Quantitative analysis of ras gene mutation in pancreatic secretions for diagnosis of pancreatic carcinoma: Comparison with three different assays

Tada, Minoru; Tateishi, Kazunari; Yamagata, Motoyuki; Isayama, Hiroyuki; Komatsu, Yutaka; Kawabe, Touichi; Yoshida, Hikaru; Shiratori, Yasushi; Omata, Masao

doi:10.1016/s0016-5085(98)82036-2

Cited by 4 publications

(2 citation statements)

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“…DNA was extracted from the pancreatic juice by the standard phenol/chloroform method 14. We recently obtained similar results to the semiquantitative assay of mutant K- ras gene using our modified allele specific PCR9 and two other methods: ( a ) PCR/PHFA (Wakunaga Pharmaceutical Corporation, Hiroshima, Japan and SRL Corporation, Tokyo, Japan); ( b ) enriched PCR/ELMA (BML Corporation, Tokyo, Japan) 15. The two assays are available as handy kits.…”

Section: Methodssupporting

confidence: 59%

“…All materials were measured by both assays. Because similar amounts of mutant gene were obtained to those previously reported,15 the amount obtained by enriched PCR/ELMA was used for the results. The scores (3+), (2+), (1+), (+−), and (−) represented approximately more than 20%, 2–20%, 0.2–2%, less than 0.2%, and 0%—that is, not detected—of the mutant respectively according to the manufacturer.…”

Section: Methodsmentioning

confidence: 99%

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High proportion of mutant K-ras gene in pancreatic juice of patients with pancreatic cystic lesions

Tateishi

Tada

Yamagata

et al. 1999

Gut

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Background/Aims-It was recently reported that the quantitative analysis of mutant K-ras gene in pancreatic juice could be useful for the diagnosis of pancreatic cancer. This methodology was applied to patients with pancreatic cystic lesions. Methods-DNA was extracted from pancreatic juice collected at the time of endoscopy with injection of secretin. The ratio of the K-ras mutant allele to the wild-type allele was measured by two methods to detect and semiquantify mutant K-ras gene: polymerase chain reaction/preferential homoduplex formation assay and enriched polymerase chain reaction/enzyme linked mini-sequence assay. Results-A high frequency of K-ras mutation was detected (more than 2% of all K-ras genes) in six of 14 patients (43%) with pancreatic cysts. This frequency was similar to those detected in patients with pancreatic adenocarcinoma and in intraductal papillary neoplasms of the pancreas. In contrast, the frequency of mutation was low (less than 2%) in patients without either pancreatic cysts or pancreatic neoplasms. Conclusions-K-ras gene mutation may be derived from duct cells in the pancreas with a high potential for tumorigenesis. Therefore careful follow up of patients with a pancreatic cyst is recommended if they are found to have a high level of the mutant gene in the pancreatic juice. (Gut 1999;45:737-740)

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Section: Methodssupporting

confidence: 59%

Section: Methodsmentioning

confidence: 99%