Quantitative analysis of sulfolipid (sulfoquinovosyl diglyceride) and galactolipids (monogalactosyl and digalactosyl diglycerides) in plant tissues

Roughan, P. G.; Batt, Richard D.

doi:10.1016/0003-2697(68)90261-3

Cited by 295 publications

(82 citation statements)

References 34 publications

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“…Identification of individual glycolipids was carried out by running authentic reference standards simultaneously and also by staining with á-naphthol reagent (Siakotes LIPID PROFILE DURING GROWTH AND SENESCENCE OF Catharanthus roseus and Rouser, 1965) and water spray (used to facilitate observations of translucent areas on the TLC plates) (Gardner, 1968) was employed for identification and analytical purposes. The separated glycolipids were estimated on the basis of sugar released after hydrolysis (Roughan and Batt, 1968).…”

Section: Methodsmentioning

confidence: 99%

Changes in lipid profile during growth and senescence of Catharanthus roseus leaf

Mishra¹,

Tyagi²,

Singh³

et al. 2006

Braz. J. Plant Physiol.

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Various lipid classes and compounds were monitored during the period of leaf emergence to leaf drop of Catharanthus roseus. The expansion to early maturation phase was accompanied by cellular build-up of all major lipid classes, whilst aging and senescence were characterized by their substantial decline, except for the neutral lipids; the leaf monogalactosyl diglyceride/digalactosyl diglyceride ratio decreased from 4.3 (complete maturity) to 2.1 (abscised stage). The early maturation stage was the earliest stage when appreciable amounts of free sterols and fatty acids could be observed. Sterol/phospholipids ratios increased by 68-fold in the abscised leaf as compared to that at full maturity. The unsaturated/saturated fatty acid ratio was far lower in the senescent leaf as compared to that of the fully expanded leaf. The spatial alterations in lipid profiles may be suggestive of concomitant changes in membrane ultrastructure and functions, putatively leading to perturbation of indole alkaloid sequestration capability of the tissues of a species of pharmaceutical significance. Key words: Catharanthus roseus, glycolipids, leaf development, phospholipids, senescence, sterol Alterações no perfil de lipídeos durante o crescimento e a senescência de folhas de Catharanthus roseus: Rastrearamse alterações na composição de vários compostos e classes de lipídeos em folhas de Catharanthus roseus, desde a emergência até a abscisão. Da expansão ao início da maturação da folha, houve incrementos nas concentrações de todas as principais classes de lipídeos, observando-se tendência oposta na senescência, à exceção de lipídeos neutros; a razão monogalactosil diglicerídeo/digalactosil diglicerídeo decresceu de 4.3 (maturidade completa) para 2.1 (abscisão). Quantidades apreciáveis de esteróis e ácidos graxos começaram a ser observadas apenas no início maturação foliar. A razão esteróis/fosofolipídeos aumentou 68 vezes na fase de abscisão em relação à folha em sua completa maturidade. A razão ácidos graxos insaturados/ácidos graxos saturados foi muito menor na folha senescente que na folha jovem completamente expandida. As alterações espaciais no perfil de lipídeos podem ser uma sugestão de alterações concomitantes na função e ultra-estrutura da membrana, acarretando, putativamente, perturbações na capacidade de seqüestro de alcalóides dos tecidos de uma espécie importante, de um ponto de vista farmacêutico.

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Section: Methodsmentioning

confidence: 99%

Changes in lipid profile during growth and senescence of Catharanthus roseus leaf

Mishra¹,

Tyagi²,

Singh³

et al. 2006

Braz. J. Plant Physiol.

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“…["Clacetate was rapidly incorporated into lipids, as evidenced by extraction according to Roughan and Batt (20), but no radioactive ethylene was produced during the subsequent 7 days. Labeling by Vacuum Infiltration.…”

Section: Tmv-infected Tobacco Leavesmentioning

confidence: 99%

Regulation of Ethylene Biosynthesis in Virus-Infected Tobacco Leaves

Laat¹,

Loon²,

Vonk³

1981

Plant Physiol.

