Quantitative analysis of the phospholipids of some marine bioluminescent bacteria

Eberhard, Anatol; Rouser, George

doi:10.1007/bf02531378

Cited by 10 publications

(3 citation statements)

References 21 publications

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“…lipid found in G. oxydans with that reported from other gram-negative bacteria. The 7% free-lipid value found in the present study for early-exponential-phase Gluconobacter is well within the range of 5 to 14% reported for other gram-negative bacteria (2,11,15,21,22,30,31); however, the previously reported range may be too high. The majority ofprevious investigators used in vacuo drying to determine the percentage of lipid in the cell (2,15,21,22), yet our study demonstrates that in vacuo drying of replicate lipid extracts from the same cell batch yields more widely fluctuating individual values and a much higher average compared with other replicate extracts taken from the same cell batch and subjected to nitrogen drying.…”

Section: Discussionsupporting

confidence: 86%

“…The quantity found in G. oxydans compares favorably with that found in Pseudomonas aeruginosa (11) and Rhodomicrobium vanniellii (22), but most previous investigations of lipid quantity in gramnegative cells do not mention having determined the percentage of bound lipid (8,15,21,30,31). The present study and previous reports by others (11,22) suggest that bound lipid accounts for about 25% of the total lipid and 2% of the weight of exponentially growing, gram-negative cells. In contrast, the quantity of bound lipid in gram-positive cells usually forms a much greater proportion (17) or even the majority (14) of the total lipid of the cell.…”

Section: Discussionmentioning

confidence: 68%

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Change in quantity of lipids and cell size during intracytoplasmic membrane formation in Gluconobacter oxydans

Heefner¹,

Claus²

1976

J Bacteriol

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Electron microscopy previously revealed that Gluconobacter oxydans differentiates by forming quantities of intracytoplasmic membranes at the end of exponential growth. It was also shown that the formation of these membranes appears concurrently with an increased rate of polyol oxidation. In the present study, exponential-phase cells devoid of intracytoplasmic membranes were harvested and the quantity of free lipid was determined. This quantity was compared with that extracted from cells harvested 4 and 16 h into the stationary phase that contained intracytoplasmic membranes. Cells harvested 4 and 16 h into the stationary phase contained 58 and 43% more free lipid per 100 mg of cell weight than found in undifferentiated exponential-phase cells. These same cultures were used to compare the quantity of lipid extracted per cell. This analysis revealed 89 and 142% more lipid per cell in 4 and 16 h stationary-phase cells. Further study demonstrated that cells increased in length a,nd decreased in density with time after they entered the stationary phase. We estimated, however, that intracytoplasmic membrane development in G. oxydans is accompanied by a 57 to 62% increase in free lipid that cannot be attributed to a change in cell size. These results suggest that the traditional expression of extracted lipid per milligram of cellular dry weight should not be used for comparative purposes during differentiation in gram-negative bacteria, unless it is first established that both cell size and cell density remain constant throughout differentiation.

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