Quantitative analysis of ractopamine in beef using automated online sample preparation with liquid chromatography-tandem mass spectrometry

Lafontaine, Catherine; Yu, Honglin; Espourteille, Francois A.; Yang, Sen

doi:10.1039/c2ay25497b

Cited by 9 publications

(5 citation statements)

References 7 publications

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“…This combination makes the mobile phase flow no more laminar decreasing the affinity of large macromolecules with the stationary phase, making this technique proper to remove proteins and others macromolecules from the sample . This was applied to the analysis of royal jelly , beef , honey , and chicken meat.…”

Section: Instrumentation For MDCmentioning

confidence: 99%

An overview of multidimensional liquid phase separations in food analysis

et al. 2016

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Food safety is a priority public health concern that demands analytical methods capable to detect low concentration level of contaminants (e.g. pesticides and antibiotics) in different food matrices. Due to the high complexity of these matrices, a sample preparation step is in most cases mandatory to achieve satisfactory results being usually tedious, lengthy, and prone to the introduction of errors. For this reason, many research groups have focused efforts on the development of online systems capable to do the cleanup, concentration, and separation steps at once through multidimensional separation techniques (MDS). Among several possible setups, the most popular are the multidimensional chromatographic techniques (MDC) that consist in combining more than one mobile and/or stationary phase to provide a satisfactory separation. In the present review, we selected a variety of multidimensional separation systems used for food contaminant analysis in order to discuss the instrumentation aspects, the concept of orthogonality, column approaches used in these systems, and new materials that can be used in these columns. Selected classes of contaminants present in food matrices are introduced and discussed as example of the potential applications of multidimensional liquid phase separation techniques in food safety.

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Section: Instrumentation For MDCmentioning

confidence: 99%

An overview of multidimensional liquid phase separations in food analysis

et al. 2016

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“…Turbulent flow chromatography (TFC) was developed as an analytical tool in the mid 1990s, combining ‘size exclusion’ and traditional stationary phase column chemistries to separate macromolecules, such as proteins, from smaller molecules and analytes of interest in biological fluids , environmental and food samples. Turbulent flow has been now widely used as an automated on‐line sample preparation technique coupled to LC allowing direct and fast injection of complex samples in the analytical system .…”

Section: Turbulent Flow Chromatographymentioning

confidence: 99%

“…Although the on‐line turbulent flow chromatography technique has been employed to the development of rapid, high sensitivity, and robust methods for determination of pharmaceuticals in food matrices , so far few papers were reported in the literature describing the use of this technique in the food analysis area. Nevertheless, this approach enables quick and high sensitivity analysis for food matrices . Furthermore, the TFC technique allows the determination of analytes from different classes utilizing the same procedure .…”

Section: Turbulent Flow Chromatographymentioning

confidence: 99%

Recent approaches for on‐line analysis of residues and contaminants in food matrices: A review†

Barreiro

Luiz

Maciel

et al. 2015

J of Separation Science

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This review highlights recent developments for on-line determination of residues and contaminants in complex matrices such as food samples. This involves the on-line coupling of a sample preparation technique (as the first "dimension") with a chromatographic system (second "dimension"), usually followed by mass spectrometry. Although frequently treated as quite distinct techniques, the role of all devices utilized as the first dimension in this approach aims to decrease the sample complexity while eliminating as much as possible the matrix contaminants to facilitate the qualitative and quantitative determination of the compounds of interest. This review will focus on the following techniques as the first dimension: (i) on-line solid-phase extraction; (ii) in-tube solid-phase microextraction; (iii) matrix solid-phase dispersion; and (iv) turbulent flow chromatography. The second dimension is usually performed using a chromatographic column to isolate the analyte(s) of interest for further mass spectrometry determination. A description of the basis of this on-line approach and its distinct set up possibilities is presented, which is followed by a critical review of the literature covering this subject in the last ten years (focusing on the last five years) with emphasis on the analysis of residue and contaminants in food samples.

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“…[3] The World Anti-Doping Agency (WADA) had explicitly classified ractopamine as a prohibited substance in the β 2 agonist category in the "2019 Prohibited List International Standard". [4] Various methods have been developed for the detection of ractopamine, including high-performance liquid chromatography, liquid chromatography-mass spectrometry, [5,6] enzyme-linked immunoassay [7][8][9] and fluorescence immunoassay. [10][11][12] However, the analysis of ractopamine and other veterinary drug residues poses challenges due to complex sample matrices, low analyte concentrations, and numerous interfering substances.…”

Section: Introductionmentioning

confidence: 99%

Efficient Synthesis of Ractopamine‐D₃ via Epoxide‐Opening Strategy

Wang,

Wu,

Chen

et al. 2023

Asian J Org Chem

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The incorporation of deuterium isotopes into illicit additives like ractopamine holds significant promise for enabling swift and accurate detection. However, current synthetic methodologies for deuterium labeling often involve intricate and time‐consuming processes, necessitating laborious de/protection steps and specialized equipment. Herein, after establishing efficient epoxide‐opening reactions using diverse primary amines, a practical, efficient and straightforward synthetic route for producing deuterium‐labeled ractopamine‐D3 has been successfully established, which was confirmed by 1H‐NMR and ESI‐MS with a deuterium isotope abundance exceeding 98 atom% D. Remarkably, this newly devised convergent synthetic protocol, encompassing just three steps, delivered up to 53% overall yield of ractopamine‐D3, underscoring its scalability and potential pharmaceutical viability.

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Quantitative analysis of ractopamine in beef using automated online sample preparation with liquid chromatography-tandem mass spectrometry

Cited by 9 publications

References 7 publications

An overview of multidimensional liquid phase separations in food analysis

An overview of multidimensional liquid phase separations in food analysis

Recent approaches for on‐line analysis of residues and contaminants in food matrices: A review†

Efficient Synthesis of Ractopamine‐D₃ via Epoxide‐Opening Strategy

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Quantitative analysis of ractopamine in beef using automated online sample preparation with liquid chromatography-tandem mass spectrometry

Cited by 9 publications

References 7 publications

An overview of multidimensional liquid phase separations in food analysis

An overview of multidimensional liquid phase separations in food analysis

Recent approaches for on‐line analysis of residues and contaminants in food matrices: A review†

Efficient Synthesis of Ractopamine‐D3 via Epoxide‐Opening Strategy

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Product

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Efficient Synthesis of Ractopamine‐D₃ via Epoxide‐Opening Strategy