Quantitative analysis of underivatized amino acids in the sub- to several-nanomolar range by ion-pair HPLC using a corona-charged aerosol detector (HPLC–CAD)

Furota, Satoshi; Ogawa, Nanako O.; Takano, Yoshinori; Yoshimura, Toshihiro; Ohkouchi, Naohiko

doi:10.1016/j.jchromb.2018.07.033

Cited by 29 publications

(11 citation statements)

References 36 publications

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“…11,13 Of these, ion-pair HPLC has proven a useful method for the isolation of underivatized amino acids. 10,13,14 However, procedural blanks stemming from HPLC separation suggest that a non-negligible amount of carbon contamination is contained in the isolated fractions, 3,11 the latter being derived from column bleed, solvents, and coelution of undesired compounds. 15 Recent developments in accelerator mass spectrometry (AMS) have drastically reduced sample sizes (<100 μgC) required for radiocarbon (Δ 14 C) measurement.…”

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Improved Method for Isolation and Purification of Underivatized Amino Acids for Radiocarbon Analysis

et al. 2018

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We have improved a method for isolation and purification of individual amino acids for compound-specific radiocarbon analysis (CSRA). To remove high-performance liquid chromatography (HPLC) eluent blanks from isolated amino acid fractions prior to the radiocarbon (ΔC) measurement, each fraction was filtered through a membrane filter and then washed with diethyl ether twice. Radiocarbon measurements on standard amino acids processed and purified with the above method using elemental analyzer-accelerator mass spectrometry resulted in ΔC values that were in strong agreement ( R = 0.998) with the original ΔC value of each amino acid standard. From these measurements, we calculate dead and modern carbon contamination contributions as 1.2 ± 0.2 and 0.3 ± 0.1 μgC, respectively, which are consistent with direct assessments of HPLC procedural blanks of 1.0 ± 0.8 μgC per sample. These contamination constraints allow correction of measured ΔC values for accurate and precise CSRA and are widely applicable to future archeological and biogeochemical studies.

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Improved Method for Isolation and Purification of Underivatized Amino Acids for Radiocarbon Analysis

et al. 2018

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“…Underivatised amino acids were separated by reverse-phase high-performance liquid chromatography (HPLC) using an Agilent 1260 Infinity HPLC system equipped with an Agilent Poroshell 120 EC-C18 column (4.6 × 150 mm, 2.7 µm). Using a flow rate of 0.6 mL/min and an injection volume of 7 µl, analyte peaks were detected with a Corona charged aerosol detector (CAD; Corona CAD veo; Thermo Fisher Scientific Inc.) and eluted using two mobile phases (Solvent A: 0.4% heptafluorobutyric acid and 0.02% trifluoroacetic acid (TFA) in distilled water, Solvent B: 0.1% TFA in acetonitrile, modified from Furota, et al 46 . Amino acid standards (0, 0.125 and 2 µmol 1 -1 ) containing 16 amino acids were used to calibrate the analysis; arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine and valine are essential (i.e.…”

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Increased insect herbivore performance under elevated CO2 is associated with lower plant defence signalling and minimal declines in nutritional quality

