Quantitative analysis of TEM-8 and CEA tumor markers indicating free tumor cells in the peripheral blood of colorectal cancer patients

Raeisossadati, Reza; Farshchian, Moein; Ganji, Azita; Tavassoli, Alieza; Velayati, Arash; Dadkhah, Ezzat; Chavoshi, S; Bahar, Mostafa Mehrabi; Memar, Bahram; Mashhadi, M T Rajabi; Naseh, Hossein; Forghanifard, Mohammad Mahdi; Moghbeli, Meysam; Moaven, Omeed; Abbaszadegan, Mohammad Reza

doi:10.1007/s00384-011-1230-8

Cited by 24 publications

(12 citation statements)

References 22 publications

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“…Therefore, the TEM8 is recognized as a useful marker for identifying tumor-associated microvessels in CRC [95, 96]. Results of the study of Raeisossadati et al strongly confirmed the usefulness of TEM8 as a biomarker for the detection of CRC patients [97]. Authors found significantly higher circulating mRNA levels of TEM8 in the peripheral blood of CRC patients (22/40) compared to healthy controls (9/40).…”

Section: Tumor Endothelial Markers (Tems)mentioning

confidence: 94%

Biomarkers Discovery for Colorectal Cancer: A Review on Tumor Endothelial Markers as Perspective Candidates

Pietrzyk

2016

Disease Markers

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Colorectal cancer (CRC) is the third most common cancer in the world. The early detection of CRC, during the promotion/progression stages, is an enormous challenge for a successful outcome and remains a fundamental problem in clinical approach. Despite the continuous advancement in diagnostic and therapeutic methods, there is a need for discovery of sensitive and specific, noninvasive biomarkers. Tumor endothelial markers (TEMs) are associated with tumor-specific angiogenesis and are potentially useful to discriminate between tumor and normal endothelium. The most promising TEMs for oncogenic signaling in CRC appeared to be the TEM1, TEM5, TEM7, and TEM8. Overexpression of TEMs especially TEM1, TEM7, and TEM8 in colorectal tumor tissue compared to healthy tissue suggests their role in tumor blood vessels formation. Thus TEMs appear to be perspective candidates for early detection, monitoring, and treatment of CRC patients. This review provides an update on recent data on tumor endothelial markers and their possible use as biomarkers for screening, diagnosis, and therapy of colorectal cancer patients.

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Section: Tumor Endothelial Markers (Tems)mentioning

confidence: 94%

Biomarkers Discovery for Colorectal Cancer: A Review on Tumor Endothelial Markers as Perspective Candidates

Pietrzyk

2016

Disease Markers

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“…Moreover, its specificity is also very high as primers are designed for the particular gene of interest and the whole genomic DNA or RNA can be analyzed in one single reaction. In the detection/evaluation of CTCs from peripheral blood of cancer patients, RT-PCR has been proposed for several genes such as CEA, PTEN, and P27 [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

The Trend of CEACAM3 Blood Expression as Number Index of the CTCs in the Colorectal Cancer Perioperative Course

Taddei

Castiglione

Ringressi

et al. 2015

Mediators of Inflammation

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Pathological stage seems to be the major determinant of postoperative prognosis of solid tumors, but additional prognostic determinants need to be better investigated. The most important tumor marker for colorectal cancer (CRC) is the cell-surface antigen, Carcinoembryonic Antigen (CEA), and its assessment is considered a valuable index of circulating tumor cells (CTCs). In this paper, CEACAM3 evaluation was applied given its great specificity in the CRC. Whole blood from the basilic vein of 38 CRC patients was collected before and at various time intervals after the curative resection. Also, from 20 of them, we have obtained two additional intraoperative samples. CEACAM3 expression was evaluated in all the samples by RT-PCR. CEACAM3 duct values showed a decreasing trend from preoperative through early and later postoperative to 6th-month samples (p < 0.001). The average values of CEACAM3 were related to the cancer size (T stage) (p = 0.034) and WHO stage (p = 0.035). A significant effect of the baseline value of CEACAM3 dCt on the temporal trend has been observed (p < 0.001). In this study, we have demonstrated the CEACAM3 specificity and a perioperative trend of CTCs which is coherent with the clinical/pathological considerations and with previous experimental findings in different cancer types.

