Quantitative and qualitative analysis of microorganisms in an assisted reproductive technology facility

Herlong, J.L.; Reubish, Ken; Higdon, H. Lee; Boone, William R.

doi:10.1016/j.fertnstert.2007.04.019

Cited by 14 publications

(5 citation statements)

References 27 publications

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“…A more recent study shows that the contamination rate of syringes is lower when they are prepared in a controlled environment as compared to a non-controlled environment (Stucki et al 2009). Similar results were found when monitoring an assisted reproductive technology facility (Herlong et al 2008) and a peripheral blood progenitor cell (PBPC) products facility (Ritter et al 2003).…”

Section: Discussionsupporting

confidence: 62%

Cleanrooms and tissue banking how happy I could be with either GMP or GTP?

et al. 2013

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The regulatory framework of tissue banking introduces a number of requirements for monitoring cleanrooms for processing tissue or cell grafts. Although a number of requirements were clearly defined, some requirements are open for interpretation. This study aims to contribute to the interpretation of GMP or GTP guidelines for tissue banking. Based on the experience of the participating centers, the results of the monitoring program were evaluated to determine the feasibility of a cleanroom in tissue banking and the monitoring program. Also the microbial efficacy of a laminar airflow cabinet and an incubator in a cleanroom environment was evaluated. This study indicated that a monitoring program of a cleanroom at rest in combination with (final) product testing is a feasible approach. Although no statistical significance (0.90 \ p \ 0.95) was found there is a strong indication that a Grade D environment is not the ideal background environment for a Grade A obtained through a laminar airflow cabinet. The microbial contamination of an incubator in a cleanroom is limited but requires closed containers for tissue and cell products.

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Section: Discussionsupporting

confidence: 62%

Cleanrooms and tissue banking how happy I could be with either GMP or GTP?

et al. 2013

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“…With the reduction in microorganism density achieved through the construction of a clean room, further preventive measures are improved, such as low antibiotic levels needed within the culture medium. 43 …”

Section: Resultsmentioning

confidence: 99%

Effect of the Air Filtration System Replacement on Embryo Quality in the Assisted Reproduction Laboratory

Poletto

Lima

Approbato

2018

Rev Bras Ginecol Obstet

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Improving infrastructural conditions of the in vitro fertilization laboratory, such as the air quality, has profound positive effects on embryo culture. Poor environmental conditions reduce the rate of embryo formation and, therefore, of pregnancy. This review article presents important publications regarding the impact of air quality in human reproduction laboratories on embryo quality, pregnancy success, and live births. The studies demonstrate that the replacing the air filtration system improves significantly the environmental air quality, and, consequently, improves laboratory parameters, such as the fertilization rate, the number of blastocysts, the embryo implantation rate, and the number of live births. On the other hand, improving air quality decreases the number of abortions. Therefore, environmental parameters that improve embryo quality and increase healthy child birth rates must be the main targets for the assisted reproduction laboratory quality control.

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“…Current methods used to assess bio-aerosols in indoor environments include sampling onto filters from which microorganisms are mechanically removed into solution and cultured (Eduard and Heederik 1998), impaction onto solid agar or liquid impingement followed by culture (Juozaitis et al 1994).The use of settle plates for environmental sampling appears to be the most common technique identified in this review. In the identified studies, settle plates were left in locations, such as on the floor or on shelves for time periods ranging from 10 min in a study by Sarica et al (2002) to 24 h in a study by Herlong et al (2008) in an assisted reproduction facility. However, the majority of settle plates were left for between 31 min and 1 h.…”

Section: Search Resultsmentioning

confidence: 99%

Healthy schools: standardisation of culturing methods for seeking airborne pathogens in bioaerosols emitted from human sources

et al. 2012

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Schools are one of the critical social infrastructures in a society, and children are particularly at risk of lung damage and infection caused by poor air quality. The issue of healthy schools is a global concern. In this paper, we highlight the importance of airborne transmission of microorganisms in schools and the impact on children's health and wider economic and social implications. We propose the concept of air hygiene; measuring and assessing the level of bioaerosols emitted from human sources (primarily respiratory) can serve as a guide to indicate overall air quality, associated with airborne infections. Current bioaerosol sampling in schools (if carried out) is based on the use of Trypticase Soy Agar that can indicate environmental sources of bioaerosols. We suggest that a more appropriate agar could be used for bioaerosols of human origin. It is critical to develop and standardise a simple and economic method that can be used in a range of schools and environments even if resources are limited to monitor the air hygiene. The use of blood agar and different samplings methods are reviewed, and the most appropriate method is proposed for further testing and validation in order to develop a global standard method.

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Quantitative and qualitative analysis of microorganisms in an assisted reproductive technology facility

Cited by 14 publications

References 27 publications

Cleanrooms and tissue banking how happy I could be with either GMP or GTP?

Cleanrooms and tissue banking how happy I could be with either GMP or GTP?

Effect of the Air Filtration System Replacement on Embryo Quality in the Assisted Reproduction Laboratory

Healthy schools: standardisation of culturing methods for seeking airborne pathogens in bioaerosols emitted from human sources

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