Quantitative analysis of urinary endogenous markers for the treatment effect of Radix Scutellariae on type 2 diabetes rats

Hou, Guangyue; Men, Lihui; Wang, Lu; Zheng, Zhong; Liu, Zhiqiang; Song, Fengrui; Liu, Zhongying

doi:10.1016/j.cclet.2016.12.039

Cited by 5 publications

(2 citation statements)

References 30 publications

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“… 33 Since the collection of urine samples was convenient and non-invasive, monitoring the metabolic end products in urine was practical and efficient in clinical application. A wealth of metabolic studies were carried out to investigate the alteration of metabolites in urine samples 34 , 35 under diabetic conditions. Among the altered metabolites, urinary NAGlys are the primary class of metabolites in the detoxification system of the human body.…”

Section: Resultsmentioning

confidence: 99%

Derivatization of N-Acyl Glycines by 3-Nitrophenylhydrazine for Targeted Metabolomics Analysis and Their Application to the Study of Diabetes Progression in Mice

Shi

et al. 2023

Anal. Chem.

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N-Acyl glycines (NAGlys) are an important class of metabolites in the detoxification system of the human body. They have been used in the diagnosis of several metabolic diseases. Liquid chromatography−mass spectrometry (LC−MS) is the most frequently used NAGlys detection platform. Here, we describe a simple and sensitive method of NAGlys detection by LC−MS in plasma and urine samples. This approach is based on the use of a derivatization reagent, 3-nitrophenylhydrazine. The reaction is quick in aqueous solution, and no quenching step is needed. To expand the coverage of NAGlys when standards are not available, NAGlys were first identified based on high-resolution LC−MS. Quantification was subsequently carried out on triple quadrupole LC−MS. This approach allowed a much broader measurement of NAGlys (41 NAGlys in total), especially when authentic standards are unavailable. Comprehensive analysis of NAGlys with this new method was applied in plasma and urine samples of db/db diabetic and non-diabetic db/m+ control mice. The majority of detected NAGlys were altered with high differentiation ability in plasma and urine samples from diabetic and non-diabetic mice. These identified NAGlys hold the potential to be diagnostic biomarkers for type II diabetes and diabetic complications.

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Section: Resultsmentioning

confidence: 99%

Derivatization of N-Acyl Glycines by 3-Nitrophenylhydrazine for Targeted Metabolomics Analysis and Their Application to the Study of Diabetes Progression in Mice

Shi

et al. 2023

Anal. Chem.

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“…The intricate and multiple daily injection is prone to decrease the patient's compliance. Furthermore, the risk of hypoglycemia owing to frequent mealtime-related administration was significantly improved 78 .…”

Section: Applications Of Microneedlesmentioning

confidence: 99%

Recent advances of microneedles for biomedical applications: drug delivery and beyond

Yang

Liu

et al. 2019

Acta Pharmaceutica Sinica B

258

175

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The microneedle (MN), a highly efficient and versatile device, has attracted extensive scientific and industrial interests in the past decades due to prominent properties including painless penetration, low cost, excellent therapeutic efficacy, and relative safety. The robust microneedle enabling transdermal delivery has a paramount potential to create advanced functional devices with superior nature for biomedical applications. In this review, a great effort has been made to summarize the advance of microneedles including their materials and latest fabrication method, such as three-dimensional printing (3DP). Importantly, a variety of representative biomedical applications of microneedles such as disease treatment, immunobiological administration, disease diagnosis and cosmetic field, are highlighted in detail. At last, conclusions and future perspectives for development of advanced microneedles in biomedical fields have been discussed systematically. Taken together, as an emerging tool, microneedles have showed profound promise for biomedical applications.

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Future perspective, clinical translation, commercial challenges, and industrial success with microneedle-based products

Kaur,

Kar,

Gupta

et al. 2024

Design and Applications of Microneedles in Drug Delivery and Therapeutics

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Quantitative analysis of urinary endogenous markers for the treatment effect of Radix Scutellariae on type 2 diabetes rats

Cited by 5 publications

References 30 publications

Derivatization of N-Acyl Glycines by 3-Nitrophenylhydrazine for Targeted Metabolomics Analysis and Their Application to the Study of Diabetes Progression in Mice

Derivatization of N-Acyl Glycines by 3-Nitrophenylhydrazine for Targeted Metabolomics Analysis and Their Application to the Study of Diabetes Progression in Mice

Recent advances of microneedles for biomedical applications: drug delivery and beyond

Future perspective, clinical translation, commercial challenges, and industrial success with microneedle-based products

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