QUANTITATIVE ASSAY OF THE MELANOPHORE DISPERSING HORMONE DURING THE DEVELOPMENT OF THE CHICKEN PITUITARY<sup>1</sup>

Rahn, Hermann; Drager, Glenn A.

doi:10.1210/endo-29-5-725

Cited by 19 publications

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“…Carbon dioxide has long been recognized as an important compound in the nutrition of bacteria (17,18) and protozoa (19). A biochemical basis for the role of carbon dioxide in nutrition was established when Wood and Werkman (20) demonstrated that it is fixed by heterotrophic bacteria.…”

Section: Discussionmentioning

confidence: 99%

The Role of Carbon Dioxide as an Essential Nutrient for Six Permanent Strains of Fibroblasts

Swim

Parker

1958

The Journal of Cell Biology

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The results of these studies demonstrate that carbon dioxide is required for the growth and maintenance of strains of fibroblasts derived from human tissues, strains FS4-705 and U12-705, from mouse tissue, strain L-705, and from rabbit tissues, strains RM3-56, RS1-56, and RT-56 in a chemically defined medium containing phosphite buffer in place of bicarbonate and supplemented with dialyzed serum and dialyzed embryo extract. Under these conditions, the cells fail to proliferate at a significant rate and begin to degenerate within 5 to 10 days when the flasks are not stoppered. Suflficient carbon dioxide is produced by the ceils to promote growth as indicated by the fact that maximal proliferation is obtained in the same phosphite media when stoppered flasks are employed. With the exception of RS1-56, all the remaining strains tested can be propagated serially in open flasks containing phosphite medium prepared with whole serum and embryo extract. The rate of growth under these conditions, however, is only one-half to onethird that obtained in stoppered flasks containing phosphite medium or the conventional bicarbonate medium.An earlier report from this laboratory described the propagation of several strains of mammalian cells in media containing fixed buffers such as tris(hydroxymethyl)aminomethane and glycylglycine in place of the conventional carbon dioxidebicarbonate system (1). Studies on the practical application of fixed buffers in tissue culture media were subsequently extended to include other compounds which are compatible with calcium and other cations and which are effective buffers in the p H range 7.0-8.0. In the course of these investigations, it was observed that several strains of fibroblasts proliferate at a reduced rate in certain media containing sodium phosphite (2) in place of bicarbonate when the flasks were dosed only by means of loosely fitting metal caps instead of stoppers. These observations suggested that carbon dioxide might be an essential nutrient for fibroblasts propagated in vitro. Some support for this * Aided by a grant (No. E-1547) from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, United States Public Health Service. speculation was indicated by other investigations which demonstrated that carbon dioxide is beneficial for the outgrowth of cells from explanted rat kidney (3, 4) and essential for explants of chick embryo tissue (5, 6). The results to be presented demonstrate that carbon dioxide is essential for the proliferation of six permanent strains of fibroblasts, derived from human, mouse, and rabbit tissues, when propagated in media containing dialyzed chick embryo extract and dialyzed horse serum. Materials and MethodsThe following strains of cells were propagated serially as described previously (7-9) : human fibroblasts, strains FS4-705 and U12-705 (8), rabbit fibroblasts, strains RM3-56, RS1-56, and RT6-56 (9) and mouse fibroblasts, strain L-705 (10). Medium 705 is composed of solution 703 (11) supplemented with 5 pe...

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Section: Discussionmentioning

confidence: 99%

The Role of Carbon Dioxide as an Essential Nutrient for Six Permanent Strains of Fibroblasts

Swim

Parker

1958

The Journal of Cell Biology

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“…Thyroidectomy affects not only the coloration but also the structure of regenerating feathers (170). The functional activ ity of the melanophore-dispersing pituitary hormone was followed through embryonic and posthatching stages of the chick (171).…”

Section: Hormones In Vertebratesmentioning

confidence: 99%

Developmental Physiology

Hamburger¹

1944

Annu. Rev. Physiol.

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The embryological and cytological basis of regional patterns in the definitive epithelial hypophysis of the chick,

Wilson¹

1952

Am. J. Anat.

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QUANTITATIVE ASSAY OF THE MELANOPHORE DISPERSING HORMONE DURING THE DEVELOPMENT OF THE CHICKEN PITUITARY¹

Cited by 19 publications

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The Role of Carbon Dioxide as an Essential Nutrient for Six Permanent Strains of Fibroblasts

The Role of Carbon Dioxide as an Essential Nutrient for Six Permanent Strains of Fibroblasts

Developmental Physiology

The embryological and cytological basis of regional patterns in the definitive epithelial hypophysis of the chick,

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QUANTITATIVE ASSAY OF THE MELANOPHORE DISPERSING HORMONE DURING THE DEVELOPMENT OF THE CHICKEN PITUITARY1

Cited by 19 publications

References 0 publications

The Role of Carbon Dioxide as an Essential Nutrient for Six Permanent Strains of Fibroblasts

The Role of Carbon Dioxide as an Essential Nutrient for Six Permanent Strains of Fibroblasts

Developmental Physiology

The embryological and cytological basis of regional patterns in the definitive epithelial hypophysis of the chick,

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QUANTITATIVE ASSAY OF THE MELANOPHORE DISPERSING HORMONE DURING THE DEVELOPMENT OF THE CHICKEN PITUITARY¹