Quantitative analysis of zinc in prostate cancer tissues using synchrotron radiation microbeams

Ide‐Ektessabi, Ari; Fujisawa, Shigeyoshi; Sugimura, Kazuro; Kitamura, Yuko; Gotoh, Akinobu

doi:10.1002/xrs.532

Cited by 40 publications

(26 citation statements)

References 9 publications

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“…Besides that, specialized Zn uptake transporters in prostate epithelial cells were found (Beck et al 2004;Franklin et al 2005;Desouki et al 2007). However, Zn was found not only in prostatic glandular epithelium but also in the stromal component (Ide-Ektessabi et al 2002). Thus, the long-standing questions about the main pool and the local distribution of Zn in prostate tissue still remain open (Mawson and Fischer 1952;Hoare et al 1956;Siegal et al 1961;Kar and Chowdhury 1968;Dhar et al 1973;Morita 1981;Leake et al 1983;Tvedt et al 1989;Bataineh et al 2002;Franklin et al 2005).…”

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confidence: 99%

Age-related histological and zinc content changes in adult nonhyperplastic prostate glands

Zaichick

2013

AGE

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To clarify age-related histological and Zn content changes in nonhyperplastic adult prostate glands, a quantitative morphometric and energy-dispersive X-ray fluorescence analyses were performed. The prostates were obtained from autopsies of 63 subjects aged 21-70 years who died mainly from trauma. It was found that histologically normal prostate tissue undergoes substantial changes throughout aging. These changes are reflected in an increase of the percent volume of the glandular lumen for the third to fifth decades, reaching a maximum for the decade 41-50 years. Over the same period, the percent volume of the stroma remains steady, but the percent volume of epithelium decreases, approximately, linearly with age. The percent volume of glandular lumen (reflects the volume of prostatic fluid) in the prostate gland of men aged 41 to 50 years is 1.5-fold higher than that in men aged 21 to 30 years, but the epithelium/lumen (prostatic fluid) ratio is approximately twofold lower. This suggests that accumulation of the prostatic fluid develops from 30 to 50 years of age. This accumulation of the prostatic fluid results in an increase of the Zn mass fraction in the prostate. In turn, when the intraprostatic Zn level exceeds a certain level by the end of the fifth decade, it begins to work as a trigger for different factors, all of which increase the proliferation of stromal cells. Deductions from these results allow possible partial explanations of both relevant prostatic aging mechanisms and the effects of dietary interventions using supplementary Zn.

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confidence: 99%

Age-related histological and zinc content changes in adult nonhyperplastic prostate glands

Zaichick

2013

AGE

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“…For example, the concentration of Zn was studied in prostate cancer tissues [75], and was found to be It seems that there is no clear evidence of significant differences between cancerous and normal tissues. However, more recently, clearer results were obtained for colorectal liver cancer, which showed relevant differences, particularly for zinc and iron (Fig.…”

Section: Medical Sciencesmentioning

confidence: 99%

X-Ray Fluorescence Spectrometry

Cesareo

2010

Ullmann's Encyclopedia of Industrial Chemistry

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“…This technology can not only determine trace element quality and quantity but can also detect combined elements and oxidation state. The trace elements, including Zn [8], Ca, Mn, and Fe [9], are typically studied using synchrotron radiation [10]. Currently, the mechanism of action of organic zinc (as well as organic trace element) is, at present, a "black box".…”

Section: Introductionmentioning

confidence: 99%

Study on Zn Relative Concentration and State in Sheep Duodenum by XAFS

Sui

Liu

Ren

et al. 2010

Biol Trace Elem Res

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Synchrotron-based X-ray absorption fine structure (XAFS) spectroscopy is not widely used in animal science. The objective of this study was to employ the XAFS technique to determine changes in zinc absorption and concentrations in the sheep interstinal sac using different zinc sources. Forty-eight sheep were slaughtered and their duodena were dissected. The duodena were randomly assigned to six zinc sources (ZnO, ZnSO(4), ZnMet, ZnLys, ZnSO(4) + methionine, ZnSO(4) + lysine). Ten centimeters of duodenal sac midpiece was incubated for 40 min in vitro using the everted intestinal sac technique in culture medium containing zinc from different sources. The amount of zinc present was normalized to 4 mg. XAFS was used to analyze the relative concentration and oxidation state of zinc, and atomic absorption spectrometry (AAS) was used to verify zinc concentration. The results showed that, for increasing zinc concentrations, organic zinc was a better source than inorganic zinc. In addition, the zinc concentration achieved using ZnMet was higher than that for ZnO and ZnSO(4) (P < 0.05) as measured by atomic absorption spectroscopy. The results using XAFS were consistent with that of AAS. The states of organic zinc and inorganic zinc were identical after being incubated for 40 min. As observed in these experiments, organic zinc was more easily absorbed than inorganic zinc. Our data demonstrate that organic zinc was dissociated into ions and then absorbed as inorganic zinc. In our experiments, we are the first investigators to use XAFS spectroscopy to determine zinc absorption in the sheep duodenal wall. We observed reduced absorption of inorganic zinc in the presence of methionine or lysine. Taken together, we postulate that the optimal molar ratio of inorganic zinc and ligand requires further study.

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Quantitative analysis of zinc in prostate cancer tissues using synchrotron radiation microbeams

Cited by 40 publications

References 9 publications

Age-related histological and zinc content changes in adult nonhyperplastic prostate glands

Age-related histological and zinc content changes in adult nonhyperplastic prostate glands

X-Ray Fluorescence Spectrometry

Study on Zn Relative Concentration and State in Sheep Duodenum by XAFS

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