Quantitative assessment of angiogenesis in the chick embryo and its chorioallantoic membrane by computerised analysis of angiographic images

Nikiforidis, George; Papazafiropoulos, D.; Siablis, Dimitrios; Karnabatidis, Dimitrios; Hatjikondi, O.; Dimopoulos, J

doi:10.1016/s0720-048x(98)00025-4

Cited by 29 publications

(21 citation statements)

References 26 publications

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“…The concept of angiogenesis based therapy is well advocated in the literature and the development of both suppressors and inhibitors of angiogenesis has been proposed for the treatment of genitourinary malignancies [1][2][3][4][5][6][7][8][9][10][11].…”

Section: Discussionmentioning

confidence: 99%

“…Angiographic images where digitized and subsequently processed as previously described in detail (Figure 1a-e) [2,5,7]. The vessels were classified into two categories according to their diameter (smaller or greater than 200 lm), and thus the effect of irradiation was observed both in big and small vessels.…”

Section: Methodsmentioning

confidence: 99%

“…In vitro models have shown that endothelial cells have moderate radiosensitivity compared to other cell types such as fibroblasts, and that survival is dose-, time-and cell cycle-dependent. In vivo models have reproduced similar results with regard to vascular response to irradiation, providing more accurate representations of angiogenic activity; cells are irradiated within their natural environment, thus achieving protection of the endothelial layers and potentially inducing repair of irradiation damage [1][2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

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Digital subtraction angiography and computer assisted image analysis for the evaluation of the antiangiogenetic effect of ionizing radiation on tumor angiogenesis

et al. 2006

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Digital subtraction angiography and computer assisted image analysis for the evaluation of the antiangiogenetic effect of ionizing radiation on tumor angiogenesis

et al. 2006

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“…The latter tools provide image processing techniques, which allow measurements of vascular length after being applied manually on the examined angiographic images. Additional work in literature ( [9]- [12]) presents computer-aided methods for angiogenesis quantification which involve manual processing of the images for calculating vessel density, vessel length and branching points. To our knowledge there is no automated software for angiogenesis quantification currently available.…”

Section: Related Workmentioning

confidence: 99%

“…CAM is a highly vascular structure lining the inner surface of the egg shell. The study of the formation of its vessels, at different stages of development, is efficiently indicative for the phenomenon ( [7]- [12]). The aim of this study was to test and develop an automated image analysis method that would give an unbiased quantification of the micro-vessel density.…”

Section: Introductionmentioning

confidence: 99%

Automated Angiogenesis Quantification through advanced Image Processing Techniques

Doukas

Chatziioannou

Papapetropoulos

2006

2006 International Conference of the IEEE Engineering in Medicine and Biology Society

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Abstract-Angiogenesis, the formation of blood vessels in tumors, is an interactive process between tumor, endothelial and stromal cells in order to create a network for oxygen and nutrients supply, necessary for tumor growth. According to this, angiogenic activity is considered a suitable method for both tumor growth or inhibition detection. The angiogenic potential is usually estimated by counting the number of blood vessels in particular sections. One of the most popular assay tissues to study the angiogenesis phenomenon is the developing chick embryo and its chorioallantoic membrane (CAM), which is a highly vascular structure lining the inner surface of the egg shell. The aim of this study was to develop and validate an automated image analysis method that would give an unbiased quantification of the micro-vessel density and growth in angiogenic CAM images. The presented method has been validated by comparing automated results to manual counts over a series of digital chick embryo photos. The results indicate the high accuracy of the tool, which has been thus extensively used for tumor growth detection at different stages of embryonic development.

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The effect of early prenatal hypercapnia on the vascular network in the chorioallantoic membrane of the chicken embryo

Verhoelst

Ketelaere

Decuypere

et al. 2011

Biotechnology Progress

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Over the last decade, the poultry sector has sought to develop novel ways to monitor chicken embryonic growth, health, and quality to control and optimize egg incubation conditions, particularly the concentration of dissolved gases (O(2), CO(2)). One of the parameters, which may change under different gas concentrations, is the angiogenesis in the chorioallantoic membrane (CAM), the organ for gas exchange of the chicken embryo. In this study, a newly developed methodology was used to quantify the angiogenesis in the CAM under normal and early hypercapnic conditions (i.e., increased CO(2) concentrations). Two experiments were conducted in which the same CO(2) profile was applied. The development of the vascular system was monitored from embryonic day (ED) 10 until ED 14 in Experiment 1, and until ED 16 in Experiment 2. This development was characterized by two different parameters-the vascular fraction (VF) as a measure for the density of the vascular network and the fractal dimension (FD) as a measure for the degree of branching of the vascular network. Moreover, in Experiment 2, embryo weights were compared between both groups. The proposed methodology showed that differences in the development of the vascular system could be observed across groups but also as function of the ED. Both VF and FD and the embryo weights were shown to be higher in the hypercapnia group compared to the control group.

show abstract

Quantitative assessment of angiogenesis in the chick embryo and its chorioallantoic membrane by computerised analysis of angiographic images

Cited by 29 publications

References 26 publications

Digital subtraction angiography and computer assisted image analysis for the evaluation of the antiangiogenetic effect of ionizing radiation on tumor angiogenesis

Digital subtraction angiography and computer assisted image analysis for the evaluation of the antiangiogenetic effect of ionizing radiation on tumor angiogenesis

Automated Angiogenesis Quantification through advanced Image Processing Techniques

The effect of early prenatal hypercapnia on the vascular network in the chorioallantoic membrane of the chicken embryo

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