Johnson

Waterman

Hall

2020

Sci Rep

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changes in insect herbivore performance under elevated atmosphere carbon dioxide concentrations e[co 2 ] are often driven by changes in the nutritional and defensive chemistry of their host plants. Studies addressing how the prolific pest cotton bollworm (Helicoverpa armigera) responds to e[co 2 ] show that performance usually declines, often associated with lower nutritional (e.g. nitrogen (N) concentrations) quality of host plants under e[CO 2 ]. We investigated the impacts of e[co 2 ] on nutritional quality and anti-herbivore (jasmonate) defensive signalling in lucerne (Medicago sativa) when challenged by H. armigera. While foliar n decreased under e[co 2 ], other aspects of nutritional quality (soluble protein, amino acids, foliar C:N) were largely unaffected, potentially due to increased root nodulation under e[co 2 ]. in contrast, e[co 2 ] greatly reduced jasmonate signalling in M. sativa following H. armigera attack; jasmonic acid concentrations were ca. 56% lower in attacked plants grown under e[co 2 ]. concurrent with this, relative growth rates of H. armigera were ca. 66% higher when feeding on e[co 2 ]-grown plants. in contrast with previous reports, which we meta-analytically summarise, we provide the first evidence that H. armigera performance can increase under e[co 2 ]. this may occur in plants, such as M. sativa, where e[co 2 ] has limited impacts on nutritional quality yet reduces jasmonate defence signalling. With global populations expected to reach 11.2 billion by 2,100 there is an urgent need to ensure future food security, a challenge which is complicated by global climate change 1. Invertebrate pests destroy enough food to feed a billion people a year 2 which has, in part, fuelled interest in understanding which pests may become more problematic under predicted changes in the Earth's climate 3. In particular, unprecedented increases in atmospheric carbon dioxide (CO 2) have the capacity to change plant chemistry which affect the susceptibility of crops to insect herbivores 4-6. The effects of elevated atmospheric CO 2 concentration (e[CO 2 ]) on plant nutritional and defensive chemistry, and their consequent effects on invertebrate herbivores, have received extensive attention 7-9. In terms of nutritional quality, nitrogen availability is considered to be the limiting factor in insect herbivore diets 10. Broadly speaking, e[CO 2 ] causes foliar nitrogen (N) concentrations to decrease due to one or more processes, including dilution effects (i.e. relative to increased carbohydrate concentrations), reduced N uptake by roots, increased NH 3 volatilization and reduced investment in the N-rich enzyme RUBISCO 11-14. The impacts of e[CO 2 ] on plant defences are less easily predicted, but some trends are emerging 5. Plant defences against herbivorous arthropods are regulated by several phytohormonal pathways, including the jasmonic acid (JA), salicylic acid (SA) and ethylene signalling pathways 15,16. Of these, the JA pathway is regarded as the master regulator of plant resistance to arthropod her...

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“…S1). These peaks should be incombustible inorganic ions because the carbon and nitrogen of these peaks were not detected in the EA analysis (Furota et al 2018). The fractions of Glu (Fraction ii, retention time 30.0–32.0 min) and Phe (Fraction v, retention time 50.0–52.0 min) were collected as single target compounds (Fig.…”

Section: Assessmentmentioning

confidence: 99%

Integrative assessment of amino acid nitrogen isotopic composition in biological tissue samples determined by GC/C/IRMS, LC × EA/IRMS, and LC × GC/C/IRMS

Ishikawa

Ogawa

Sun

et al. 2022

Limnology & Ocean Methods

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Compound-specific isotope analysis of nitrogen (δ 15 N) in amino acids (CSIA-AA) has significantly contributed to environmental sciences such as anthropology, biogeochemistry, and ecology. Several methods exist for determining δ 15 N of amino acids (AAs). Although these methods have their own strength and weakness, they have not been intercalibrated yet, especially for biological samples with matrices. To address this issue, we systematically compared AA δ 15 N values among three methods using gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS), preparative liquid chromatography (LC) separation followed by elemental analyzer/ IRMS (LC Â EA/IRMS), and LC separation followed by GC/C/IRMS (LC Â GC/C/IRMS). The δ 15 N values of glutamic acid (δ 15 N Glu ) and phenylalanine (δ 15 N Phe ) in fish muscle, two crucial AAs for estimating the trophic positions (TPs) of organisms, were compared among methods. Although a significant difference in fish muscle δ 15 N Glu values was found among the three analytical methods, their δ 15 N Glu and δ 15 N Phe values were fairly consistent between all pairs of methods (n = 8, R 2 = 0.9968 for GC/C/IRMS vs. LC Â GC/C/IRMS; 0.9936 for LC Â EA/IRMS vs. LC Â GC/C/IRMS; and 0.9912 for GC/C/IRMS vs. LC Â EA/IRMS), which resulted in similar TP estimates among the methods. Thus, the results provide empirical validation that the CSIA-AA is comparable among different methods in interdisciplinary research fields. We also highlighted some critical features of each of the three analytical methods that can be used as a guideline for future CSIA-AA research.

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Quantitative analysis of underivatized amino acids in the sub- to several-nanomolar range by ion-pair HPLC using a corona-charged aerosol detector (HPLC–CAD)

Cited by 29 publications

References 36 publications

Improved Method for Isolation and Purification of Underivatized Amino Acids for Radiocarbon Analysis

Improved Method for Isolation and Purification of Underivatized Amino Acids for Radiocarbon Analysis

Increased insect herbivore performance under elevated CO2 is associated with lower plant defence signalling and minimal declines in nutritional quality

Integrative assessment of amino acid nitrogen isotopic composition in biological tissue samples determined by GC/C/IRMS, LC × EA/IRMS, and LC × GC/C/IRMS

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