Quantitative analysis of phospholipids by HPLC with a light scattering evaporating detector – application to raw materials for cosmetic use

Becart, I.; Chevalier, C.; Biesse, J.P.

doi:10.1002/jhrc.1240130210

Cited by 143 publications

(94 citation statements)

References 16 publications

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“…Reported detection limits for the ELSD indicate that sample sizes consistent with natural levels can be easily detected (Becart et al, 1990;Letter, 1992;Nordback et aI., 1998). However, since lipid P represents only a small fraction of total sediment P, no further modifications of the HPLC method were attempted for this study.…”

Section: Detection Limit and Use For Natural Samplesmentioning

confidence: 99%

Organic phosphorus in marine sediments : chemical structure, diagenetic alteration, and mechanisms of preservation

Laarkamp¹

2000

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Phosphorus, an essential nutrient, is removed from the oceans only through burial with marine sediments. Organic phosphorus (Po,g) constitutes an important fraction (ca. 25%) oftotal-P in marine sediments. However, given the inherent lability of primary Po,g biochemicals, it is a puzzle that any Pm, is preserved in marine sediments. The goal of this thesis was to address this apparent paradox by linking bulk and molecular-level Po" information.A newly-developed sequential extraction method, which isolates sedimentary Po" reservoirs based on solubility, was used in concert with 3lp nuclear magnetic resonance spectroscopy (31p_NMR) to quantify Po,g functional group concentrations. The coupled extraction/ 31 P-NMR method was applied to three sediment cores from the Santa Barbara Basin, and the first-ever high-resolution depth profiles of molecular-level Po,g distribution during diagenesis were generated.These depth profiles were used to consider regulation of Pm, distribution by biomass abundance, chemical structure, and physical protection mechanisms. Biomass cannot account for more than a few percent of sedimentary Po,g. No evidence for direct structural control on remineralization of Po,g was found. Instead, sorptive protection appears to be an important mechanism for Po,g preservation, and structure may act as a secondary control due to preferential sorption of specific Pm, compound classes.

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Section: Detection Limit and Use For Natural Samplesmentioning

confidence: 99%

Organic phosphorus in marine sediments : chemical structure, diagenetic alteration, and mechanisms of preservation

Laarkamp¹

2000

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“…[Guo et al, 2005;Kisel et al, 2001;Vannieuwenhuyzen, 1981]. In contrast with the polar lipid fraction of plants, dairy products contain a substantial part of sphingolipids, which can be used as raw material for the production of ceramides, applicable in the cosmetic industry [Becart et al, 1990]. Due to its major role in maintaining the water-retaining properties of the epidermis, ceramide is of great commercial potential in cosmetic and pharmaceutical industries, such as in hair and skin care products [Zhang et al, 2006].…”

Section: Functionalities and Applications Of Mfgmmentioning

confidence: 99%

Composition, Properties and Nutritional Aspects of Milk Fat Globule Membrane – A Review

El-Loly¹

2011

Pol. J. Food Nutr. Sci.

