Quantitative assessment of cerebral venous blood T<sub>2</sub> in mouse at 11.7T: Implementation, optimization, and age effect

Wei, Zhiliang; Xu, Jiadi; Liu, Peiying; Chen, Lin; Li, Wenbo; Zijl, Peter van; Lu, Hanzhang

doi:10.1002/mrm.27046

Cited by 16 publications

(24 citation statements)

References 56 publications

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“…Y v was assessed non-invasively from the confluence of sagittal sinuses with a T 2 -relaxation-under-spin-tagging (TRUST) technique, which was originally developed on human scanners and recently optimized for animal MRI systems (30)(31)(32). In order to visualize the confluence of sagittal sinuses, an axial time-of-flight (TOF) sequence was first performed with the following parameters: TR/TE = 20/2.6 ms, field of view (FOV) = 16 × 16 mm, matrix size = 256 × 256, 35 axial slices, slice thickness = 0.5 mm, and scan duration = 2.2 min.…”

Section: Cerebral Venous Oxygenation (Y V )mentioning

confidence: 99%

“…In order to visualize the confluence of sagittal sinuses, an axial time-of-flight (TOF) sequence was first performed with the following parameters: TR/TE = 20/2.6 ms, field of view (FOV) = 16 × 16 mm, matrix size = 256 × 256, 35 axial slices, slice thickness = 0.5 mm, and scan duration = 2.2 min. The TRUST scan was then positioned based on the TOF images (31), and was repeated three times to improve precision. The TRUST sequence used the following parameters: TR/TE = 3500/6.5 ms, FOV = 16 × 16 mm, matrix size = 128 × 128, slice thickness = 0.5 mm, EPI factor = 16, inversion-slab thickness = 2.5 mm, post-labeling delay = 1000 ms, eTE = 0.25, 20, 40 ms, echo spacing of eTE = 5.0 ms, and scan duration = 2.8 min.…”

Section: Cerebral Venous Oxygenation (Y V )mentioning

confidence: 99%

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Age-Related Alterations in Brain Perfusion, Venous Oxygenation, and Oxygen Metabolic Rate of Mice: A 17-Month Longitudinal MRI Study

et al. 2020

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Background: Characterization of physiological parameters of the aging brain, such as perfusion and brain metabolism, is important for understanding brain function and diseases. Aging studies on human brain have mostly been based on the cross-sectional design, while the few longitudinal studies used relatively short follow-up time compared to the lifespan. Objectives: To determine the longitudinal time courses of cerebral physiological parameters across the adult lifespan in mice. Methods: The present work examined longitudinal changes in cerebral blood flow (CBF), cerebral venous oxygenation (Y v), and cerebral metabolic rate of oxygen (CMRO 2) using MRI in healthy C57BL/6 mice from 3 to 20 months of age. Each mouse received 16 imaging sessions at an ∼1-month interval. Results: Significant increases with age were observed in CBF (p = 0.017) and CMRO 2 (p < 0.001). Meanwhile, Y v revealed a significant decrease (p = 0.002) with a non-linear pattern (p = 0.013). The rate of change was 0.87, 2.26, and −0.24% per month for CBF, CMRO2, and Y v , respectively. On the other hand, systemic parameters such as heart rate did not show a significant age dependence (p = 0.47). No white-matter-hyperintensities (WMH) were observed on the T 2-weighted image at any age of the mice. Conclusion: With age, the mouse brain revealed an increase in oxygen consumption. This observation is consistent with previous findings in humans using a cross-sectional design and suggests a degradation of the brain's energy production or utilization machinery. Cerebral perfusion remains relatively intact in aged mice, at least until 20 months of age, consistent with the absence of WMH in mice.

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Section: Cerebral Venous Oxygenation (Y V )mentioning

confidence: 99%

Section: Cerebral Venous Oxygenation (Y V )mentioning

confidence: 99%

Age-Related Alterations in Brain Perfusion, Venous Oxygenation, and Oxygen Metabolic Rate of Mice: A 17-Month Longitudinal MRI Study

et al. 2020

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“…Moreover, neurodegenerative diseases, such as Alzheimer’s disease and Parkinson’s disease, can be evaluated by CBF because of the presence of coupling between CBF and cerebral metabolism. Furthermore, measurement of CBF can serve as a building block of probing more advanced physiological parameters, such as cerebral metabolic rate of oxygen, by combining CBF information with oxygenation methods, for example, T 2 ‐relaxation‐under‐spin‐tagging . Additionally, CBF can be used to evaluate the effects of drug or treatment in molecular imaging or intervention studies.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, measurement of CBF can serve as a building block of probing more advanced physiological parameters, such as cerebral metabolic rate of oxygen, by combining CBF information with oxygenation methods, for example, T 2relaxation-under-spin-tagging. 23,24 Additionally, CBF can be used to evaluate the effects of drug or treatment in molecular imaging or intervention studies.…”

Section: F I G U R E 3 Summary Of a Peak And B Mean Bf Velocities Imentioning

confidence: 99%

Optimization of phase‐contrast MRI for the estimation of global cerebral blood flow of mice at 11.7T