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“…The extraction of circulating tumor cells may be a new way to predict the prognosis of the patient [2]. According to many articles written about circulating tumor cells, they were expressed at higher levels in patients with colorectal cancer than in normal control patients [8, 11], and the expression level tended to be higher for higher histological stage of the cancer [8, 12]. Moreover, metastasis and recurrence were shown to occur at an earlier time in a patient who showed expression of circulating tumor cells [3, 8], and their survival rate was reported to be lower as well [3, 13].…”

Section: Discussionmentioning

confidence: 99%

Role of β₁-Integrin in Colorectal Cancer: Case-Control Study

Kim

Chung

et al. 2014

Ann Coloproctol

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PurposeIn the metastatic process, interactions between circulating tumor cells (CTCs) and the extracellular matrix or surrounding cells are required. β1-Integrin may mediate these interactions. The aim of this study was to investigate whether β1-integrin is associated with the detection of CTCs in colorectal cancer.MethodsWe enrolled 30 patients with colorectal cancer (experimental group) and 30 patients with benign diseases (control group). Blood samples were obtained from each group, carcinoembryonic antigen (CEA) mRNA for CTCs marker and β1-integrin mRNA levels were estimated by using reverse transcription-polymerase chain reaction, and the results were compared between the two groups. In the experimental group, preoperative results were compared with postoperative results for each marker. In addition, we analyzed the correlation between the expressions of β1-integrin and CEA.ResultsCEA mRNA was detected more frequently in colorectal cancer patients than in control patients (P = 0.008). CEA mRNA was significantly reduced after surgery in the colorectal cancer patients (P = 0.032). β1-Integrin mRNA was detected more in colorectal cancer patients than in the patients with benign diseases (P < 0.001). In colorectal cancer patients, expression of β1-integrin mRNA was detected more for advanced-stage cancer than for early-stage cancer (P = 0.033) and was significantly decreased after surgery (P < 0.001). In addition, expression of β1-integrin mRNA was significantly associated with that of CEA mRNA in colorectal cancer patients (P = 0.001).ConclusionIn conclusion, β1-integrin is a potential factor for forming a prognosis following surgical resection in colorectal cancer patients. β1-Integrin may be a candidate for use as a marker for early detection of micrometastatic tumor cells and for monitoring the therapeutic response in colorectal cancer patients.

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Quantitative analysis of TEM-8 and CEA tumor markers indicating free tumor cells in the peripheral blood of colorectal cancer patients

Cited by 24 publications

References 22 publications

Biomarkers Discovery for Colorectal Cancer: A Review on Tumor Endothelial Markers as Perspective Candidates

Biomarkers Discovery for Colorectal Cancer: A Review on Tumor Endothelial Markers as Perspective Candidates

The Trend of CEACAM3 Blood Expression as Number Index of the CTCs in the Colorectal Cancer Perioperative Course

Role of β₁-Integrin in Colorectal Cancer: Case-Control Study

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Quantitative analysis of TEM-8 and CEA tumor markers indicating free tumor cells in the peripheral blood of colorectal cancer patients

Cited by 24 publications

References 22 publications

Biomarkers Discovery for Colorectal Cancer: A Review on Tumor Endothelial Markers as Perspective Candidates

Biomarkers Discovery for Colorectal Cancer: A Review on Tumor Endothelial Markers as Perspective Candidates

The Trend of CEACAM3 Blood Expression as Number Index of the CTCs in the Colorectal Cancer Perioperative Course

Role of β1-Integrin in Colorectal Cancer: Case-Control Study

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Role of β₁-Integrin in Colorectal Cancer: Case-Control Study