Quantitative Analysis of Various B-ring Unsubstituted and Substituted Flavonoids in Ten Australian Species of <i>Eucalyptus</i>

Saraf, Isha; Marsh, Karen J.; Vir, Sanjay; Foley, William J.; Singh, Inder Pal

doi:10.1177/1934578x1701201109

Cited by 6 publications

(5 citation statements)

References 15 publications

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“…This fragment ion further lost deoxyhexose to form the base peak [M−H−C 10 H 14 O 2 −C 6 H 10 O 4 ] − at m / z 301 (quercetin). Combining the fragmentation pattern of the compound with literature data [ 15 , 16 ], it was inferred that the compound was Eucalmaidin D/cypellogin A/B, and its fragmentation pattern is shown in Figure 6 .…”

Section: Resultsmentioning

confidence: 78%

“…Based on the above information and literature [14], it was inferred that compound 13 is Sideroxylonal A/B/C, and its fragmentation pattern is shown in Figure 5a. Combining the fragmentation pattern of the compound with literature data [15,16], it was inferred that the compound was Eucalmaidin D/cypellogin A/B, and its fragmentation pattern is shown in Figure 6.…”

Section: Phloroglucinolsmentioning

confidence: 80%

mentioning

confidence: 93%

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Preliminary Study on Total Component Analysis and In Vitro Antitumor Activity of Eucalyptus Leaf Residues

Wu,

Wang,

Cheng

et al. 2024

Molecules

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Eucalyptus globulus is widely introduced and cultivated in Yunnan province. Its foliage is mainly used to extract eucalyptus oil, but the by-product eucalyptus residue has not been fully utilized. Based on the above reasons, in this study, we sought to explore the comprehensive utilization potential of eucalyptus resources. The total composition of eucalyptus residue was analyzed by ultra performance liquid chromatography-time-of-flight mass spectrometry (UPLC-Q/TOF MS), and the active components and nutrient components of eucalyptus leaf residue were determined by chemical methods and liquid phase techniques. Meanwhile, the antitumor activity of triterpenoids in eucalyptus leaves was evaluated by tetramethylazazole blue colorimetric assay (MTT). The results of qualitative analysis indicated that 55 compounds were identified from eucalyptus residue, including 28 phloroglucinols, 17 terpenoids, 3 flavonoids, 5 fatty acids, 1 amino acid and 2 polyphenols. Among them, the pentacyclic triterpenoids, in eucalyptus residue, were mainly oleanane type and urthane type. The results of quantitative determination indicated that the content of triterpenoid compounds was 2.84% in eucalyptus residue, which could be enhanced to 82% by silicone separation. The antitumor activity results showed that triterpenoid compounds have moderate inhibitory effects on human breast cancer cell MDA-MB-231, gastric adenocarcinoma cell SGC-7901 and cervical cancer cell Hela. The half maximal inhibitory concentration (IC50) was 50.67, 43.12 and 42.65 μg/mL, respectively. In this study, the triterpenoids from eucalyptus leaf residues were analyzed to reveal that the triterpenoids from eucalyptus leaf have antitumor effects and have potential to be developed as antitumor drugs.

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Section: Resultsmentioning

confidence: 78%

Section: Phloroglucinolsmentioning

confidence: 80%

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Preliminary Study on Total Component Analysis and In Vitro Antitumor Activity of Eucalyptus Leaf Residues

Wu,

Wang,

Cheng

et al. 2024

Molecules

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“…No reports are available on the antioxidant activity of E. rossii , nor E. cupressiformis , as far as the authors are aware. Similarly, there is limited literature on the phytochemistry of these species—Saraf et al [ 14 ] reported four flavonoids in E. rossii , and Cook and Haynes [ 15 ] reported four flavonoid glycosides in E. cupressiformis .…”