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processes and defense mechanisms in the newborn [Cavaletto et al., 2006].The stabilizing membrane acts as reactive sorts on the interface between the barriers of milk serum. As such, it can be globule and rate-controlling for a host of physical and of enzymes chemical interactions, e.g., binding mace elements; controlled release of the polar materials products of lipolysis; transfer of milk serum; maintenance of emulsion into stability by prevention of globule fusion; availability of fatty acids and cholesterol for micellar absorption in the small intestine; and destabilization by creaming, clumping, churning, freeze-thaw, and heating, resulting in loosely bound substances into milk transfer serum. Worthy of notice is that the interactions are dynamic.Moreover, it is assumed that the MFGM proteins also possess specific nutritional properties. As such, due to their origin, composition and structure, MFGM polar lipids and proteins could be used as an emulsifier or stabilizer, combining technological and nutritional functionality [Mulder & Walstra, 1974;Anderson & Cawston, 1975;Patton & Keenan, 1975;Patton & Jensen, 1975, 1976Keenan et al., 1983;Mather & Keenan, 1983; McPherson & Kitchen, 1983; Walstra & Jenness, 1984;Keenan & Dylewski, 1985 Bucbheim, 1986;Mather, 1987; Keenan et al., 1988;Keenan & Dylewski, 1995;Keenan & Patton, 1995;Mather & Keenan, 1998;Danthine et al., 2000; Keenan, 2001; Keenan & Mather, 2002; Ollivier--Bousquet, 2002;Lopez et al., 2006Lopez et al., , 2007Sanchez-Juanes et al., 2009;Zamora et al., 2009]. Composition, Properties and Nutritional Aspects of Milk Fat Globule Membrane -a Review Mohamed Mansour El-Loly Dairy Department, National Research Centre, Dokki, Cairo, EgyptKey words: milk fat globule membrane, mammary gland, isolation, structure, stability, nutritional, nutraceuticalIn the last few years, knowledge on the composition and properties of the milk fat globule membrane (MFGM) increased significantly. It is now recognized that the MFGM is highly complex in structure and composed of different protein and lipid components with specific technological and nutritional properties. As such, MFGM materials have been isolated and characterized as valuable ingredients for incorporation into new food products. However, MFGM are also sensitive to modification during isolation and processing, and care should be taken to standardize the composition and characteristics of the membrane to maintain its unique properties during application in food products.The MFGM is subject to changes in composition and structure from the moment the fat globule leaves the mammary secretory cell. Upon milk harvesting and further milk handling, further changes to the MFGM take place. Depending on the type and degree of treatment, this may involve different physico-chemical interactions between various membrane components, the loss of membrane components and/or adsorption of components from the milk plasma. However, the effects appear to be variable and dependent on physiological (animal) factors, and much remains ...

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“…Many authors (Breton et al, 1989;Bunger and Pison, 1995;Olsson et al, 1996;Seppanen-Laakso et al, 2001;Silversand and Haux, 1997;Stith et al, 2000) have made modifications on the original method by Becart and Biesse (1990) for the separation and quantitation of phospholipids with HPLC-ESLD in a variety of biological samples. Table 2 provides a summary of detection methods that have been used in conjunction with Stith et al (2000) Xenopus laevis oocytes ELSD Bunger and Pison (1995) Lung lavage ELSD Breton et al (1989) PS, PC, PE, PG, PI ELSD Gunnarsson et al (1997) PI, PA, PG, PE, PC, PS, SM ELSD Grizard et al (2000) Human semen ELSD Patton et al (1990) Rat liver UV Lesnefsky et al (2000) Rabbit heart subsarcolemmal UV mitochondria Guan et al (2001) Human, mouse and rat brain Radioactive flow tissue; mouse heart; rat liver detector Thevenon et al (1998) Rat thymocytes Fluorescence Seppanen-Laakso et al (2001) Human plasma ELSD Silversand and Haux (1997) Salmon liver and eggs ELSD Olsson et al (1996) Human brain ELSD Homan and Anderson (1998) Rat liver, heart, kidney and brain ELSD Rodriguez-Bernaldo Sea urchin UV de Quiros et al (2002) Bernhard et al (1995) Rat and pig gastric mucosa /mucus UV/Fluorescence Gross et al (2000) Porcine While TLC, GC and HPLC can all be used to separate, and to some extent quantitate, phospholipids, all of these methods are time-intensive and require multiple experimental steps.…”

Section: Stationary Phasesmentioning

confidence: 99%

A review of chromatographic methods for the assessment of phospholipids in biological samples

Peterson

Cummings

2005

Biomedical Chromatography

235

159

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Phospholipids are important constituents of all living cell membranes. Lipidomics is a rapidly growing field that provides insight as to how specific phospholipids play roles in normal physiological and disease states. There are many analytical methods available for the qualitative and quantitative determination of phospholipids. This review provides a summary of the methods that were historically used such as thin layer chromatography, gas chromatography and high-performance liquid chromatography. In addition, an introduction to applications of interfacing these traditional chromatographic techniques with mass spectrometry is provided.

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Quantitative analysis of phospholipids by HPLC with a light scattering evaporating detector – application to raw materials for cosmetic use

Cited by 143 publications

References 16 publications

Organic phosphorus in marine sediments : chemical structure, diagenetic alteration, and mechanisms of preservation

Organic phosphorus in marine sediments : chemical structure, diagenetic alteration, and mechanisms of preservation

Composition, Properties and Nutritional Aspects of Milk Fat Globule Membrane – A Review

A review of chromatographic methods for the assessment of phospholipids in biological samples

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