Quantitative analysis of the expression of the HLA-DRB genes at the transcriptional level by competitive polymerase chain reaction.

Vincent, Rachel; Louis, P; Gongora, Céline; Papa, Ilenia; Clot, J; Eliaou, Jean‐François

doi:10.4049/jimmunol.156.2.603

Cited by 44 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Deduced genomic amino acid sequences are gathered in Supplementary data 4. We used genes and MHC organization of cattle as a reference as they represent an ungulate with a well-assembled genome and welldefined MHC genes (Vincent et al, 1996;Yamamoto et al, 2020;Schwartz et al, 2022).…”

Section: Genome Mining and Choice Of Ampliconsmentioning

confidence: 99%

Functional immune diversity in reindeer reveals a high Arctic population at risk

Lukacs

Nymo²,

Madslien³

et al. 2023

Front. Ecol. Evol.

View full text Add to dashboard Cite

Climate changes the geographic range of both species as well as pathogens, causing a potential increase in the vulnerability of populations or species with limited genetic diversity. With advances in high throughput sequencing (HTS) technologies, we can now define functional expressed genetic diversity of wild species at a larger scale and identify populations at risk. Previous studies have used genomic DNA to define major histocompatibility complex (MHC) class II diversity in reindeer. Varying numbers of expressed genes found in many ungulates strongly argues for using cDNA in MHC typing strategies to ensure that diversity estimates relate to functional genes. We have used available reindeer genomes to identify candidate genes and established an HTS approach to define expressed MHC class I and class II diversity. To capture a broad diversity we included samples from wild reindeer from Southern Norway, semi-domesticated reindeer from Northern Norway and reindeer from the high Artic archipelago Svalbard. Our data show a medium MHC diversity in semi-domesticated and wild Norwegian mainland reindeer, and low MHC diversity reindeer in Svalbard reindeer. The low immune diversity in Svalbard reindeer provides a potential risk if the pathogenic pressure changes in response to altered environmental conditions due to climate change, or increased human-related activity.

show abstract

Section: Genome Mining and Choice Of Ampliconsmentioning

confidence: 99%

Functional immune diversity in reindeer reveals a high Arctic population at risk

Lukacs

Nymo²,

Madslien³

et al. 2023

Front. Ecol. Evol.

View full text Add to dashboard Cite

show abstract

Specific overexpression of rheumatoid arthritis-associated HLA-DR alleles and presentation of low-affinity peptides

Kerlan‐Candon¹,

Louis‐Plence²,

Wiedemann³

et al. 2001

Arthritis & Rheumatism

View full text Add to dashboard Cite

Objective To compare levels of HLA–DR expression in rheumatoid arthritis (RA) patients and healthy controls for whom an ordered expression according to the DR alleles is demonstrated and to test the functional consequences of this expression on peptide presentation. Methods Using monoclonal antibodies that recognize different DRB1 alleles, DR molecules were quantitated at the surface of the peripheral blood B cells of 23 RA patients and 17 healthy subjects. The functional consequences of the level of DR surface expression was tested using a universal model of antigen presentation and mutated peptides with variable affinities for the T cell receptor. Results In healthy subjects, surface HLA–DR molecules were expressed at different levels according to allele (DR53, DR4, and DR11 less than DR1 less than DR7 less than DR15). In RA patients, this hierarchy was not conserved and, furthermore, the density of RA‐associated DR4 and DR1 molecules was enhanced in patients compared with the basal density in healthy individuals. We demonstrated that an increased expression of DR molecules at the surface of antigen‐presenting cells allowed a noteworthy presentation of low‐affinity peptides that under normal conditions are not efficient in generating a T cell response at physiologic surface density of the DR molecules. Conclusion Our results suggest that the specific overexpression of RA‐associated HLA molecules could be responsible for the presentation of low‐affinity autopeptides and therefore the activation of peripheral autoreactive T cells.

show abstract

Differential expression of insulin‐dependent diabetes mellitus‐associated HLA‐DQA1 alleles in vivo

et al. 1997

View full text Add to dashboard Cite

The strong association of HLA-DQ genes with insulin-dependent diabetes mellitus (IDDM) susceptibility is persuasive evidence of their central role in the etiology of this autoimmune disease. Among other possibilities, it has been proposed that an unbalanced expression of IDDM-associated DQA, and/or DQB alleles may lead to alterations in the composition of alpha beta heterodimers and preferential expression of a particular heterodimer on the antigen-presenting cell surface, leading to self-recognition. In this report, we demonstrate the differential expression of DQA1 alleles in vivo, in particular of the two diabetogenic alleles DQA1*0301 and DQA1*0501. Family studies suggest that unequal HLA-DQA1 allele expression in heterozygous individuals is not associated in cis with the HLA-DQA1 gene, but may be affected by trans-acting determinant(s). We also discuss the segregation of this phenotype in IDDM-affected members. Furthermore, we examined historical samples of PBL from an IDDM-affected individual and an HLA-identical unaffected sibling acting in a kidney transplant program as donor and recipient, respectively. This analysis allowed us to establish that unbalanced expression of DQA1*0301 and DQA1*0501 can be induced by microenvironmental conditions. Inducible differential expression of HLA-DQA1 alleles may account for the discordance in the outcome of autoimmune disease in monozygotic twins and HLA-identical siblings.

show abstract

Quantitative analysis of the expression of the HLA-DRB genes at the transcriptional level by competitive polymerase chain reaction.

Cited by 44 publications

References 0 publications

Functional immune diversity in reindeer reveals a high Arctic population at risk

Functional immune diversity in reindeer reveals a high Arctic population at risk

Specific overexpression of rheumatoid arthritis-associated HLA-DR alleles and presentation of low-affinity peptides

Differential expression of insulin‐dependent diabetes mellitus‐associated HLA‐DQA1 alleles in vivo

Contact Info

Product

Resources

About