Quantitative and qualitative analysis of proteins in fresh frozen plasma obtained from whole blood donations and prepared with two photochemical treatments
“…We were able to confirm that the activities of most plasma coagulation factors and antithrombotic proteins were affected, but only moderately, by the IBS procedure. Only FVII and FVIII displayed a somewhat lower degree of retention of their initial pre‐IBS activity (67%), in accordance with previous studies . For all factors, the values were well within the reference ranges and met the requirements of the Guide to the Preparation, Use and Quality Assurance of Blood Components published by the Directorate for the Quality of Medicine and Health Care of the Council of Europe (EDQM) .…”
The IBS technique for plasma moderately decreases the activities of plasma coagulation factors and antithrombotic proteins, with no impact on the thrombin generation potential of plasma and very limited modifications of the proteomic profile.
“…We were able to confirm that the activities of most plasma coagulation factors and antithrombotic proteins were affected, but only moderately, by the IBS procedure. Only FVII and FVIII displayed a somewhat lower degree of retention of their initial pre‐IBS activity (67%), in accordance with previous studies . For all factors, the values were well within the reference ranges and met the requirements of the Guide to the Preparation, Use and Quality Assurance of Blood Components published by the Directorate for the Quality of Medicine and Health Care of the Council of Europe (EDQM) .…”
The IBS technique for plasma moderately decreases the activities of plasma coagulation factors and antithrombotic proteins, with no impact on the thrombin generation potential of plasma and very limited modifications of the proteomic profile.
“…A direct comparison of plasma quality after MB and AS treatment was done in two previous studies, both using a pool‐and‐split design with 12 replicate tests . Whereas Osselaer and coworkers report a generally stronger degrading effect of MB than AS, this was not the case in our study.…”
Section: Discussionmentioning
confidence: 50%
“…Only the FV and FXI results were uniformly more favorable for AS treatment. Cid and colleagues found no significant differences in their smaller set of comparisons.…”
PR treatment of plasma affects coagulation proteins and coagulant capacity. For the RF method this effect is most pronounced, although to some extent compensated by a smaller volume loss.
“…The loss of coagulation factors seen in FFP following PI treatment is well documented by several authors . The design of our study allowed the direct comparison of two PI methods, as well as accounting for dilution factors and volume losses.…”
MB-cryo contained a significantly higher content of FVIII and lower content of FXIII when compared to AS-cryo, with no difference in fibrinogen activity.
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