Quantitative and scanning electron microscope observations on the attachment of Pseudomonas tolaasii and other bacteria to the surface of Agaricus bisporus

Preece, T.F.; Wong, Wendy

doi:10.1016/0048-4059(82)90043-1

Cited by 44 publications

(28 citation statements)

References 9 publications

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“…Once present, this bacterium is able to attach the mycelial surface of developing A. bisporus (23,25). Under UV light, colonies of the isolated pseudomonas species produced yellow to yellowish-green fluorescent color.…”

Section: Discussionmentioning

confidence: 99%

Pathogenic variation in isolates of Pseudomonas causing the brown blotch of cultivated mushroom, Agaricus bisporus

Abou-Zeid¹

2012

Braz. J. Microbiol.

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Twenty seven bacterial isolates were isolated from superficial brown discolorations on the caps of cultivated Agaricus bisporus. After White Line Assay (WLA) and the assist of Biolog computer-identification system, isolates were divided into groups: (I) comprised ninteen bacterial isolates that positively responded to a Pseudomonas "reactans" reference strain (NCPPB1311) in WLA and were identified as Pseudomonas tolaasii, (II) comprised two isolates which were WLA+ towards the reference strain (JCM21583) of P.tolaasii and were proposed to be P. "reactans". The third group comprised six isolates, two of which weakly responded to the strain of P. tolaasii and were identified as P. gingeri whereas the other four were WLAand identified as P. fluorescens (three isolates) and P. marginalis (one isolate). Isolates of P. tolaasii showed high aggressiveness compared with those of P. "reactans" in pathogenicity tests. Cubes of 1 cm 3 of A. bisporus turned brown and decreased in size when were inoculated with 10 µl of P. tolaasii suspension containing 10 8 CFU ml, whereas a similar concentration of P. "reactans" caused only light browning.Fifty µl of the same concentration of P. tolaasii isolates gave typical brown blotch symptoms on fresh mushroom sporophores whereas the two P. "reactans" isolates caused superficial light discoloration only after inoculation with 100 µl of the same concentration. Mixture from both bacterial suspensions increased the brown areas formed on the pileus. This is the first pathogenicity report of P. tolasii and P. "reactans" isolated from cultivated A. bisporus in Egypt.

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Section: Discussionmentioning

confidence: 99%

Pathogenic variation in isolates of Pseudomonas causing the brown blotch of cultivated mushroom, Agaricus bisporus

Abou-Zeid¹

2012

Braz. J. Microbiol.

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“…The fact that tolaasin-detoxifying bacteria were released into water from fungal fruit bodies without disrupting the fungal cells indicates that these bacteria are attached to the surface of fungal mycelia like P. tolaasii rather than residing in the fungal protoplasm. 33) Burkholderia, an rRNA group II pseudomonad, was recently recognized inside of spores of arbascular-mycorrhizal fungi, the biological contribution of which to host fungi has been yet unknown.…”

Section: Discussionmentioning

confidence: 99%

Identification of Non-Pseudomonad Bacteria from Fruit Bodies of Wild Agaricales Fungi That Detoxify Tolaasin Produced byPseudomonas tolaasii

Tsukamoto

Murata

Shirata

2002

Bioscience, Biotechnology, and Biochemistry

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“…Tolaasin causes disruption of cell membranes from a range of cell types and has both ion channel-forming and biosurfactant properties (6,26,38). The ability of P. tolaasii to show a positive chemotactic response to A. bisporus exudates (20) and its ability to attach to mycelial surfaces (9,35,37) may also be important as steps in the early stages of development of brown blotch disease.…”

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confidence: 99%

Identification and characterization of a locus which regulates multiple functions in Pseudomonas tolaasii, the cause of brown blotch disease of Agaricus bisporus

1995

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Pseudomonas tolaasii, the causal agent of brown blotch disease of Agaricus bisporus, spontaneously gives rise to morphologically distinct stable sectors, referred to as the phenotypic variant form, at the margins of the wild-type colonies. The phenotypic variant form is nonpathogenic and differs from the wild type in a range of biochemical and physiological characteristics. A genomic cosmid clone (pSISG29) from a wild-type P. tolaasii library was shown to be capable of restoring a range of characteristics of the phenotypic variant to those of the wild-type form, when present in trans. Subcloning and saturation mutagenesis analysis with Tn5lacZ localized a 3.0-kb region from pSISG29, designated the pheN locus, required for complementation of the phenotypic variant to the wild-type form. Pseudomonas tolaasii causes the economically important brown blotch disease of the mushroom Agaricus bisporus (Lange) Imbach (53). Synthesis of the low-molecular-weight (M r , 1,985) lipodepsipeptide extracellular toxin tolaasin by P. tolaasii is primarily responsible for eliciting disease symptoms (6,38,39). Tolaasin causes disruption of cell membranes from a range of cell types and has both ion channel-forming and biosurfactant properties (6,26,38). The ability of P. tolaasii to show a positive chemotactic response to A. bisporus exudates (20) and its ability to attach to mycelial surfaces (9, 35, 37) may also be important as steps in the early stages of development of brown blotch disease.P. tolaasii undergoes phenotypic variation, a strategy frequently used by bacteria to survive disparate environmental conditions. Old colonies of P. tolaasii frequently produce sectors which, when subcultured onto fresh medium, show different phenotypically distinct colonial forms (10, 29, 36). The wild-type strain is opaque, mucoid, pathogenic, and nonfluorescent, whereas the sectors are translucent, nonmucoid, nonpathogenic, and fluorescent (10, 57). In addition to alteration in virulence, qualitative analysis has revealed that a number of biochemical properties are also changed in the transition from the wild type to a phenotypic variant. These include the loss of the ability to produce tolaasin, hydrolyze casein, and grow normally on medium containing cetrimide (10). Finally, the variant form swims faster and shows a more rapid chemotactic response than the wild type (20). Phase change from the pathogenic to the nonpathogenic form in P. tolaasii has been referred to as a smooth-to-rough transition (10). This description generally designates a difference in the lipopolysaccharide (LPS) content of the two forms (16,29). Recent studies by Hutchison (25) have demonstrated that there is no difference in the LPS profiles of the smooth and rough forms of P. tolaasii. Therefore, the terms ''wild type'' and ''phenotypic variant'' are used here instead of ''smooth'' and ''rough,'' respectively.The aim of this study was to identify the molecular switch controlling the transition from the wild type to the phenotypic variant in P. tolaasii. First, we ...

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Quantitative and scanning electron microscope observations on the attachment of Pseudomonas tolaasii and other bacteria to the surface of Agaricus bisporus

Cited by 44 publications

References 9 publications

Pathogenic variation in isolates of Pseudomonas causing the brown blotch of cultivated mushroom, Agaricus bisporus

Pathogenic variation in isolates of Pseudomonas causing the brown blotch of cultivated mushroom, Agaricus bisporus

Identification of Non-Pseudomonad Bacteria from Fruit Bodies of Wild Agaricales Fungi That Detoxify Tolaasin Produced byPseudomonas tolaasii

Identification and characterization of a locus which regulates multiple functions in Pseudomonas tolaasii, the cause of brown blotch disease of Agaricus bisporus

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