Quantitative analysis of polysaccharides from Plantago major leaves using the Dreywood method

Оленников, Д. Н.; Танхаева, Л. М.; Samuelsen, Anne Berit

doi:10.1007/s10600-006-0096-4

Cited by 30 publications

(22 citation statements)

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“…Physicochemical characteristics of melanoidin were determined according to the following methods: total sugar content -anthrone-sulphuric method with D-glucose as standard (Olennikov et al, 2006) and protein content using the Bradford method with bovine serum albumin as a standard (Bradford, 1976). The carboxyl group content and total phenol group content were determined using potentiometric titration according to the method of Koroleva et al (2007).…”

Section: Methodsmentioning

confidence: 99%

Physicochemical characteristics and antioxidant activity of melanoidin pigment from the fermented leaves of Orthosiphon stamineus

Оленников¹,

Танхаева²

2012

Rev. bras. farmacogn.

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Abstract:The melanoidin pigment (OS-M) was isolated from fermented leaves of Orthosiphon stamineus Benth. (Lamiaceae), with a 0.37% yield (from dry plant weight), and characterised. OS-M is a phenolic polymer with a molecular weight of 4.4 kDa. Determination of basic physicochemical parameters using elemental analysis, functional group analysis, UV-Vis-and FTIR-spectroscopy of OS-M indicated that the isolated polymer was similar to typical melanoidins. Experimental data show that aromatic fragments dominate the OS-M structure, which also contains a small amount of aliphatic fragments.

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Section: Methodsmentioning

confidence: 99%

Physicochemical characteristics and antioxidant activity of melanoidin pigment from the fermented leaves of Orthosiphon stamineus

Оленников¹,

Танхаева²

2012

Rev. bras. farmacogn.

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“…The carbohydrate content was determined by the anthrone method [4]; phenols, by the Folin method [5]; N, by the Bradford method using Coumassi G250 (ZAO Sileks) [6]; ash, gravimetrically after ashing; acetyl content, by reaction with hydroxylamine [7]; and optical rotation, on a Coers polarimeter, l = 10 cm at 20°C. Spectrophotometric studies were carried out on a Cecil CE 2011 spectrophotometer in 10-mm quartz cuvettes.…”

Section: Methodsmentioning

confidence: 99%

Lamiaceae carbohydrates. IV. Water-soluble polysaccharides from Scutellaria baicalensis

2008

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We continued research on the carbohydrate components of plants from the familly Lamiaceae [1] by isolating and characterizing preliminarily water-soluble polysaccharides (WSPS) from the aerial part of Scutellaria baicalensis Georgi.Lipophilic substances were removed from S. baicalensis raw material, which was then treated with ethanol (80%) to afford a fraction (10.36-11.39% of absolute dry raw material mass) that contained the free carbohydrates glucose, galactose, and saccharose.WSPS were isolated from the remaining solid by extraction with hot water, concentration, and precipitation with ethanol to afford fraction WSPS, which was a colored substance with protein, phenol, and ash components ( Table 1). The ash elements of WSPS included 26 elements, the main components of which were Ca, Mg, Na, P, and Si (Table 2). In general, the elemental composition of the WSPS was similar to that of the aerial part of S. baicalensis [2].Removal of minerals and proteins from WSPS produced the complex WSPS′ in 18.33% yield (of WSPS mass) that had positive optical rotation and gave a weak color with iodine solution. Total hydrolysis produced galactose, arabinose, and glucose in a 3.2:1.3:1.0 ratio (Table 1). Fractional precipitation of WSPS′ by ethanol separated five components WSPS′-1, WSPS′-2, WSPS′-3, WSPS′-4, and WSPS′-5. Table 3 lists the principal physicochemical properties of them.The dominant components WSPS′-1, WSPS′-2, and WSPS′-3 had positive optical rotation [α] D 20 , did not react with iodine, and consisted of galactose, arabinose, and glucose in 4.6:1.5:1, 3.8:2.4:1, and 28.7:8.7:1 ratios, respectively. The weak reaction with iodine of the total complex was due to the presence of WSPS′-4 and WSPS′-5, which probably were starch-type glucans (only glucose was detected in the hydrolysate). Gel chromatography showed that WSPS′-1, WSPS′-2, and WSPS′-3 were homogeneous with molecular weights (MW) 23, 27, and 35 kDa, respectively; WSPS′-4 and WSPS′-5 were heterogeneous and contained two substances each with MW 11 and 22 kDa in 14:7 and 3:5 ratios, respectively. IR spectra of WSPS′-1, WSPS′-2, and WSPS′-3 differed from those of WSPS′-4 and WSPS′-5 (Table 3). In the range 700-1000 cm −1 , WSPS′-1, WSPS′-2, and WSPS′-3 exhibited absorption bands for α-bonds of three types that were due to ring vibrations analogous to dioxane vibrations (type 1, 914-917 cm −1 ), deformation (equatorial) vibrations (type 2a, 834-835 cm −1 ); and pyranose-ring vibrations (type 3, 762-765 cm −1 ). Bands in the ranges 860-864 and 981-985 cm −1 were typical of arabino-3,6-galactans [3]. IR spectra of WSPS′-1, WSPS′-2, and WSPS′-3 also contained maxima for absorption of esters (1239-1264 and 1719-1738 cm −1 ) that disappeared after treatment of the solutions with base. This was consistent with the presence of acetyls in the polymer structures, the content of which was 2.87-3.15% (Table 3). 557TABLE 1. Physicochemical Properties of WSPS and WSPS′ Component Yield, % Content, % [α] D

