Quantitative Analysis of Representative Proteome Components and Clustering of Helicobacter pylori Clinical Strains

Abstract

These results indicated that quantitative analysis of proteome components is a feasible method for examining disease-associated proteins and clustering clinical strains of H. pylori.

Differential Proteomics of Helicobacter pylori Associated with Autoimmune Atrophic Gastritis

“…An additional property in interaction with the extracellular matrix of infected host cells was also proposed by Backert et al (71). High levels of EF-Tu expression in HP isolates from GC patients have been reported (32,62). In our work, the lowest content of EF-Tu in the HP proteome associated with AAG was accompanied by a low content of another protein involved in protein biosynthesis: the ribosome-recycling or -releasing factor, responsible for the release of ribosomes from mRNA at the termination of translation (72).…”

“…Its occurrence in HP proteomes is also documented in some bacterial strains associated with CG, GC and DU (24,62). In the pH range of 3.5-5.0, HP is known to maintain the proton motive force (PMF) across its periplasmic membrane, ensuring a continued supply of energy through ATP synthesis (63).…”

“…This enzyme converts one molecule of inorganic pyrophosphate into two phosphate ions by a highly exergonic reaction. This protein was identified in HP proteome isolated from chronic gastritis, DU and GC (13,24,62), with a higher expression in GC with respect to the proteome of HP from patients with gastritis (73).…”

Association between Chronic Atrophic Gastritis and Serum Antibodies to 15 Helicobacter pylori Proteins Measured by Multiplex Serology

“…Eight of these markers have been shown to be associated with gastric diseases (gastric cancer and peptic ulcer) in clinical settings: Cad, CagM, HP0305, HpaA, HyuA, GroEL, NapA, and UreA (14,16,45). However, among these, only catalase and GroEL were investigated in an epidemiologic study (18), and none of these antibodies have been analyzed with respect to the development of CAG.…”

Biomarkers and diagnostic tools for detection of Helicobacter pylori

“…Previous researches have focused on the urease enzymes, CagA, VacA, FlaA, HspB, FlaB, and outer membrane proteins in order to quickly produce a diagnostic kit for recognition of H. pylori infection (Cremonini et al 2004;Khalilpour et al 2013;Schumann et al 2006;Zheng et al 2002). Although some bacterial factors such as the heat shock protein, H. pylori adhesin (HpaA), and flagella have also been identified as pathogenic determinants, understanding the function of H. pylori cellular components in the pathogenesis of gastric disorders requires further investigation (Park et al 2006). In addition, a few unidentified antigenic bands such as 18, 39.5, 33, and 34 kDa have been reported to be of good diagnostic value (Andersen and Espersen 1992;Galmiche et al 2000;Keenan et al 2000).…”