Quantitative and qualitative determination of six xanthones in Garcinia mangostana L. by LC–PDA and LC–ESI-MS

Ji, Xiuhong; Avula, Bharathi; Khan, Ikhlas A.

doi:10.1016/j.jpba.2006.10.018

Cited by 105 publications

(46 citation statements)

References 26 publications

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“…The results are shown in Figure 2 and Table1. Through an analysis of standard xanthones samples and a literature survey [30,34], we confirmed that the main peaks appearing after extraction were 3-isomangostin, mangostanol, 8-desoxygartanin, gartanin, α-mangostin, garcinone E, 9-hydroxycalabaxanthone and β-mangostin, respectively. To investigate the composition dependency on the parts of the mangosteen pericarp, we cut the pericarps into exocarp and mesocarp+endocarp.…”

Section: Resultsmentioning

confidence: 53%

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Extraction of Xanthones from the Pericarps of <i>Garcinia mangostana</i> Linn. with Supercritical Carbon Dioxide and Ethanol

Mishima

Kawakami

Yokota

et al. 2013

SERDJ

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The extraction of xanthones, such as α-mangostin from the pericarp of mangosteen (Garcinia mangostana Linn.) by supercritical fluid extraction (SFE), for which the solvent was carbon dioxide (CO 2 ), was carried out at 35, 40 and 50 ºC. The extraction pressure was from 10 to 20 MPa. In order to enhance the yield of the extraction, ethanol was added as an entrainer. The yields of xanthones, such as α-mangostin in the extraction were significantly improved, whereas a change in the selectivity of the extract was not observed. We also conducted qualitative and quantitative analyses for xanthones in the extract by HPLC, and analyzed the extraction behavior. The effect of three operating parameters, such as temperature, pressure and the mole fraction of ethanol in a supercritical solution of CO 2 on the extraction yield was investigated using the single-factor method.

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Section: Resultsmentioning

confidence: 53%

“…Previously many researchers proposed a variety of HPLC profiles to separate the components in the extraction composite more accurately [12,18,19,33,34]. We applied the modified method that Zarena et al originally developed [30].…”

Section: Resultsmentioning

confidence: 99%

Extraction of Xanthones from the Pericarps of <i>Garcinia mangostana</i> Linn. with Supercritical Carbon Dioxide and Ethanol

Mishima

Kawakami

Yokota

et al. 2013

SERDJ

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“…7.12 min as compared to 16.23 min which attributed to the length and the size of silica beads. On the other hand, Ji X et al [30] and Yodhnu et al [31] had also validated an analytical HPLC method for determination the concentration of alpha-mangostin in the fruit rind of GM using isocratic mobile phase composed of 0.2% formic acid and acetonitrile. Although their chromatographic separation was performed on a slightly longer column (125 mm) as compared to ours (100 mm), their retention time was slightly faster (6.2 min) than ours.…”

Section: Validation Proceduresmentioning

confidence: 99%

Development and Validation of Analytical Method by RP-HPLC for Quantification of Alpha-Mangostin Encapsulated in PLGA Microspheres

Ali¹

2012

J Anal Bioanal Techniques

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A simple, rapid, precise and highly accurate RP-HPLC method was developed and validated for determination of alpha-mangostin content extracted from PLGA-microspheres. Method was developed using a silica-based deactivated C-18 column (4.6×100 mm, 3 μm) with a mobile phase of 70-80 % v/v acetonitrile (A) and 0.1% v/v orthophosphoric acid (B), with the following pre-determined timed-gradient program: 70% (A) isocratic for 6 min, 70-75% (A) in 1.2 min, 75-80% (A) in 0.4 min, 80% (A) isocratic for 2.4 min, 80-70% (A) in 0.4 min, finally 70% (A) isocratic for 5 min, with a flow rate of 1 mL/min, detected at 320 nm by a UV detector. Linearity was obtained over the range of 1-200 μg/mL with r 2 =0.9995. The precision was achieved based on repeatability and intermediate precision with RSD of 0.13-0.6% and 0.57-1.2%, respectively. Percent recovery of 100.55% to 103.82% with RSD 0.086 -0.15 implied high accuracy of the method. Limit of detection and limit of quantitation were 0.038 and 0.121 μg/ml, respectively suggesting good sensitivity of the method. The method is envisaged to be effectively used for routine quality control assay for encapsulated alpha-mangostin in PLGA microspheres.

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“…The tree is cultivated for centuries in the tropical rainforests of some Asian countries. The tree can be found now in Northern Australia, Brazil, Central America, Hawaii, Southern India, Indonesia, Malaysia, Thailand and other tropical countries (Ji et al, 2007). The fruit is dark purple or reddish with soft, white and juicy edible pulp.…”

Section: Introductionmentioning

confidence: 99%

“…The pericarps of the fruit have been used in folk medicine for the treatment of several human diseases including skin and wound infections, hemorrhoids, arthritis, tuberculosis, inflammation, mycosis, ulcers, genitourinary tract infections, fever, amoebic dysentery and abdominal pain (Baxter et al, 1999;Caius, 2003;Moongkarndi et al, 2004;Salguero, 2003;Suksamrarn et al, 2006). Mangosteen is also used worldwide as an ingredient of several commercial products including nutritional supplements, herbal cosmetics and pharmaceutical products (Ji et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Antitumorigenicity of xanthones-rich extract from Garcinia mangostana fruit rinds on HCT 116 human colorectal carcinoma cells

Aisha¹,

Abu‐Salah²,

Nassar³

et al. 2011

Rev. bras. farmacogn.

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This study aimed to investigate the antitumorigenicity of xanthones-rich extract from Garcinia mangostana L., Clusiaceae, fruit rinds which was obtained by a simple recrystallization of 75% ethanolic extract. α-Mangostin content of the extract was determined qualitatively by TLC and quantitatively by HPLC, and total xanthones content was quantified by UV spectrophotometry. The extract was evaluated for cytotoxicity, apoptosis and antitumorigenicity on HCT 116 human colorectal carcinoma cells. α-Mangostin was found to be the main constituent of the extract which was 71.2±0.1%, and the total xanthones content was 95±4.8% (wt/ wt). The extract showed potent dose dependent cytotoxicity with IC50 value 9.2 µg/ mL. Apoptosis studies revealed activation of caspases 3 and 7, DNA fragmentation, chromatin condensation and loss of mitochondrial membrane potential. Studies on cell migration and colony formation indicate reduced cell migration ability and clonogenicity of treated HCT 116 cells at sub-inhibitory concentrations. Taken together, the cytotoxic effect of the xanthones extract is mediated through the mitochondrial pathway of apoptosis. The reduced cell migration and clonogenicity of HCT 116 cells might prevent both primary and metastatic tumor growth in vivo which will be the topic of our future work using the metastatic orthotopic colon cancer model.

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Quantitative and qualitative determination of six xanthones in Garcinia mangostana L. by LC–PDA and LC–ESI-MS

Cited by 105 publications

References 26 publications

Extraction of Xanthones from the Pericarps of <i>Garcinia mangostana</i> Linn. with Supercritical Carbon Dioxide and Ethanol

Extraction of Xanthones from the Pericarps of <i>Garcinia mangostana</i> Linn. with Supercritical Carbon Dioxide and Ethanol

Development and Validation of Analytical Method by RP-HPLC for Quantification of Alpha-Mangostin Encapsulated in PLGA Microspheres

Antitumorigenicity of xanthones-rich extract from Garcinia mangostana fruit rinds on HCT 116 human colorectal carcinoma cells

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