Quantitative Analysis of Polyphenols and Biological Activity of Sage Macerates

Abstract

The total phenols concentration in two sage macerates has been estimated by Folin-Cioc�lteau method, identified and quantified using HPLC-DAD method in order to assess the biological activity. The results for total phenols values of Folin Ciocalteau method indicate that Salvia officinalis L macerate S2, presents a higher amount of phenolic compounds than macerate S1. By HPLC-DAD method, six individual phenolic compounds were identified in sage macerates among which where cinnamic acid was found in highest concentration (652.478 mg/100g d.w. in S2 and 473.381 mg/100g d.w. in S1). The antioxidant activity of sage macerates was evaluated using DPPH Radical Scavenging test. Sage macerates exhibited high antioxidant activity, between 439.5 mg GAE /mL and 400 mg GAE /mL. Antibacterial activity of sage macerates was evaluated against 20 Gram positive and Gram negative bacterial strains isolated from clinical specimens. Both macerates showed significant but variable antibacterial activity with inhibition zones ranging from 4.97 mm (S2) to 7.28.mm (S1). The effect was stronger on Gram positive (Enterococcus, Staphyococcus) than Gram negative bacteria (Escherichia sp, Proteus sp, Klebsiella sp). Eleven metals concentrations were determined by AAS method in sage leaves; it has been found that Cd, Ni and Pb concentrations are below the detection limits.

Nutritional, Antioxidant, Antimicrobial, and Toxicological Profile of Two Innovative Types of Vegan, Sugar-Free Chocolate

“…In the alfalfa extract we obtained similar results to those reported by Karimi et al [ 31 ]. Interestingly, our investigation was not able to identify the presence of GA in sage, as compared to a previous study [ 56 ].…”

“…The highest ferulic acid (FE) content was identified in the green tea extract whereas the lowest values were detected in sage, as previously reported by other studies [ 56 ]; however, the literature reports much higher concentrations of FE in stevia (0.86–5.5 mg/g) compared to our experimental data [ 57 ].…”

“…Similar values to those reported by literature data were observed for the coumaric acid (CU) in blackcurrant [53] and sage [56].…”

Chemical Composition, Antioxidant Activity, and Antibacterial Activity of Black Poplar Buds’ Hydroalcoholic Macerates from Dobrogea Area

“…Molecules 2023, 28, 4920 2 of 13 The Populus species has great potential for therapeutic applications: in the treatment of dermatitis, rheumatism, upper respiratory tract infections, and skin aging [5][6][7][8][9]. Poplar bud extract has also been reported for use in the treatment of oropharyngeal cavity, urinary tract, digestive/excretory tract, and bacterial infections [3,7,10].…”

“…Poplar bud extract has also been reported for use in the treatment of oropharyngeal cavity, urinary tract, digestive/excretory tract, and bacterial infections [ 3 , 7 , 10 ]. Exploring the antimicrobial properties of natural products has become a significant research effort [ 11 , 12 , 13 , 14 , 15 ]. A significant antimicrobial effect of the biological components present in the buds, leaves, and branches of black poplar has been reported [ 14 ].…”