Quantitative analysis of sperm mRNA in the pig: relationship with early embryo development and capacitation

Hwang, Jae Yeon; Mulligan, Brendan P.; Kim, Hyung-Min; Yang, Byoung-Chul; Lee, Chang Kyu

doi:10.1071/rd12160

Cited by 36 publications

(28 citation statements)

References 39 publications

(62 reference statements)

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“…The importance of heat shock proteins in developing embryos is well known (Neuer et al 2000;Zhang et al 2011;Driver and Khatib 2013), though the paternal RNA contribution to protein synthesis must be more precisely defined. A further study with the employment of an higher number of boars and with the possibility to utilize a CASA system to evaluate semen parameters, will be useful to confirm our preliminary data and verify the correlation between the transcripts and semen quality and to evaluate if these transcripts are active and related to embryonic development after fertilization, as hypothesized by some authors (Ostermeier et al 2004;Kempisty et al 2008;Hwang et al 2013). …”

Section: Discussionmentioning

confidence: 50%

“…In swine species, over the past 10 years, transcriptome analysis has identified many mRNAs involved in different biological processes (Yang et al 2009). More recently, the presence of various transcripts has been investigated in order to clarify their involvement in early embryo development (Hwang et al 2013).…”

Section: Discussionmentioning

confidence: 99%

“…In swine, transcriptome analysis of ejaculated spermatozoa has been performed, identifying many mRNAs involved in biological processes, molecular functions and cellular components (Yang et al 2009). More recently, the presence of various transcripts has been investigated in order to describe mRNA involvement in capacitation and early embryo development in pigs (Hwang et al 2013). It is well known that semen quality can be influenced by occasional physical stress, infection, and variation in temperature (Althouse et al 2000;Althouse and Lu 2005;Kunavongkrit et al 2005;Knox et al 2008;Althouse 2008) and the definition of new markers for evaluation of semen could offer knowledge about the fertility potential of a semen sample.…”

Section: Introductionmentioning

confidence: 99%

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Relative abundance of heat shock proteins and clusterin transcripts in spermatozoa collected from boar routinely utilised in an artificial insemination centre: preliminary results

et al. 2017

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It is widely accepted that mature sperm contains RNA. The first hypothesis was that sperm RNAs have no functions of their own but are simply residues of spermatogenesis reflecting the events that occurred during their formation in the testes. More recently new discoveries have essentially expanded these views, showing that sperm mRNAs constitute a population of stable full-length transcripts, many of which are selectively retained during spermatogenesis and delivered to oocytes contributing to early embryo development. It is well known that semen quality can be influenced by occasional physical stress, infection, and variation in temperature and the definition of new markers for evaluation of semen could offer knowledge about the fertility potential of a semen sample. The aim of the present study was to evaluate the presence and the relative quantity of transcripts and protein of heat shock protein 70 (HSP70), 90 (HSP90) and clusterin (CLU) in Percoll-selected spermatozoa collected from seven adult boars of proven fertility routinely employed for artificial insemination. Our results showed the presence of HSP70, HSP90 and CLU transcripts with different level of expression: high for HSPs and low for CLU transcripts. The transcript level of both HSPs are similar among selected spermatozoa derived from high quality sperm with the exception of one boar that showed a reduced content of HSP70 and HSP90 mRNA together with a lower semen quality. At protein level, both HSPs were detected with similar amount among all seven boars whilst no band was evidenced for CLU protein.

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Section: Discussionmentioning

confidence: 50%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Relative abundance of heat shock proteins and clusterin transcripts in spermatozoa collected from boar routinely utilised in an artificial insemination centre: preliminary results

et al. 2017

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“…[26][27][28][29] For example, levels of PRM1 mRNA in the spermatozoa of men, bulls, and boars are positively correlated with fertility. [29][30][31] In other studies, the spermatozoa of highly fertile bulls were found to contain increased concentrations of testis-specific serine kinase 6 (TSSK6) mRNA compared with spermatozoa from bulls with lower fertility. 17,30 In addition to mRNAs, spermatozoa carry long non-coding RNAs (lncRNAs).…”

Section: Introductionmentioning

confidence: 94%

Identification and quantification of coding and long non‐coding RNAs in stallion spermatozoa separated by density