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The hypersensitive reaction of Samsun NN tobacco leaves to tobacco mosaic virus (TMV) was accompanied by a large increase in ethylene production, just before necrotic local lesions became visible. Normal and virus-induced ethylene production were both largely inhibited by 0.1 milimolar aminoethoxyvinylglycine indicating that methionine is a main ethylene precursor.The contribution of methionine to ethylene production was estimated by labeling leaves with L-IU-14Clmethionine and comparing the specific activities of methionine within and ethylene produced by the leaf. When taken up through the petiole, methionine was largely retained in the veins, leading to production of ethylene with a far higher specific activity in the veins than in the interveinal tissue. After TMV infection, ethylene production increased only in the interveinal tissue, resulting in a decrease in specific activity of the ethylene produced. In the interveinal tissue, the specific radioactivity of the ethylene was lower than expected if methionine were the only precursor. After labeling by vacuum infiltration, the specific activities of the ethylene produced by water-and TMV-inoculated leaves were both identical and in accordance with the specific radioactivity of methionine. Inasmuch as the content of 1-aminocyclopropane-1-carboxylic acid was increased severalfold two days after TMV infection, methionine can be considered to be the only ethylene precursor in healthy and in TMV-infected tobacco leaves.The increase in ethylene production after TMV-infection was not accompanied by an increased concentration of free methionine within the leaf. Compartmentation of methionine does not appear to be a regulating factor since labeled methionine supplied to the leaf by vacuum infiltration is equilibrated very rapidly with any methionine pool within the leaf ceUls.

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“…Glycerolipids for analysis were separated from the remainder by a combination of DEAE-cellulose chromatography and t.l.c. (Roughan & Batt, 1968. Distributions of 140 between the polar and fatty acid moieties of isolated lipids were determined after hydrolysis on the thin-layer adsorbent in 1m-H2SO4 (Roughan &;Batt, 1968).…”

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confidence: 99%

“…(Roughan & Batt, 1968. Distributions of 140 between the polar and fatty acid moieties of isolated lipids were determined after hydrolysis on the thin-layer adsorbent in 1m-H2SO4 (Roughan &;Batt, 1968). The 14C in the hydrolysate and that remaining on the washed centrifuged silica gel were determined separately.…”

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confidence: 99%

Turnover of the glycerolipids of pumpkin leaves. The importance of phosphatidylcholine

Roughan¹

1970

Biochemical Journal

118

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Between 1 and 5% of the 14C recovered from pumpkin leaves within 15-60min after pulse-labelling with 14C02 was in the lipids. The specific radioactivity of the phospholipids was higher than that of the glycolipids. Phosphatidylcholine had five times the specific radioactivity of monogalactosyl diglyceride, and the specific radioactivity of neither galactolipid changed significantly between 1 and 48 h after labelling. It therefore seemed unlikely that the galactose moieties of the galactolipids were involved in the transport of assimilated compounds across the chloroplast membrane. Within 60min of the application of [1-14C]acetate to the surfaces of mature, intact pumpkin leaves 70% of the recovered 14C was in the lipid fraction. Of the separated glycerolipids, phosphatidylcholine had by far the highest specific radioactivity at the shorter time-intervals, and the glycolipids again had the lowest specific radioactivities. Phosphatidylcholine was the only lipid to show a significant turnover of radiocarbon as judged by the decrease in specific radioactivity with time. From a comparison of the changes with time of the labelling of fatty acid fractions from phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and monogalactosyl diglyceride, it is suggested that the primary site of linolenic acid biosynthesis in leaf cells is within the phosphatidylcholine molecule.

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Quantitative analysis of sulfolipid (sulfoquinovosyl diglyceride) and galactolipids (monogalactosyl and digalactosyl diglycerides) in plant tissues

Cited by 295 publications

References 34 publications

Changes in lipid profile during growth and senescence of Catharanthus roseus leaf

Changes in lipid profile during growth and senescence of Catharanthus roseus leaf

Regulation of Ethylene Biosynthesis in Virus-Infected Tobacco Leaves

Turnover of the glycerolipids of pumpkin leaves. The importance of phosphatidylcholine

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