Quantitative analysis of the profiles of twelve major compounds in Gentiana straminea Maxim. Roots by LC-MS/MS in an extensive germplasm survey in the Qinghai-Tibetan plateau

An integrated strategy for quality control of the multi-origins herb medicine of Gentianae Macrophyllae Radix based on UPLC-Orbitrap-MS/MS and HPLC-DAD

“…It is very important to select the authentic and high-quality medicinal materials because the level of the compounds is directly related to the clinical efficacy. 6,7,14,15,24,25…”

“…It is very important to select the authentic and high-quality medicinal materials because the level of the compounds is directly related to the clinical efficacy. 6,7,14,15,24,25 In conclusion, the developed LC-Orbitrap-MS and HPLC strategy is of great importance for quality control and authentication of Gentianae Macrophyllae Radix. The further study is needed for the comparison of pharmacological effects of Gentianae Macrophyllae Radix of the four species and the impact of geographical and ecological environment on its chemicals.…”

“…1). 1,[5][6][7] Numerous studies have proved that this herbal medicine is abundant in iridoids and secoiridoids, such as loganic acid and gentiopicroside, which have been recorded as quality markers in the Chinese Pharmacopoeia (2020 edition). 3,8 Loganic acid, 6 ′ -O-b-D-glucosylgentiopicroside, swertiamarine, gentiopicroside, and sweroside are reported to have various excellent activities.…”

Enhanced Identification of Ginsenosides Simultaneously from Seven Panax Herbal Extracts by Data-Dependent Acquisition Including a Preferred Precursor Ions List Derived from an In-House Programmed Virtual Library

“…It is crucial to establish precise and fit-for-purpose analytical techniques that can implement the characterization and quantification of metabolites for a number of research areas (e.g., natural product research and food science). , At the same time, it is still confronted with challenges for the detection and analysis of the metabolites owing to the severe endogenous interference and, in most cases, the low content in a complex matrix. In recent years, liquid chromatography/mass spectrometry (LC/MS) has provided a powerful and practical method enabling the systematic characterization of known compounds, as well as the discovery of potentially new components from natural products. − Nowadays, the utilization of multidimensional chromatography (MDC) greatly improves the selectivity and expands the peak capacity, , while various MS strategies are available to maximize the qualitative MS information supporting the identification of metabolites. , The utilization of various scan methods has been demonstrated as potent in the in-depth characterization and multicomponent quantitation of various metabolites from herbal extracts. , …”

Effective Parts of Gentiana straminea Maxim Attenuates Hypoxia-Induced Oxidative Stress and Apoptosis

“…16 It is a traditional Tibetan medicine with a history of more than 2000 years. 17 Pharmacological studies indicated that G.s Maxim mediates antipyretic, analgesic, anti-inflammatory, and antihypertensive effects; it also participates in central sedation, hepatoprotective, bile secretion, and immune regulation activities. [18][19][20][21] Our previous work demonstrated the protective effect of ethanol extracts from G.s Maxim on lung and heart damage in rats at high altitude.…”