations of GNR can be achieved. LLC is found in condensed biomaterials such as DNA, [27,28] food dyes, [29] cellulose, [30,31] collagen, [32,33] and bacteriophage, [34,35] which are abundantly produced every year. Among these LLC materials, DNA extracted from plant or fish are easy to get and indeed very cheap compared with typical synthetic polymers and LC materials used in the practical applications. Particularly, DNA can be used as a building block to make small structures since DNA has the specific sequences that can be spontaneously assembled together to make the programmable structure such as origami. [36,37] Here, we fabricate well-oriented GNRs in DNA medium. Compared with other LLC, the orientation of DNA is easily controlled by applying simple mechanical shearing [38][39][40] (Table S1, Supporting Information). Thus, the DNA was selected as the medium to orient GNRs. The DNA material used in this study is salmon sperm DNA duplex (purchased from Sigma-Aldrich, St. Louis, MO, USA) with a Gaussian distribution in base pairs, for which the center point is ≈2000 base pairs and the corresponding length is ≈680 nm. [41] The DNA material in aqueous solution forms the LLC phase at t...

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Quantitative assessment of collagen I liquid crystal organizations: role of ionic force and acidic solvent, and evidence of new phases

Cited by 27 publications

References 37 publications

Topotactic Fibrillogenesis of Freeze-Cast Microridged Collagen Scaffolds for 3D Cell Culture

Topotactic Fibrillogenesis of Freeze-Cast Microridged Collagen Scaffolds for 3D Cell Culture

Phase-field collagen fibrils: Coupling chirality and density modulations

Highly Aligned Plasmonic Gold Nanorods in a DNA Matrix

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