Quantitative analysis of race-specific resistance to Colletotrichum lindemuthianum in common bean

Oblessuc, Paula Rodrigues; Baroni, Renata Moro; Pereira, Guilherme da Silva; Chiorato, Alisson Fernando; Carbonell, Sérgio Augusto Morais; Briñez, Boris; Silva, Luciano da Costa e; Garcia, Antônio Augusto Franco; Camargo, L. E. A.; Kelly, James D.; Benchimol-Reis, Luciana Lasry

doi:10.1007/s11032-014-0118-z

Cited by 35 publications

(44 citation statements)

References 78 publications

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“…Group 1 of the Structure analysis ( Table 2 , Figure 3 ) was composed predominantly of Andean large-seeded genotypes directed for export driven by market demand, such as Feijão Suíço, Chileno/Branco, Branco Argentino, Amendoim, Bagajo, Jalo, and Jalo-110. Another feature observed in this group was the reddish color of the tegument that characterizes the Red Kidney and Vermelhinho cultivars and most of the lines derived from the CAL-143 x IAC-UNA (C x U) and IAC-UNA x CAL-143 (U x C) crosses used for the UC map ( Campos et al , 2011 ; Oblessuc et al , 2012 , 2014 ).…”

Section: Resultsmentioning

confidence: 92%

Developing a common bean core collection suitable for association mapping studies

Perseguini

Silva

Rosa³

et al. 2015

Genet. Mol. Biol.

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Because of the continuous introduction of germplasm from abroad, some collections have a high number of accessions, making it difficult to explore the genetic variability present in a germplasm bank for conservation and breeding purposes. Therefore, the aim of this study was to quantify and analyze the structure of genetic variability among 500 common bean accessions to construct a core collection. A total of 58 SSRs were used for this purpose. The polymorphism information content (PIC) in the 180 common bean accessions selected to compose the core collection ranged from 0.17 to 0.86, and the discriminatory power (DP) ranged from 0.21 to 0.90. The 500 accessions were clustered into 15 distinct groups and the 180 accessions into four distinct groups in the Structure analysis. According to analysis of molecular variance, the most divergent accessions comprised 97.2% of the observed genetic variability present within the base collection, confirming the efficiency of the selection criterion. The 180 selected accessions will be used for association mapping in future studies and could be potentially used by breeders to direct new crosses and generate elite cultivars that meet current and future global market needs.

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Section: Resultsmentioning

confidence: 92%

Developing a common bean core collection suitable for association mapping studies

Perseguini

Silva

Rosa³

et al. 2015

Genet. Mol. Biol.

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“…The race 04 of C . lindemuthianum was used in this study because it previously detected ANT QTLs in greenhouse conditions [ 32 ], and the race 0–39 of P . griseola was also previously associated to ALS QTLs [ 30 , 31 ].…”

Section: Methodsmentioning

confidence: 99%

“…Several groups have performed AM studies for common bean [ 27 , 28 , 29 ], but none have focused on ANT and ALS resistance. Based on several linkage analysis studies, we have reported previously important resistance candidate genes in common bean [ 30 , 31 , 32 , 33 ]. However, to complement our initial findings and further understand the ANT and ALS plant disease interactions in common bean, the aims of this study were to (i) estimate the linkage disequilibrium (LD) in common bean; and (ii) identify QRLs associated with resistance to ANT and ALS using SSR and SNP data.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Association Studies of Anthracnose and Angular Leaf Spot Resistance in Common Bean (Phaseolus vulgaris L.)

