Quantitative analysis of triterpene lupeol and anti-inflammatory potential of the extracts of traditional pain-relieving medicinal plants Derris scandens, Albizia procera, and Diospyros rhodocalyx

Abstract

A BSTRACT Derris scandens , Albizia procera , and Diospyros rhodocalyx have traditionally been used as herbal remedies for pain relief in Thailand. The ethanolic extracts of these plants obtained by Soxhlet extraction were analyzed by the developed high-performance liquid chromatography-diode-array detection method. Lupeol, the anti-inflammatory triterpene, was selected as a chemical marker for this investigation. All extracts together with that compound were further evaluated for their potential on anti-inflammatory activity using 5-lipoxygenase inhibition assay. Lupeol in each extract was quantified and expressed in the range of 21.44 ± 0.89–40.72 ± 0.40 mg per 100 g of crude drug and the enzyme inhibitory activity of all tested extracts presented as half-maximal inhibitory concentration values ranged between 63.71 ± 2.09 and 91.09 ± 1.40 μg/mL. This study shows that the developed analytical method is effective for analyzing triterpene lupeol in these plants and also reveals the relationship between a lupeol content and the anti-inflammatory effect.

Combination of selected Thai traditional pain relief medicinal plants with anti-inflammatory abilities in a protein denaturation assay

“…The analytical method employed in this study was based on that proposed by Somwong and Theanphong (2021). Prior to performing the analysis, the analytical procedure was verified.…”

“…As a basis, the compound was chosen as a chemical marker in this investigation. Our previous research on the anti-inflammatory effects of Thai herbal pain relievers led us to hypothesize that the active extracts of the plants that were subjected to this study might contain lupeol (Somwong and Theanphong 2021). Thus, chemical analysis was carried out in this work, utilizing the Soxhlet apparatus and HPLC-DAD to extract and measure the lupeol content of plant samples, respectively.…”

Wound-healing activity and quantification of bioactive compounds from Derris scandens extract

“…By following a process detailed in a previous publication, the acquired sample was authenticated using a genuine specimen deposited at the Department of Pharmacognosy, College of Pharmacy, Rangsit University, Thailand (RSU 0089). [ 7 ] Genistein (≥98% purity) was provided by Sigma-Aldrich, Singapore, and lupeol (99% purity) was supplied by Nanjing Spring and Autumn Biological Engineering Co., Ltd., China. All chemical reagents and disposable HPLC instrument accessories were provided by Merck KGaA, Germany, and S.N.P.…”

“…The analysis was carried out using an isocratic elution of the mobile phase with a flow rate of 1.0 mL/min of 90:10 methanol and acetonitrile, and a DAD at 210 nm wavelength, as described in our earlier research. [ 7 ] The protocol used in the quantitative genistein study was applied to examine lupeol contents, as well as to validate the analytical method and investigate the statistical differences.…”

“…[ 2 ] It demonstrated a high cytotoxic effect against colon adenocarcinoma (SW620) cell lines, with an IC 50 of 30.50 μg/mL,[ 6 ] and a specific anti-inflammatory effect in the 5-LOX enzyme test, with an IC 50 of 69.26 μg/mL. [ 7 ] With this in mind, these compounds were chosen as chemical markers in the current study, and chemical analysis was performed using Soxhlet extraction and HPLC to examine the contents of these compounds in the ethanolic extracts of DS stems, which were evaluated for their potential on in vitro cytotoxicity and wound-healing activity toward skin fibroblast cells that had not previously been investigated. The purpose of the study was to explore the wound-healing properties of DS extracts for use in skin treatments, as well as the phytochemical components in the extracts that were responsible for the activity.…”