Quantitative analysis viaSurface Enhanced Raman Scattering from Ag nano-colloids utilizing an oscillating cell and right-angle collection geometry

Abstract

We examine the application of an oscillating cell in combination with right-angle Raman scattered light collection geometry for quantitative Surface Enhanced (Resonance) Raman Scattering (SER(R)S) measurements from nano-colloidal noble metal solutions. This excitation/collection Raman configuration allows specific SERS and SERRS signatures of aqueous solutions of mitoxantrone, an antitumor drug, to be easily resolved at (sub)-ng/mL and (sub)-pg/mL concentration levels. A partial least-squares (PLS) chemometric algorithm was applied to predict the concentration of 25 microL of aqueous solutions of mitoxantrone added in 0.5 mL of a silver colloidal solution in a test tube attached to the oscillating cell. For SERS (514.5 nm) measurements, this was performed over the range from 0 to 13 ng/mL with a correlation coefficient R(2) of 98.5% and RMS error of prediction equal to 0.5 ng/mL. SERRS (632.8 nm) measurements performed over a range of 0 to 7 pg/mL gave R(2) = 98.92% and RMSE = 0.2 pg/mL.

Multiplexed concentration quantification using isotopic surface‐enhanced resonance Raman scattering

“…In spite of the difficulties associated with surface‐enhanced scattering variability, significant progress has been made in using SERS for quantitative concentration measurements 6, 10–13. These studies demonstrate the feasibility of using various substrates and sample handling strategies to improve the performance of SERS/SERRS in quantifying the concentrations of drugs, metabolites, and other compounds.…”

Mitoxantrone removal by electrochemical method: A comparison of homogenous and heterogenous catalytic reactions

“…All experiments were carried out in deionized water. An aqueous solution of MXT is blue and absorbs visible light at a wavelength of 630 nm (30). Hence, by determining its absorbance through the use of ultra violet-visible (UV-Vis) technique, it would be possible to determine its concentration in water using a calibration curve.…”

Evaluation of the release characteristics of covalently attached or electrostatically bound biocidal polymers utilizing SERS and UV-Vis absorption

“…Reproducible and improved Raman scattering collection measurements were allowed by the use of an 'oscillating cell' in combination with the advantage of utilizing the right angle light scattering collection geometry. A detailed description of this configuration has been reported before [16]. Reference FTRaman spectra were also obtained with NIR excitation at 1064 nm (via an R510 diode pumped Nd:YAG laser) utilizing a Raman component (FRA-106/S) attached to an Equinox 55 Bruker spectrometer.…”

“…The colloids used to collect the SERS spectra were prepared according to a modified Lee and Meisel procedure [16,20]. For these measurements, 100 μL aliquots were removed from the vials containing the piece of film immersed in ethanol or NaCl solution, but this aliquot volume was not returned or replaced.…”

“…SERS constitutes a challenge of applying it to extremely low concentration level analysis. Applying a surface enhanced Raman scattering excitation/collection configuration bearing an oscillating cell and combined with right angle scattering collection geometry we have quite recently introduced a new method to quantitatively monitor the level of active agents at very low concentration range [16,17]. The present work is mainly an attempt to monitor by SERS the release of biocide polymers from polymeric films and compare the release rate obtained with a traditional and consequently most commonly used technique in quantitative analysis of controlled release, such as UV-Vis absorption.…”