Quantitative Analysis of Vaccine Antigen Adsorption to Aluminum Adjuvant Using an Automated High-Throughput Method

Ahl, Patrick L.; Wang, Sheng-ching; Chintala, Ramesh; Mensch, Christopher; Smith, William J.; Wenger, Marc; Blue, Jeffrey T.

doi:10.5731/pdajpst.2017.008250

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…This process is rather laborious, but can be readily automated using high-throughput liquid handling systems. 46 – 48 The adsorption of protein antigens to aluminum adjuvants has been typically analyzed according to the Langmuir adsorption model, which allows calculation of several adsorptive parameters including the adsorptive coefficient, a correlate of the strength of the antigen−adjuvant interaction. 38 , 49 However, although the adsorption isotherm can resemble the Langmuir model, the adsorption of proteins to solid surfaces does not meet the premises on which the Langmuir model is based.…”

Section: Formulation Of Vaccines With Aluminum Adjuvantsmentioning

confidence: 99%

“…Isotonicity can be obtained by formulation with 150 mM NaCl, but NaCl can reduce the adsorption of electrostatically adsorbed antigens. 48 , 65 This has been attributed to shielding of electric charges resulting in decreased electrostatic interactions. In addition, increased aggregation of primary AH particles results in a decrease of the adsorptive surface area.…”

Section: Formulation Of Vaccines With Aluminum Adjuvantsmentioning

confidence: 99%

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Optimizing the utilization of aluminum adjuvants in vaccines: you might just get what you want

2018

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Aluminum-containing adjuvants have been used for over 90 years to enhance the immune response to vaccines. Recent work has significantly advanced our understanding of the physical, chemical, and biological properties of these adjuvants, offering key insights on underlying mechanisms. Given the long-term success of aluminum adjuvants, we believe that they should continue to represent the “gold standard” against which all new adjuvants should be compared. New vaccine candidates that require adjuvants to induce a protective immune responses should first be evaluated with aluminum adjuvants before other more experimental approaches are considered, since use of established adjuvants would facilitate both clinical development and the regulatory pathway. However, the continued use of aluminum adjuvants requires an appreciation of their complexities, in combination with access to the necessary expertise to optimize vaccine formulations. In this article, we will review the properties of aluminum adjuvants and highlight those elements that are critical to optimize vaccine performance. We will discuss how other components (excipients, TLR ligands, etc.) can affect the interaction between adjuvants and antigens, and impact the potency of vaccines. This review provides a resource and guide, which will ultimately contribute to the successful development of newer, more effective and safer vaccines.

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Section: Formulation Of Vaccines With Aluminum Adjuvantsmentioning

confidence: 99%

Section: Formulation Of Vaccines With Aluminum Adjuvantsmentioning

confidence: 99%

Optimizing the utilization of aluminum adjuvants in vaccines: you might just get what you want

2018

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Re-inventing traditional aluminum-based adjuvants: Insight into a century of advancements

Gogoi,

Mani,

Bhatnagar

2024

International Reviews of Immunology

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Established and advanced adjuvants in vaccines' formulation: Mineral adsorbents, nanoparticulate carriers and microneedle delivery systems

Krajišnik¹,

Ilić²,

Nikolić³

et al. 2019

Arhiv za farmaciju

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In the era of modern vaccinology, limited immunogenicity of the most commonly used antigens has enforced the use of various adjuvants in vaccine formulations to achieve desired immune response. Aluminum-containing adjuvants have been, historically, the most widely used mineral immunostimulants, generally regarded as safe to use in human vaccines. The great academic progress in inorganic (nano)materials synthesis, structure control and functionalization design has led to a growing interest in innovative adjuvants such as clays, mesoporous silica nanoparticles, zinc oxide, iron oxide and iron hydroxide nanoparticles, etc. On the other hand, there has been an intention to use specific nanoparticulated antigen delivery systems, such as nanoemulsions, in order to protect antigens from premature proteolytic degradation and/or to improve antigen immunogenicity by facilitating antigen uptake and processing by antigen presenting cells. Simultaneously, numerous research efforts have been focused on the development of innovative technologies for antigen delivery into the skin (such as microneedles), with the aim to improve vaccine efficacy alongside with enhanced patient adherence, particularly in children population (noninvasive or minimally invasive administration). Therefore, this review deals with each of these approaches in more detail, with the special emphasis on examples of their use in vaccine formulations as well as on the factors influencing their efficacy and safety.

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Quantitative Analysis of Vaccine Antigen Adsorption to Aluminum Adjuvant Using an Automated High-Throughput Method

Cited by 3 publications

References 29 publications

Optimizing the utilization of aluminum adjuvants in vaccines: you might just get what you want

Optimizing the utilization of aluminum adjuvants in vaccines: you might just get what you want

Re-inventing traditional aluminum-based adjuvants: Insight into a century of advancements

Established and advanced adjuvants in vaccines' formulation: Mineral adsorbents, nanoparticulate carriers and microneedle delivery systems

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