Quantitative analysis of serum lipids with thin-layer chromatography

Gloster, J; Fletchek, R.F.

doi:10.1016/0009-8981(66)90298-1

Cited by 80 publications

(19 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…11 Total cholesterol mass (Boehringer Mannheim CHOD-PAP 12 ) and total radioactivity were determined on the eluates. Lipids were separated by thin-layer chromatography, 13 and the proportion of radioactivity in cholesterol and cholesterol ester was determined from videorecorded images of autoradiograms by using KS400 (Carl Zeiss) image analysis software.…”

Section: Aortic Tissuementioning

confidence: 99%

Direct Assessment of Lipoprotein Outflow From In Vivo–Labeled Arterial Tissue as Determined in an In Vitro Perfusion System

Björnheden¹,

Bondjers²,

Wiklund³

1998

ATVB

View full text Add to dashboard Cite

Abstract-The rate of cholesterol deposition during the atherosclerotic process is determined by the balance between the inflow and outflow of plasma lipoproteins in the arterial wall. Whereas the rate of inflow may be measured directly, the rate of outflow has most often been calculated indirectly from lipoprotein uptake by using the 2-compartment model. One objection against such calculations is that lipoprotein binding is not being considered. In the present study 2 different protocols were used to obtain a direct measure of the outflow of lipoproteins from atherosclerotic rabbit aortas. Thus, 3 rabbits with experimental atherosclerosis were given 125 I-LDL intravenously and 3 were given [ 14 C]cholesterol perorally. Twenty-four hours later the aortas were removed and the outflow of label was monitored during in vitro perfusion. Despite the different protocols, our results were consistent and indicated that fractional loss relative to whole tissue was Ϸ0.01 pool/h, which is 1 order of magnitude lower than current estimates based on the 2-compartment model (0.04 to 0.4 pool/h). Furthermore, whereas as much as 2 ⁄3 to 3 ⁄4 of the tracer that had entered the arterial wall was effectively trapped, the remainder equilibrated at a faster rate (0.06 pool/h). In conclusion, it seems that tissue binding constitutes a prominent and possibly underrated mechanism of lipoprotein deposition, at least in the atherosclerotic rabbit aorta. Furthermore, this means that current estimates of lipoprotein exchange parameters based on the 2-compartment model (eg, fractional loss) may rest on invalid assumptions and should be regarded with caution.

show abstract

Section: Aortic Tissuementioning

confidence: 99%

Direct Assessment of Lipoprotein Outflow From In Vivo–Labeled Arterial Tissue as Determined in an In Vitro Perfusion System

Björnheden¹,

Bondjers²,

Wiklund³

1998

ATVB

View full text Add to dashboard Cite

show abstract

“…Thin layer chromatography was used to separate neutral lipids on one portion of the eluate 13 and phospholipids on the other.…”

Section: Radioactivity In Lipidsmentioning

confidence: 99%

A system for long-term perfusion of rabbit aorta in vitro.

Björnheden¹,

Bylock²,

Hansson³

et al. 1983

Arteriosclerosis

View full text Add to dashboard Cite

In an in vitro system for the perfusion of arterial tissue, the volume of the incubation chamber should be small, and the composition of the medium should be easily modified. The tissue should consist of media and intima only, and the interaction with the medium should occur via the intimal side. Consecutive sampling of the medium and the tissue should be possible. This paper describes a system with these characteristics. Scanning and transmission electron microscopy of the perfused tissue indicated that the endothelium was intact during the first day and that it still covered more than 95% of the surface after 3 days. On the 2nd day, the nonthrombogenic properties of the endothelium were maintained. The medial smooth muscle cells of the inner twothirds of the preparation were viable during the perfusion, while the cells in the outer one-third were dead from the start. Still, the metabolic activity of the tissue was stable, at least during the 2nd day as assessed by the study of DNA, protein, and lipid synthesis, as well as by oxygen consumption. We conclude that the perfusion system presented here might be useful in the study of the interaction between cellular and humoral components of the blood and the arterial wall.

show abstract

“…The cell pellets were extracted with chloroform-methan01 and thin layer chromatography was performed in accordance with the metllocl of Gloster and Fletcher [6]. Each lipid fraction was scraped from the plate and washed several times with chloroform.…”

Section: T H I N Layer Chromatographymentioning

confidence: 99%