Section: Introductionmentioning

confidence: 99%

Multi-Dimensional Antioxidant Screening of Selected Australian Native Plants and Putative Annotation of Active Compounds

et al. 2023

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Acacia implexa, Eucalyptus rossii and Exocarpos cupressiformis are native plants of Australia, which were used by the First Peoples for medicinal purposes. In this study, 70% aqueous ethanol crude extracts were prepared from A. implexa bark and leaves, E. rossii leaves and E. cupressiformis leaves, and partitioned via sequential extraction with n-hexane, dichloromethane (DCM), ethyl acetate and ethanol. The crude extracts and fractions were screened for antioxidant activity using a novel, high-throughput lipid-based antioxidant assay, as well as the aqueous ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) assay and the Folin–Ciocalteu test for total phenols. In the lipid-based assay, non-polar n-hexane and DCM fractions showed higher antioxidant activity against the formation of peroxides and thiobarbituric acid reactive substances (TBARS) than the other fractions, whereas the non-polar fractions were not effective in aqueous assays. This illustrates that the high potential of the lipid-soluble n-hexane and DCM fractions as antioxidants would have been missed if only aqueous-based assays were used. In addition, the potent antioxidant compounds were putatively annotated using liquid chromatography quadrupole time-of-flight mass spectrometry (LC-qTOF-MS). Gallic acid, (+)-catechin, (−)-epicatechin and tannins were found in most crude extracts.

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“…Brushtail possums were offered a formulated diet with or without the PSM, flavanone. Flavanone is a representative of the unsubstituted B-ring flavanone (UBF) class of PSMs present in the foliage of many eucalypt species (Saraf et al, 2017;Marsh et al, 2019), and is known to deter brushtail possums from feeding (Marsh et al, 2015). One week before feeding experiments to reduce any effects of novelty, brushtail possums were offered the basal diet containing 0.625% DM flavanone (Sigma-Aldrich, Sydney, NSW, Australia) on one night, and 1.25% DM flavanone the next night.…”

Section: Dietsmentioning

confidence: 99%

Warmer Ambient Temperatures Depress Detoxification and Food Intake by Marsupial Folivores

Beale

Connors²,

Dearing³

et al. 2022

Front. Ecol. Evol.

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Ambient temperature is an underappreciated determinant of foraging behaviour in wild endotherms, and the requirement to thermoregulate likely influences food intake through multiple interacting mechanisms. We investigated relationships between ambient temperature and hepatic detoxification capacity in two herbivorous marsupials, the common ringtail possum (Pseudocheirus peregrinus) and common brushtail possum (Trichosurus vulpecula) that regularly feed on diets rich in plant toxins. As an indicator of hepatic detoxification capacity, we determined the functional clearance rate of an anaesthetic agent, Alfaxalone, after possums were acclimated to 10°C [below the thermoneutral zone (TNZ)], 18°C [approximately lower critical temperature (LCT)], and 26°C [approximately upper critical temperature (UCT)] for either 7 days or less than 24 h. We then measured intake of foods with high or low plant secondary metabolite (PSM) concentrations under the same temperature regimes. After 7 days of acclimation, we found a positive correlation between the functional clearance rate of Alfaxalone and ambient temperature, and a negative relationship between ambient temperature and intake of foods with high or low PSM concentrations for both species. The effect of ambient temperature on intake of diets rich in PSMs was absent or reduced when possums were kept at temperatures for less than 24 h. Our results underscore the effects of ambient temperature in hepatic metabolism particularly with respect intake of diets containing PSMs. Given that the planet is warming, it is vital that effects of ambient temperature on metabolism, nutrition and foraging by mammalian herbivores is taken into account to predict range changes of species and their impact on ecosystems.

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Quantitative Analysis of Various B-ring Unsubstituted and Substituted Flavonoids in Ten Australian Species of Eucalyptus

Cited by 6 publications

References 15 publications

Preliminary Study on Total Component Analysis and In Vitro Antitumor Activity of Eucalyptus Leaf Residues

Preliminary Study on Total Component Analysis and In Vitro Antitumor Activity of Eucalyptus Leaf Residues

Multi-Dimensional Antioxidant Screening of Selected Australian Native Plants and Putative Annotation of Active Compounds

Warmer Ambient Temperatures Depress Detoxification and Food Intake by Marsupial Folivores

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