et al. 2016

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The common bean (Phaseolus vulgaris L.) is the world’s most important legume for human consumption. Anthracnose (ANT; Colletotrichum lindemuthianum) and angular leaf spot (ALS; Pseudocercospora griseola) are complex diseases that cause major yield losses in common bean. Depending on the cultivar and environmental conditions, anthracnose and angular leaf spot infections can reduce crop yield drastically. This study aimed to estimate linkage disequilibrium levels and identify quantitative resistance loci (QRL) controlling resistance to both ANT and ALS diseases of 180 accessions of common bean using genome-wide association analysis. A randomized complete block design with four replicates was performed for the ANT and ALS experiments, with four plants per genotype in each replicate. Association mapping analyses were performed for ANT and ALS using a mixed linear model approach implemented in TASSEL. A total of 17 and 11 significant statistically associations involving SSRs were detected for ANT and ALS resistance loci, respectively. Using SNPs, 21 and 17 significant statistically associations were obtained for ANT and angular ALS, respectively, providing more associations with this marker. The SSR-IAC167 and PvM95 markers, both located on chromosome Pv03, and the SNP scaffold00021_89379, were associated with both diseases. The other markers were distributed across the entire common bean genome, with chromosomes Pv03 and Pv08 showing the greatest number of loci associated with ANT resistance. The chromosome Pv04 was the most saturated one, with six markers associated with ALS resistance. The telomeric region of this chromosome showed four markers located between approximately 2.5 Mb and 4.4 Mb. Our results demonstrate the great potential of genome-wide association studies to identify QRLs related to ANT and ALS in common bean. The results indicate a quantitative and complex inheritance pattern for both diseases in common bean. Our findings will contribute to more effective screening of elite germplasm to find resistance alleles for marker-assisted selection in breeding programs.

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“…Anthracnose resistance genes Co-4 and its alleles and Co-2 are located on Pv08 and Pv11, respectively ( Young et al, 1998 ; Melotto and Kelly, 2001 ; Alzate-Marin et al, 2007 ; Rodriguez-Suarez et al, 2007 ). In addition to major resistance genes, studies showed that anthracnose resistance is quantitatively (QTLs) inherited like ANT02.1 UC and ANT07.1 UC ( Oblessuc et al, 2014 ). Recently, a major QTL for anthracnose resistance against race 1545 was mapped to Pv05 with candidate gene Phvul.005G117900 that encodes a NBS-LRR protein ( Gonzalez et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

North-Western Himalayan Common Beans: Population Structure and Mapping of Quantitative Anthracnose Resistance Through Genome Wide Association Study

et al. 2020

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Common bean ( Phaseolus vulgaris L.) is an important legume crop of north-western (NW) Himalayan region and the major disease that causes catastrophic loss to the crop is anthracnose, which is caused by Colletotrichum lindemuthianum . The pathogen is highly diverse and most of the commercial cultivars are susceptible to different races prevalent in the region. The lack of information on the genomic regions associated with anthracnose resistance in NW Himalayan common bean population prompted us to dissect Quantitative Resistance Loci (QRLs) against major anthracnose races. In this study, 188 common bean landraces collected from NW region were screened against five important anthracnose races and 113 bean genotypes showed resistance to one or multiple races. Genotyping by sequencing (GBS) was performed on a panel of 192 bean lines (4 controls plus 188 Indian beans) and 22,589 SNPs were obtained that are evenly distributed. Population structure analysis of 192 bean genotypes categorized 188 Indian beans into two major clusters representing Andean and Mesoamerican gene pools with obvious admixtures. Many QRLs associated with anthracnose resistance to Indian C. lindemuthianum virulences (race 3, 87, and 503) are located at Pv04 within the gene models that encode typical resistance gene signatures. The QRLs associated with race 73 are located on Pv08 and overlaps with Co-4 anthracnose resistance gene. A SNP located at distal end of Pv11 in a gene model Phvul.011G202300 which encodes a LRR with a typical NB-ARC domain showed association with race 73 resistance. Common bean genomic regions located at Pv03, Pv09, and Pv11 showed association with resistance to anthracnose race 2047. The present study showed presence of many novel bean genomic regions associated with anthracnose resistance. The presence of Co-4 and Co-2 genes in our material is encouraging for breeding durable anthracnose resistant cultivars for the region.

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Quantitative analysis of race-specific resistance to Colletotrichum lindemuthianum in common bean

Cited by 35 publications

References 78 publications

Developing a common bean core collection suitable for association mapping studies

Developing a common bean core collection suitable for association mapping studies

Genome-Wide Association Studies of Anthracnose and Angular Leaf Spot Resistance in Common Bean (Phaseolus vulgaris L.)

North-Western Himalayan Common Beans: Population Structure and Mapping of Quantitative Anthracnose Resistance Through Genome Wide Association Study

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