Ing

Konganti²,

Ghaffari

et al. 2020

Andrology

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Background It is not unusual for stallions to have fertility problems. For many, artificial insemination with more dense spermatozoa (isolated by density gradient centrifugation) results in greater pregnancy rates compared with the rates when using unfractionated spermatozoa. RNAs in spermatozoa delivered to the oocyte at conception are required for embryo development. Novel molecular assays of spermatozoa that reflect function are needed to predict the fertility of stallions. Objectives To describe and compare the RNA populations in more dense and less dense spermatozoa from stallions. Materials and methods Spermatozoa from five stallions were separated into more dense and less dense populations by density gradient centrifugation. Complementary DNA libraries were made from each of the ten total RNA samples after ribosomal RNA removal. Next‐generation sequencing characterized the RNA populations in more and less dense spermatozoa. Quantitative reverse transcription‐PCR was used to confirm differential expression of selected RNAs. Results Stallion spermatozoa contain 11 215 RNAs, with the most prevalent RNA being a 1492 base long non‐coding RNA. The levels of 159 RNAs were greater in more dense spermatozoa, while levels of seven other RNAs were greater in less dense spermatozoa. Quantitative reverse transcription‐PCR confirmed the threefold greater levels of solute carrier family 26 member 8 (SLC26A8) mRNA in less dense spermatozoa, and sixfold and threefold greater expression levels of the SCP2 sterol binding domain containing 1 (SCP2D1) and spermatogenesis‐associated protein 31D1 (SPATA31D1) mRNAs in more dense spermatozoa, respectively. Discussion and conclusion We identified 11 215 RNAs in stallion spermatozoa and 166 with differential expression between more dense and less dense fractions. Many prevalent RNAs were also found in bull, boar, and human spermatozoa. Many differentially expressed RNAs are known to be testis‐ or spermatozoa‐specific. Our results may lead to identification of an RNA population in spermatozoa that is optimal for establishing successful pregnancies.

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“…IVF embryos were obtained following the method described in our previous study (Hwang et al 2013b). Briefly, the prepubertal gilt ovaries donated by the Sooam Biotech Research Institute (Seoul, Korea) were used in this experiment.…”

Section: Ivf Embryomentioning

confidence: 99%

Identification and differential expression patterns of porcine OCT4 variants

Hwang

Lee

et al. 2015

Reproduction

Self Cite

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OCT4 encoded by POU5F1 has a crucial role of maintaining pluripotency in embryonic stem cells during early embryonic development and several OCT4 variants have been identified in mouse and human studies. The objective of this study was to identify different variants of OCT4 and analyze their expression patterns in preimplantation porcine embryos and various tissues. In this study, we showed that POU5F1 transcribes its three variants, namely OCT4A, OCT4B, and OCT4B1. The OCT4B transcript consists of exons identical to the major form of the OCT4 variant, OCT4A, with a differential N-terminal domain-coding exon. The structure of OCT4B1 mRNA was the same as that of OCT4B mRNA, but harbored a cryptic exon. Based on these findings, the transcription levels were investigated and found that OCT4B and OCT4B1 made up w20% among the variants in the embryonic stage and this indicates that OCT4A mRNA is dominantly expressed during preimplantation embryo development. In addition, OCT4B mRNA was detected in all tissues examined, while OCT4A and OCT4B1 were detected only in testis but not in other tissues examined. OCT4B1 showed inversely correlated expression with SOX2 and NANOG expression. OCT4A protein was specifically localized to the nuclei, whereas OCT4B was mainly localized to the cytoplasm of the porcine embryos at the blastocyst stage. The findings of this study reveal that the porcine OCT4 gene can potentially encode three variants (OCT4A, OCT4B, and OCT4B1), and they are differentially expressed and would have roles dissimilar between each other in preimplantation embryos and various adult tissues.

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Quantitative analysis of sperm mRNA in the pig: relationship with early embryo development and capacitation

Cited by 36 publications

References 39 publications

Relative abundance of heat shock proteins and clusterin transcripts in spermatozoa collected from boar routinely utilised in an artificial insemination centre: preliminary results

Relative abundance of heat shock proteins and clusterin transcripts in spermatozoa collected from boar routinely utilised in an artificial insemination centre: preliminary results

Identification and quantification of coding and long non‐coding RNAs in stallion spermatozoa separated by density

Identification and differential expression patterns of porcine OCT4 variants

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