Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana

Adachi, Sumiko; Nobusawa, Takashi; Umeda, Masaaki

doi:10.1016/j.ydbio.2009.03.002

Cited by 23 publications

(18 citation statements)

References 44 publications

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“…Using Arabidopsis thaliana tissues for studying the promoter activity of CDKA;1, Adachi et al (2009) revealed two types of transcriptional control in shoots: general quantitative regulation and cell type-specific regulation. In this study, Denca;CDK;1 transcripts are expressed across the five tissue samples by the order of PLBs>roots>seeds>stems>leaves (Fig.…”

Section: Discussionmentioning

confidence: 99%

Characterization of an A-type cyclin-dependent kinase gene from Dendrobium candidum

et al. 2012

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AbstractCyclin-dependent kinases (CDKs) play an essential role in cell cycle regulation during the embryonic and postembryonic development of organisms. To better understand the molecular mechanisms of CDKs involved in embryogenesis regulation in the endangered medicinal plant Dendrobium candidum Wall. ex Lindl., a 1229-bp full-length cDNA of an A-type CDK gene, Denca;CDKA;1, was identified using 3′ rapid amplification of cDNA end (RACE) PCR. Denca;CDKA;1 was predicted to encode a 294 amino acid residue-long protein of 33.76 kDa with an isoelectric point of 7.72. The deduced Denca;CDKA;1 protein contained a conserved serine/threonine-protein kinase domain (S-TKc) and a canonical cyclinbinding “PSTAIRE” motif. Multiple sequence alignment indicated that members of CDKA family from various plants exhibited a high degree of sequence identity ranging from 82% to 93%. A neighbor-joining phylogenetic tree showed that Denca;CDKA;1 was clustered into the plant group and was distant from the animal and fungal groups. The modeled three-dimensional structure of Denca;CDKA;1 exhibited the similar functional structure of a fold consisting of β-sheets and α-helices joined by discontinuous random coils forming two relatively independent lobes. Quantitative real-time PCR analysis revealed that Denca;CDKA;1 transcripts were the most abundant in protocorm-like bodies with 4.76 fold, followed by that in roots (4.19 fold), seeds (2.57 fold), and stems (1.57 fold). This study characterized the novel Denca;CDKA;1 gene from D. candidum for the first time and the results will be useful for further functional determination of the gene.

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Section: Discussionmentioning

confidence: 99%

Characterization of an A-type cyclin-dependent kinase gene from Dendrobium candidum

et al. 2012

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“…Fixation of the samples was conducted as described50 with minor changes. Samples were dehydrated with an ethanol series, and then ethanol was substituted with mixture containing Technovit 7100 solution (Heraeus Kulzer, Tokyo, Japan) and hardener I (Heraeus Kulzer, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

The jasmonate-responsive GTR1 transporter is required for gibberellin-mediated stamen development in Arabidopsis

et al. 2015

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Plant hormones are transported across cell membranes during various physiological events. Recent identification of abscisic acid and strigolactone transporters suggests that transport of various plant hormones across membranes does not occur by simple diffusion but requires transporter proteins that are strictly regulated during development. Here, we report that a major glucosinolate transporter, GTR1/NPF2.10, is multifunctional and may be involved in hormone transport in Arabidopsis thaliana . When heterologously expressed in oocytes, GTR1 transports jasmonoyl-isoleucine and gibberellin in addition to glucosinolates. gtr1 mutants are severely impaired in filament elongation and anther dehiscence resulting in reduced fertility, but these phenotypes can be rescued by gibberellin treatment. These results suggest that GTR1 may be a multifunctional transporter for the structurally distinct compounds glucosinolates, jasmonoyl-isoleucine and gibberellin, and may positively regulate stamen development by mediating gibberellin supply.

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“…In the wild type, precise modulation of KRPs expression might be critical for the development of flat leaves. Adachi et al (2009) reported that the CDKA;1 promoter region contains a regulatory region that controls abaxial side-biased expression. Our microarray analyses, however, showed that levels of CDKA;1 transcripts were not significantly increased in shoot apices of mutant plants forming abaxialized filamentous leaves (our unpublished data).…”

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confidence: 99%

ASYMMETRIC LEAVES2 and FASCIATA2 cooperatively regulate the formation of leaf adaxial-abaxial polarity in Arabidopsis thaliana

Ishibashi

Machida

2013

Plant Biotechnology

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The establishment of adaxial-abaxial polarity in the early stage of leaf development is important for the expansion of lamina. In Arabidopsis thaliana, asymmetric leaves2 (as2) and as1 mutations cause defects in the leaf adaxialabaxial polarity, which are exhibited as abaxialized filamentous leaves in the various modifier mutant backgrounds. Several modifier single mutants generate pointed leaves in common. Mutations in FASCIATA2 (FAS2) also cause pointed leaves. The FAS2 gene encodes a component of Chromatin Assembly Factor-1 (CAF-1), a histone chaperone, which affects mRNA levels of various genes through the regulation of chromatin states. In the present study, we demonstrate that as2 fas2 double mutants frequently generate abaxialized filamentous leaves and show increased mRNA levels of genes for leaf abaxialization, KANADI1 (KAN1), KAN2, YABBY5 (YAB5), ETTIN/AUXIN RESPONSE FACTOR3 (ETT/ARF3) and ARF4. In addition, the transcript levels of all four class 1 KNOTTED1-like homeobox genes that are involved in the maintenance of shoot apical meristem and the Kip-related Protein2 (KRP2) and KRP5 genes that are involved in the cell cycle progression are elevated in the as2 fas2. The mRNA levels of all genes other than YAB5, whose transcript levels rise in as2 fas2, were increased in the fas2 single mutants. Our data suggest that FAS2 participates in the establishment of leaf adaxial-abaxial polarity through the repression of the transcript levels of these genes on the as2 background.

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Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana

Cited by 23 publications

References 44 publications

Characterization of an A-type cyclin-dependent kinase gene from Dendrobium candidum

Characterization of an A-type cyclin-dependent kinase gene from Dendrobium candidum

The jasmonate-responsive GTR1 transporter is required for gibberellin-mediated stamen development in Arabidopsis

ASYMMETRIC LEAVES2 and FASCIATA2 cooperatively regulate the formation of leaf adaxial-abaxial polarity in Arabidopsis thaliana

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