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“…The contents of carbohydrate components were determined by the anthrone method [6]; of phenols, by the literature method [7] calculated as gallic acid; of protein, by the ninhydrin method after hydrolysis and calculated as alanine [8]; ash content, by gravimetry after ashing; acetyl content, by reaction with hydroxylamine [9]; optical rotation, on a Coers polarimeter with l = 10 cm at 20°C; and pH, using a Checker™1 pH-meter (Hanna Instruments). Spectrophotometric studies were performed on a Cecil CE 2011 spectrophotometer in 10-mm quartz cuvettes.…”

Section: Methodsmentioning

confidence: 99%

Lamiaceae carbohydrates. 1. Pectinic substances and hemicelluloses from Mentha x piperita

Оленников

Танхаева

2007

Chem Nat Compd

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Pectinic substances from the aerial part of Mentha x piperita were isolated and characterized and found to be a mixture of β-(1→4)-glucogalactan (MPG) and two α-(1→4)-rhamnopolygalacturonans . It was shown that the pectin and its components exhibited membrane-stabilizing, antiatherogenic, and antioxidant activity. Hemicelluloses from M. piperita were a lignocarbohydrate complex. Mentha x piperita L. (Lamiaceae) is an official plant material that is widely used in medical practice. The main properties of pectinic substances (PS) from the variety Krasnodar 116 [1] and polysaccharides from tissue culture of domestic [2] and foreign [3,4] varities of this species have been previously described.Herein we report results from the isolation and study of the composition of PS and hemicellulose (HC) from the aerial part of M. piperita Krasnodar 2 variety.We found 5.23-6.13% PS containing 97.02 ± 2.01% carbohydrates and <0.02% phenolic compounds with 0.85 ± 0.16% ash content, 0.52 ± 0.02% nitrogen, [α] D 20 +189° (c 0.1, water), and pH 5.5-5.8 (c 0.1, water) in the aerial part of M. x piperita.The GalUA content was 54.5% with the ratio of neutral carbohydrates in the total complex PS being Glc:Gal:Ara:Rha: Xyl = 28:25:5:4:1 (Table 1). The quantitative characteristics were free carboxylic group content (K C ), 5.35 ± 0.11%; esterified carboxylic groups (K E ), 4.39 ± 0.14%; esterification degree (λ), 45-46%.PS were separated using calcium pectinate in order to determine the component composition. This produced acidic and neutral components, MPP and MPG, respectively. Saponification by base of MPP gave demethoxylated pectinic acid MPP′, which was confirmed by analyzing the functional groups: K C = 9.48 ± 0.28%; K E = 0.34 ± 0.08%; λ = 3-4%.Gel chromatography showed that MPP′ consisted of two components MPP′-1 and MPP′-2 in the ratio 1:2.3 with MW 6.0·10 4 and 3.7·10 4 Da, respectively. The monosaccharide compositions of these were identical and differed only by the lack of Xyl in MPP′-2. Partial hydrolysis of MPP′-1 and MPP′-2 formed the same chromatographically homogeneous component MPP′′, which contained 97.3% GalUA and 2.7% Rha. The molecular weight of MPP′′ according to gel chromatography was 3.0·10 4 Da; the degree of polymerization, 185. Enzymatic hydrolysis of MPP′′ by pectinase produced GalUA. The positive specific rotation of MPP′′ indicated that the glycoside bonds had the α-configuration. This was confirmed by the insignificant decrease of the specific rotation of the polyacetate (MPP′′-Ac) compared with starting MPP′′. The content of acetyls corresponded to substitution of two hydroxyls in anhydrogalacturonyl units. The IR spectrum of MPP′′ contained the following absorption bands (cm -1 ): 1725 (carboxylic C=O); 1610 and 1400 (ionized carboxylic); 1028, 1050, 1110 (pyran ring), and 832 (α-glycoside bond). The bands at 740 and 922 cm -1 shifted compared with pure GalUA (775 and 904 cm -1 ). This is characteristic of polysaccharides with a 1→4 bond.Methoxylated polygalacturonan was prepared and subjected to perioda...

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Quantitative analysis of polysaccharides from Plantago major leaves using the Dreywood method

Cited by 30 publications

References 2 publications

Physicochemical characteristics and antioxidant activity of melanoidin pigment from the fermented leaves of Orthosiphon stamineus

Physicochemical characteristics and antioxidant activity of melanoidin pigment from the fermented leaves of Orthosiphon stamineus

Lamiaceae carbohydrates. IV. Water-soluble polysaccharides from Scutellaria baicalensis

Lamiaceae carbohydrates. 1. Pectinic substances and hemicelluloses from Mentha x piperita

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