Quantitative Application for SDS–PAGE in a Biochemistry Lab

Carter, John; Petersen, Brandon P.; Printz, Sarah A.; Sorey, Timothy; Kroll, Todd T.

doi:10.1021/ed300390j

Cited by 21 publications

(19 citation statements)

References 6 publications

(10 reference statements)

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“…SDS, as an anionic surfactant, is widely used in laundry, shampoos and pharmaceuticals 28 . SDS is also used for polyacrylamide gel electrophoresis to determine the molecular weight of proteins in biochemical experiments 29 . The concentrations of SDS were 1.14-3.92 μg/mL in river water samples collected from 12 stations of river in Turkey, 4-24 μg/mL in raw sewage of UK 30 .…”

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confidence: 99%

Exploring the Effects of Different Types of Surfactants on Zebrafish Embryos and Larvae

Wang

Zhang

et al. 2015

Sci Rep

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Currently, surfactants are widely distributed in the environment. As organic pollutants, their toxicities have drawn extensive attention. In this study, the effects of anionic [sodium dodecyl sulphate (SDS) ], cationic [dodecyl dimethyl benzyl ammonium chloride (1227)] and non-ionic [fatty alcohol polyoxyethylene ether (AEO) ] surfactants on zebrafish larval behaviour were evaluated. Five behavioural parameters were recorded using a larval rest/wake assay, including rest total, number of rest bouts, rest bouts length, total activity and waking activity. The results revealed that 1227 and AEO at 1 μg/mL were toxic to larval locomotor activity and that SDS had no significant effects. Moreover, we tested the toxicities of the three surfactants in developing zebrafish embryos. AEO exposure resulted in smaller head size, smaller eye size and shorter body length relative to SDS and 1227. All three surfactants incurred concentration-dependent responses. Furthermore, in situ hybridisation indicated that smaller head size may be associated with a decreased expression of krox20. The altered expression of ntl demonstrated that the developmental retardation stemmed from inhibited cell migration and growth. These findings provide references for ecotoxicological assessments of different types of surfactants, and play a warning role in the application of surfactants.

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confidence: 99%

Exploring the Effects of Different Types of Surfactants on Zebrafish Embryos and Larvae

Wang

Zhang

et al. 2015

Sci Rep

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“…SDS-PAGE has frequently been used for determination of a given protein band and the molecular mass of proteins. [ 28 ] In the present study, 26 natural CS samples were analyzed by the 12% SDS-PAGE [ Figure 1 ]. As shown in Figure 1 , the molecule weights of major proteins in CS samples were from 6.5 to 97.2 kDa.…”

Section: Resultsmentioning

confidence: 99%

Analysis of soluble proteins in natural Cordyceps sinensis from different producing areas by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and two-dimensional electrophoresis

Zuo

Zhang

et al. 2017

Phcog Res

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Background:As one of the bioactive components in Cordyceps sinensis (CS), proteins were rarely used as index components to study the correlation between the protein components and producing areas of natural CS.Objective:Protein components of 26 natural CS samples produced in Qinghai, Tibet, and Sichuan provinces were analyzed and compared to investigate the relationship among 26 different producing areas.Materials and Methods:Proteins from 26 different producing areas were extracted by Tris-HCl buffer with Triton X-100, and separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis (2-DE).Results:The SDS-PAGE results indicated that the number of protein bands and optical density curves of proteins in 26 CS samples was a bit different. However, the 2-DE results showed that the numbers and abundance of protein spots in protein profiles of 26 samples were obviously different and showed certain association with producing areas.Conclusions:Based on the expression values of matched protein spots, 26 batches of CS samples can be divided into two main categories (Tibet and Qinghai) by hierarchical cluster analysis.SUMMARY The number of protein bands and optical density curves of proteins in 26 Cordyceps sinensis samples were a bit different on the sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein profilesNumbers and abundance of protein spots in protein profiles of 26 samples were obvious different on two-dimensional electrophoresis mapsTwenty-six different producing areas of natural Cordyceps sinensis samples were divided into two main categories (Tibet and Qinghai) by Hierarchical cluster analysis based on the values of matched protein spots. Abbreviations Used: SDS-PAGE: Sodium dodecyl sulfate polyacrylamide gel electrophoresis, 2-DE: Two-dimensional electrophoresis, Cordyceps sinensis: CS, TCMs: Traditional Chinese medicines

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“…Check the protein expression and protein purity with 10 µL of sample by running an SDS-PAGE. Determine the protein concentration by generating a standard curve of bovine serum albumin (BSA) [ 16 ]. Prepare serial dilution, ranging from 0.31 to 1 mg/mL, from a stock solution of 10 mg/mL BSA.…”

Section: Methodsmentioning

confidence: 99%

“…Determine the protein concentration by generating a standard curve of bovine serum albumin (BSA) [16]. Prepare serial dilution, ranging from 0.31 to 1 mg/mL, from a stock solution of 10 mg/mL BSA.…”

Section: Critical Stepmentioning

confidence: 99%

Synthesis of Quenchbodies for One-Pot Detection of Stimulant Drug Methamphetamine

Jeong

Dong

Yeom

et al. 2020

MPs

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The problem of illicit drug use and addiction is an escalating issue worldwide. As such, fast and precise detection methods are needed to help combat the problem. Herein, the synthesis method for an anti-methamphetamine Quenchbody (Q-body), a promising sensor for use in simple and convenient assays, has been described. The fluorescence intensity of the Q-body generated by two-site labeling of Escherichia coli produced anti-methamphetamine antigen-binding fragment (Fab) with TAMRA-C2-maleimide dyes increased 5.1-fold over background in the presence of a hydroxyl methamphetamine derivative, 3-[(2S)-2-(methylamino)propyl]phenol. This derivative has the closest structure to methamphetamine of the chemicals available for use in a laboratory. Our results indicate the potential use of this Q-body as a novel sensor for the on-site detection of methamphetamine, in such occasions as drug screening at workplace, suspicious substance identification, and monitoring patients during drug rehabilitation.

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Quantitative Application for SDS–PAGE in a Biochemistry Lab

Cited by 21 publications

References 6 publications

Exploring the Effects of Different Types of Surfactants on Zebrafish Embryos and Larvae

Exploring the Effects of Different Types of Surfactants on Zebrafish Embryos and Larvae

Analysis of soluble proteins in natural Cordyceps sinensis from different producing areas by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and two-dimensional electrophoresis

Synthesis of Quenchbodies for One-Pot Detection of Stimulant Drug Methamphetamine

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