Quantitative Analysis of Retinal Ganglion Cell Survival with Rbpms Immunolabeling in Animal Models of Optic Neuropathies

Abstract

Rbpms can reliably be used as an RGC marker for quantitative evaluation in rat models of RGC degeneration, regardless of the nature and the location of the primary site of the injury and the extent of neurodegeneration.

Assessment of retinal ganglion cell damage in glaucomatous optic neuropathy: Axon transport, injury and soma loss

“…These studies show an almost complete RBPMSþ/FGþ colocalization before and after IOP elevation (Kwong et al, 2010(Kwong et al, , 2011, suggesting that RBPMS is a robust RGC label for experimental GON research. Furthermore, Rodriguez et al (2014) compared RBPMS immunoreactivity with that of various amacrine cell labels (GABA, glycine and syntaxin 1) and demonstrated that RBPMS was not found in appreciable levels in amacrine cells.…”

“…To determine the specificity of RBPMS to RGCs, Kwong et al (2010Kwong et al ( , 2011 compared RBPMS immunoreactivity with retrograde FG administration to the ON stump. These studies show an almost complete RBPMSþ/FGþ colocalization before and after IOP elevation (Kwong et al, 2010(Kwong et al, , 2011, suggesting that RBPMS is a robust RGC label for experimental GON research.…”

A retinal code for motion along the gravitational and body axes

“…1a–d). All were ganglion cells (immunopositive for RNA-binding protein with multiple splicing 20 ; n = 706; Extended Data Fig. 2a–f).…”

Multipronged Approach to Study Glaucoma-Associated Phenotypes

“…First, a major challenge lies on the scarce number of RGCs that can be isolated from murine retinae (148,204,205). Second, current signature markers for the identification of RGCs are intracellular markers (206)(207)(208), impeding the isolation of viable, metabolically active cells downstream for in vitro studies. Third, current protocols are lengthy and have not been standardized for the isolation of primary murine RGCs from dissociated retinae.…”

“…Classically, IHC analyses of cross sections of ganglion cell layer flat mounts are used for the detection of surface and intracellular markers associated with RGC (207,208,238,239). Despite its extensive use there are a series of limitations such as fading and photobleaching of fluorescence, limited sensitivity and the presence of false-negative or false-positive results.…”