Quantitative and sensitive detection of SARS coronavirus nucleocapsid protein using quantum dots‐conjugated RNA aptamer on chip

Roh, Changhyun; Jo, Sung-Kee

doi:10.1002/jctb.2721

Cited by 118 publications

(88 citation statements)

References 31 publications

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“…This miniaturization device based on optical QDs-RNA aptamer chip can detect SARS-CoV N protein at the concentrations of as low as 0.1 pg mL −1 . The suggested graphical SARS-CoV N protein method enjoys high sensitivity, accuracy, simplicity, and easy operation [ 170 ]. Table 2 presents a comparison between different methods for the detection of coronaviruses.…”

Section: Detection Methodsmentioning

confidence: 99%

An Overview on SARS-CoV-2 (COVID-19) and Other Human Coronaviruses and Their Detection Capability via Amplification Assay, Chemical Sensing, Biosensing, Immunosensing, and Clinical Assays

et al. 2020

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A novel coronavirus of zoonotic origin (SARS-CoV-2) has recently been recognized in patients with acute respiratory disease. COVID-19 causative agent is structurally and genetically similar to SARS and bat SARS-like coronaviruses. The drastic increase in the number of coronavirus and its genome sequence have given us an unprecedented opportunity to perform bioinformatics and genomics analysis on this class of viruses. Clinical tests like PCR and ELISA for rapid detection of this virus are urgently needed for early identification of infected patients. However, these techniques are expensive and not readily available for point-of-care (POC) applications. Currently, lack of any rapid, available, and reliable POC detection method gives rise to the progression of COVID-19 as a horrible global problem. To solve the negative features of clinical investigation, we provide a brief introduction of the general features of coronaviruses and describe various amplification assays, sensing, biosensing, immunosensing, and aptasensing for the determination of various groups of coronaviruses applied as a template for the detection of SARS-CoV-2. All sensing and biosensing techniques developed for the determination of various classes of coronaviruses are useful to recognize the newly immerged coronavirus, i.e., SARS-CoV-2. Also, the introduction of sensing and biosensing methods sheds light on the way of designing a proper screening system to detect the virus at the early stage of infection to tranquilize the speed and vastity of spreading. Among other approaches investigated among molecular approaches and PCR or recognition of viral diseases, LAMP-based methods and LFAs are of great importance for their numerous benefits, which can be helpful to design a universal platform for detection of future emerging pathogenic viruses.

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Section: Detection Methodsmentioning

confidence: 99%

An Overview on SARS-CoV-2 (COVID-19) and Other Human Coronaviruses and Their Detection Capability via Amplification Assay, Chemical Sensing, Biosensing, Immunosensing, and Clinical Assays

et al. 2020

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“…In another study, Roh and Jo designed a chip that detected SARS-CoV N proteins in only an hour with a detection limit of 0.1 pg/ml. The target proteins were immobilized on the chip via ProLinker™ capture probes, then quantum dots-labeled specific aptamers bound to the targets, and fluorescence intensity was evaluated using confocal microscopy [139]. In a recent study, Diao and colleagues proposed a fluorescent immunochromatographic strip assay that detected the N proteins in nasopharyngeal aspirate and urine samples of COVID-19 patients that took only 10 min with a sensitivity of 68-98%.…”

Section: Optical Biosensorsmentioning

confidence: 99%

Laboratory detection methods for the human coronaviruses

Shabani

Dowlatshahi

Abdekhodaie

2020

Eur J Clin Microbiol Infect Dis

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Coronaviruses are a group of envelop viruses which lead to diseases in birds and mammals as well as human. Seven coronaviruses have been discovered in humans that can cause mild to lethal respiratory tract infections. HCoV-229E, HCoV-OC43, HCoV-NL63, and HCoV-HKU1 are the low-risk members of this family and the reason for some common colds. Besides, SARS-CoV, MERS-CoV, and newly identified SARS-CoV-2, which is also known as 2019-nCoV, are the more dangerous viruses. Due to the rapid spread of this novel coronavirus and its related disease, COVID-19, a reliable, simple, fast, and low-cost detection method is necessary for patient diagnosis and tracking worldwide. Human coronaviruses detection methods were classified and presented in this article. The laboratory detection techniques include RT-PCR, RT-LAMP, electrochemical and optical biosensors for RNA detection, and whole virus or viral proteins detection assays.

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“…This strategy offers many advantages; the DL-DNA can be used as both a structural scaffold and a probe while its detection mechanism relies on fluorescent color ratios instead of single color and can be easily adapted to a fluorescence detection system. Moreover, Roh et al reported a testing protocol using the system on a chip (SoC) technology whose detection mechanism is based on QDs-conjugated RNA aptamer [ 240 ]. The amine group of the RNA aptamer was covalently attached to the commercially available, fluorescent carboxyl terminated QDs605, usually made of a nanometer-scale CdSe core and ZnS shell to better control and modulate their optical response.…”

Section: Diagnostics and Biosensors For Covid-19mentioning

confidence: 99%

Biomedical Science to Tackle the COVID-19 Pandemic: Current Status and Future Perspectives

et al. 2020

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The coronavirus infectious disease (COVID-19) pandemic emerged at the end of 2019, and was caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), which has resulted in an unprecedented health and economic crisis worldwide. One key aspect, compared to other recent pandemics, is the level of urgency, which has started a race for finding adequate answers. Solutions for efficient prevention approaches, rapid, reliable, and high throughput diagnostics, monitoring, and safe therapies are needed. Research across the world has been directed to fight against COVID-19. Biomedical science has been presented as a possible area for combating the SARS-CoV-2 virus due to the unique challenges raised by the pandemic, as reported by epidemiologists, immunologists, and medical doctors, including COVID-19’s survival, symptoms, protein surface composition, and infection mechanisms. While the current knowledge about the SARS-CoV-2 virus is still limited, various (old and new) biomedical approaches have been developed and tested. Here, we review the current status and future perspectives of biomedical science in the context of COVID-19, including nanotechnology, prevention through vaccine engineering, diagnostic, monitoring, and therapy. This review is aimed at discussing the current impact of biomedical science in healthcare for the management of COVID-19, as well as some challenges to be addressed.

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Quantitative and sensitive detection of SARS coronavirus nucleocapsid protein using quantum dots‐conjugated RNA aptamer on chip

Abstract: Here, a new quantum dots (QDs)-conjugated RNA aptamer with high sensitivity and rapidity is proposed for the detection of SARS-CoV N protein using an on chip system.

Cited by 118 publications

References 31 publications

An Overview on SARS-CoV-2 (COVID-19) and Other Human Coronaviruses and Their Detection Capability via Amplification Assay, Chemical Sensing, Biosensing, Immunosensing, and Clinical Assays

An Overview on SARS-CoV-2 (COVID-19) and Other Human Coronaviruses and Their Detection Capability via Amplification Assay, Chemical Sensing, Biosensing, Immunosensing, and Clinical Assays

Laboratory detection methods for the human coronaviruses

Biomedical Science to Tackle the COVID-19 Pandemic: Current Status and Future Perspectives

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