Quantitative Analysis of the Accumulation of Marine-derived Tocopherol in the Tissue of Mice Fed with Salmon Roe Oil Using HPLC-fluorescence

Abstract

In this study, we measured the quantity of marine-derived tocopherol (MDT), a monounsaturated vitamin E (VE), stored in the body tissue of mice fed with a diet containing a VE-rich fraction extracted from salmon roe. We first prepared the calibration curves for the MDT concentration using an HPLC-fluorescence system. Ranging from 0.016 to 50 μg/mL, the slope was expressed as first-order equations, with R values = 0.99. The mice were fed with an AIN-93 based diet containing MDT in doses of 21.4 mg/kg for 4 weeks, and the storage in the heart, lung, liver, stomach, small intestine, large intestine, kidney, pancreas, spleen, testis, skeletal muscle, visceral white adipose tissue (WAT), subcutaneous WAT and brain was quantified. MDT was widely distributed in tissues throughout the whole body, with higher accumulations observed in the adipose tissue, liver and kidney. These results demonstrate means to estimating the MDT concentration in natural products and in the bodies of animals and contribute to the understanding of the physiological functions of MDT in relation to human health.

Application of Partial Hydrogenation for the Generation of Minor Tocochromanol Homologs and Functional Evaluation of Hydrogenated Tocotrienol-rich Vitamin E Oil in Diabetic Obese Mice

“…In addition, Uchida et al 24 found that the concentrations of α-Toc, α-T3 and γ-T3 in the livers of rats fed with VE-deficient diets decreased in one week, whereas those in WAT did not change for four weeks. Such a metabolic property of WAT has also been observed in our previous study 25 that showed MDT accumulation in the tissues throughout the whole body, with higher accumulations in the WAT. In the present study, we found that in addition to α-T1, other chromanol types of T1 and T2 also accumulated in the WAT, whereas little amount was observed in the liver.…”

Analysis of minor vitamin E homologues in natural products

“…Moreover, minor homologues are likely transferred to human tissues via dietary sources. The presence and accumulation of MDT in human plasma [24] and animal tissue due to dietary MDT [27,28] have been previously reported. The analysis of VE, including minor homologues, is required to examine their distribution in natural products, and their nutritional value in foods.…”

α-Tocomonoenol Is Bioavailable in Mice and May Partly Be Regulated by the Function of the Hepatic α-Tocopherol Transfer Protein

“…Relatively high αT concentrations in adipose tissue were reported before [ 13 , 16 , 25 ] and explained by the comparably low turnover of adipocytes, which slowly accumulated and released αT [ 16 , 26 ]. Adipose tissue was also reported as the main storage for α- and γ-tocotrienols and marine-derived tocopherol [ 14 , 16 , 18 , 25 , 27 ].…”