Wei

Chen

Lin

et al. 2018

Magnetic Resonance in Med

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Purpose To optimize phase‐contrast (PC) MRI for the measurement of global cerebral blood flow (CBF) in the mouse at 11.7T. Methods We determined proper velocity encoding (VENC) for internal carotid arteries (ICAs) and vertebral arteries (VAs). Next, we optimized spatial resolution of the sequence. To shorten scan time without compromising data quality, we further optimized repetition time and developed a reduced field‐of‐view (FOV) scheme for ICA and VA PC MRI. Whole‐brain volume was determined with T2‐weighted image to obtain unit‐volume CBF. Results Peak flow velocities were 13.8 ± 1.7, 14.4 ± 0.6, 6.5 ± 1.7, and 6.7 ± 1.3 cm/s for left ICA, right ICA, left VA, and right VA, respectively. Thus, VENC values of 20 and 10 cm/s were chosen for ICA and VA PC MRI, respectively. An in‐plane spatial resolution of 50 × 50 μm2 was found to provide a reasonable trade‐off between reducing partial‐volume effects and maintaining signal‐to‐noise ratio. Because of the fact that saturated spins in the imaging slice are rapidly replaced by fresh spins, TR of the sequence can be decreased to as short as 15 ms without reducing signal intensity, thereby substantially lowering scan time. Moreover, reduced FOV along the phase‐encoding direction was able to shorten scan time by 33.3% while maintaining measurement accuracy. With these optimizations, it took 96 seconds to evaluate CBF with a test‐retest variability of approximately 5% and an inter‐rater correlation of >0.95. Global unit‐volume CBF was found to be 279.5 ± 11.1 mL of blood/100 ml of tissue/min. Conclusion We have optimized PC MRI for noninvasive quantification of blood flow in mice at 11.7T.

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“…Therefore, in this study, we utilized noncontrast‐agent T 2 ‐relaxation‐under‐spin‐tagging (TRUST) 16,17 and phase‐contrast (PC) 18,19 MRI techniques to investigate OEF, CBF and CMRO 2 in a dynamic fashion during the first 3 hours after CA and the ROSC in a rat model. To quantitatively evaluate neurologic recovery, a validated neurologic deficit score (NDS) 20 was determined at 24 hours after the ROSC.…”

Section: Introductionmentioning

confidence: 99%

Acute‐stage MRI cerebral oxygen consumption biomarkers predict 24‐hour neurological outcome in a rat cardiac arrest model

et al. 2020

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Brain injury following cardiac arrest (CA) is thought to be caused by a sudden loss of blood flow resulting in disruption in oxygen delivery, neural function and metabolism. However, temporal trajectories of the brain's physiology in the first few hours following CA have not been fully characterized. Furthermore, the extent to which these early measures can predict future neurological outcomes has not been determined. The present study sought to perform dynamic measurements of cerebral blood flow (CBF), oxygen extraction fraction (OEF) and cerebral metabolic rate of oxygen (CMRO 2) with MRI in the first 3 hours following the return of spontaneous circulation (ROSC) in a rat CA model. It was found that CBF, OEF and CMRO 2 all revealed a time-dependent increase during the first 3 hours after the ROSC. Furthermore, the temporal trajectories of CBF and CMRO 2 , but not OEF, were different across rats and related to neurologic outcomes at a later time (24 hours after the ROSC) (P < .001). Rats who manifested better outcomes revealed faster increases in CBF and CMRO 2 during the acute stage. When investigating physiological parameters measured at a single time point, CBF (ρ = 0.82, P = .004) and CMRO 2 (ρ = 0.80, P = .006) measured at $ 3 hours post-ROSC were positively associated with neurologic outcome scores at 24 hours. These findings shed light on brain physiological changes following CA, and suggest that MRI measures of brain perfusion and metabolism may provide a potential biomarker to guide post-CA management. K E Y W O R D S cardiac arrest, cerebral blood flow, cerebral metabolic rate of oxygen, MRI, neurologic deficit score, oxygen consumption, phase contrast, TRUST 1 | INTRODUCTION Cardiac arrest (CA) carries a low survival rate ($10%) and unfavorable outcomes. 1,2 Poor functional outcomes and mortality in CA are primarily driven by brain injury that occurs from a sudden loss of blood supply to the brain. The lack of blood flow leads to rapid changes in intracellular and extracellular ion concentrations with the development of intracellular acidosis. Furthermore, the failure of maintaining ionic balance results in membrane disruption, irreversible neuronal injury and, eventually, cell death. 3 As a critical management strategy for CA, effective cardiopulmonary

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Quantitative assessment of cerebral venous blood T₂ in mouse at 11.7T: Implementation, optimization, and age effect

Cited by 16 publications

References 56 publications

Age-Related Alterations in Brain Perfusion, Venous Oxygenation, and Oxygen Metabolic Rate of Mice: A 17-Month Longitudinal MRI Study

Age-Related Alterations in Brain Perfusion, Venous Oxygenation, and Oxygen Metabolic Rate of Mice: A 17-Month Longitudinal MRI Study

Optimization of phase‐contrast MRI for the estimation of global cerebral blood flow of mice at 11.7T

Acute‐stage MRI cerebral oxygen consumption biomarkers predict 24‐hour neurological outcome in a rat cardiac arrest model

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Quantitative assessment of cerebral venous blood T2 in mouse at 11.7T: Implementation, optimization, and age effect

Cited by 16 publications

References 56 publications

Age-Related Alterations in Brain Perfusion, Venous Oxygenation, and Oxygen Metabolic Rate of Mice: A 17-Month Longitudinal MRI Study

Age-Related Alterations in Brain Perfusion, Venous Oxygenation, and Oxygen Metabolic Rate of Mice: A 17-Month Longitudinal MRI Study

Optimization of phase‐contrast MRI for the estimation of global cerebral blood flow of mice at 11.7T

Acute‐stage MRI cerebral oxygen consumption biomarkers predict 24‐hour neurological outcome in a rat cardiac arrest model

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Quantitative assessment of cerebral venous blood T₂ in mouse at 11.7T: Implementation, optimization, and age effect