Quantitative analysis of ruminal bacterial populations involved in lipid metabolism in dairy cows fed different vegetable oils

Vargas‐Bello‐Pérez, Einar; Cancino‐Padilla, Nathaly; Romero, Jaime; Garnsworthy, P. C.

doi:10.1017/s1751731116000756

Cited by 35 publications

(26 citation statements)

References 41 publications

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“…However, our inference cannot be specific for Butyrivibrio fibrisolvens . Similar mechanisms may occur with other bacteria species such as Butyrivibrio proteoclasticus and Prevotella bryantii (Potu, AbuGhazaleh, Hastings, Jones, & Ibrahim, ; Vargas‐Bello‐Pérez, Cancino‐Padilla, Romero, & Garnsworthy, ). This may be a reasonable explanation for the decrease in IVpdNDFD only for MS and SC, which have greater NDS fraction.…”

Section: Discussionmentioning

confidence: 63%

Effect of soya bean oil supplementation and forage type on methane production and fibre digestibility using the in vitro gas production system

Rodrigues

Ramin

Huhtanen

et al. 2017

Grass and Forage Science

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We aimed to evaluate the effect of soya bean oil (SBO) supplementation with different forages on in vitro gas production kinetics, methane (CH4) emissions and potentially digestible neutral detergent fibre (pdNDF) digestibility (IVpdNDFD). Samples of whole‐crop maize silage (MS; Zea mays), sugarcane (SC; Saccharum sp.), perennial ryegrass (RG; Lolium perenne), guinea grass (GG; Panicum maximum) and palisadegrass (PG; Brachiaria brizantha) were incubated with three concentrations of SBO (0, 30 and 60 g/kg of dry matter). The interaction between forage species and SBO inclusion affected molar proportion of acetate, acetate‐to‐propionate ratio, asymptotic gas and IVpdNDFD. Acetate‐to‐propionate ratio numerically decreased from 3.56 to 3.44 and 3.77 to 3.56 for MS and SC respectively. Soya bean oil inclusion at 60 g/kg DM decreased 21.2% and 12.9% of IVpdNDFD for MS and SC respectively. Soya bean oil increase did not affect IVpdNDFD for RG, GG and PG. Soya bean oil inclusion decreased quadratically the asymptotic gas (294–265 ml/g OM) for MS and increased linearly (275–283 ml/g OM) for GG. Lower and greater CH4 production was observed for RG and SC respectively. Methane production decreased quadratically by SBO increase. Soya bean oil inclusion linearly decreased CH4 concentration in total gas at 48 hr of incubation from 133 to 128 ml/L. In conclusion, MS and SC are more sensitive to adverse effects of SBO supplementation from 30 to 60 g/kg DM on rumen fermentation when compared to RG, GG and PG.

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Section: Discussionmentioning

confidence: 63%

Effect of soya bean oil supplementation and forage type on methane production and fibre digestibility using the in vitro gas production system

Rodrigues

Ramin

Huhtanen

et al. 2017

Grass and Forage Science

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“…This result was broadly reflected in the results measuring the total microbial protein synthesis (g/d). An inhibitory effect of oils on rumen bacteria has been reported in a number of studies in vivo (Vargas-Bello-Pérez et al, 2016) and in vitro pure cultures (Maia et al, 2010) although in these cases, a higher dose may have contributed to a greater effect.…”

Section: Discussionmentioning

confidence: 92%

Effect of Sunflower and Marine Oils on Ruminal Microbiota, In vitro Fermentation and Digesta Fatty Acid Profile

