Quantitative Analysis of Tissue Secretome Reveals the Diagnostic and Prognostic Value of Carbonic Anhydrase II in Hepatocellular Carcinoma

Abstract

6Early detection and intervention are key strategies to reduce mortality, increase 3 7 long-term survival and improve the therapeutic effects of hepatocellular carcinoma 3 8 (HCC) patients. Herein, the isobaric tag for relative and absolute quantitation (iTRAQ) 3 9 quantitative proteomic strategy was used to study the secretome in conditioned media 4 0 from HCC cancerous tissues, surrounding noncancerous and distal noncancerous 4 1 tissues to identify diagnostic and prognostic biomarkers for HCC. In total, 22 and 49 4 2 secretory proteins were identified to be dysregulated in the cancerous and surrounding 4 3 noncancerous tissues compared with the distal noncancerous tissues. Among these 4 4proteins, carbonic anhydrase II (CA2) was identified to be significantly upregulated in 4 5