et al. 2017

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This study using the rumen simulation technique (RUSITEC) investigated the changes in the ruminal microbiota and anaerobic fermentation in response to the addition of different lipid supplements to a ruminant diet. A basal diet with no oil added was the control, and the treatment diets were supplemented with sunflower oil (2%) only, or sunflower oil (2%) in combination with fish oil (1%) or algae oil (1%). Four fermentation units were used per treatment. RUSITEC fermenters were inoculated with rumen digesta. Substrate degradation, fermentation end-products (volatile fatty acids, lactate, gas, methane, and ammonia), and microbial protein synthesis were determined. Fatty acid profiles and microbial community composition were evaluated in digesta samples. Numbers of representative bacterial species and microbial groups were determined using qPCR. Microbial composition and diversity were based on T-RFLP spectra. The addition of oils had no effect on substrate degradation or microbial protein synthesis. Differences among diets in neutral detergent fiber degradation were not significant (P = 0.132), but the contrast comparing oil–supplemented diets with the control was significant (P = 0.039). Methane production was reduced (P < 0.05) with all oil supplements. Propionate production was increased when diets containing oil were fermented. Compared with the control, the addition of algae oil decreased the percentage C18:3 c9c12c15 in rumen digesta, and that of C18:2 c9t11 was increased when the control diet was supplemented with any oil. Marine oils decreased the hydrogenation of C18 unsaturated fatty acids. Microbial diversity was not affected by oil supplementation. Cluster analysis showed that diets with additional fish or algae oils formed a group separated from the sunflower oil diet. Supplementation with marine oils decreased the numbers of Butyrivibrio producers of stearic acid, and affected the numbers of protozoa, methanogens, Selenomonas ruminantium and Streptococcus bovis, but not total bacteria. In conclusion, there is a potential to manipulate the rumen fermentation and microbiota with the addition of sunflower, fish or algae oils to ruminant diets at appropriate concentrations. Specifically, supplementation of ruminant mixed rations with marine oils will reduce methane production, the acetate to propionate ratio and the fatty acid hydrogenation in the rumen.

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“…Other remarkable distinctions occurred in the Euryarchaeota, Spirochaetes, and Fibrobacteres phyla, which were hardly detected in the other three groups, as well as the genus Prevotella , which showed obvious increase in the WT.HC group (Figure c and d). Prevotella , which mostly colonizes in obese individuals, contains members involved in carbohydrate and amino acid fermenting, as well as acetate and hydrogen producers (Vargas‐Bello‐perez, Cancino‐Padilla, Romero, & Garnsworthy, ). Euryarchaeota is the main archaeal species that colonized the intestine (Horz & Conrads, ).…”

Section: Resultsmentioning

confidence: 99%

“…A similar variation tendency was observed for the relative abundance of several phylum or species, including the genera Prevotella, Ruminococcus , and Oscillospira , and the phyla Euryarchaeota and Spirochaetes. Prevotella is involved in the fermentation of carbohydrates and amino acids and is also a producer of acetate and hydrogen (Vargas‐Bello‐perez et al., ). These H 2 ‐producing components were often detected in combination with a relatively high abundance of Archaea , which are H 2 ‐oxidizing methanogenic organisms, for example, Euryarchaeota.…”

Section: Discussionmentioning

confidence: 99%

Relative variations of gut microbiota in disordered cholesterol metabolism caused by high‐cholesterol diet and host genetics

Tao

Shao

et al. 2017

MicrobiologyOpen

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Recent studies performed provide mechanistic insight into effects of the microbiota on cholesterol metabolism, but less focus was given to how cholesterol impacts the gut microbiota. In this study, ApoE−/− Sprague Dawley (SD) rats and their wild‐type counterparts (n = 12) were, respectively, allocated for two dietary condition groups (normal chow and high‐cholesterol diet). Total 16S rDNA of fecal samples were extracted and sequenced by high‐throughput sequencing to determine differences in microbiome composition. Data were collected and performed diversity analysis and phylogenetic analysis. The influence of cholesterol on gut microbiota was discussed by using cholesterol dietary treatment as exogenous cholesterol disorder factor and genetic modification as endogenous metabolic disorder factor. Relative microbial variations were compared to illustrate the causality and correlation of cholesterol and gut microbiota. It turned out comparing to genetically modified rats, exogenous cholesterol intake may play more effective role in changing gut microbiota profile, although the serum cholesterol level of genetically modified rats was even higher. Relative abundance of some representative species showed that the discrepancies due to dietary variation were more obvious, whereas some low abundance species changed because of genetic disorders. Our results partially demonstrated that gut microbiota are relatively more sensitive to dietary variation. Nevertheless, considering the important effect of bacteria in cholesterol metabolism, the influence to gut flora by “genetically caused cholesterol disorder” cannot be overlooked. Manipulation of gut microbiota might be an effective target for preventing cholesterol‐related metabolic disorders.

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Quantitative analysis of ruminal bacterial populations involved in lipid metabolism in dairy cows fed different vegetable oils

Cited by 35 publications

References 41 publications

Effect of soya bean oil supplementation and forage type on methane production and fibre digestibility using the in vitro gas production system

Effect of soya bean oil supplementation and forage type on methane production and fibre digestibility using the in vitro gas production system

Effect of Sunflower and Marine Oils on Ruminal Microbiota, In vitro Fermentation and Digesta Fatty Acid Profile

Relative variations of gut microbiota in disordered cholesterol metabolism caused by high‐cholesterol diet and host genetics

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