Quantitative analysis of peptide–MHC class II interaction

Fleckenstein, Burkhard; Wiesmüller, Karl‐Heinz

doi:10.1006/smim.1999.0198

Cited by 24 publications

(9 citation statements)

References 77 publications

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“…4B, the results of our modifications for one such algorithm, ISC-PLS (110), applied to two MHC alleles are shown. These results are consistent with those of past experimental studies showing that peptide length affects binding to a number of MHC alleles (128,129).…”

Section: Modelssupporting

confidence: 93%

Toward a multiscale model of antigen presentation in immunity

Kirschner

Chang

Riggs

et al. 2007

Immunological Reviews

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A functioning immune system and the process of antigen presentation in particular encompass events that occur at multiple length and time scales. Despite a wealth of information in the biological literature regarding each of these scales, no single representation synthesizing this information into a model of the overall immune response as it depends on antigen presentation is available. In this article, we outline an approach for integrating information over relevant biological and temporal scales to generate such a representation for major histocompatibility complex class II-mediated antigen presentation. In addition, we begin to address how such models can be used to answer questions about mechanisms of infection and new strategies for treatment and vaccines.

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Section: Modelssupporting

confidence: 93%

Toward a multiscale model of antigen presentation in immunity

Kirschner

Chang

Riggs

et al. 2007

Immunological Reviews

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“…Combinatorial peptide libraries have been used successfully to give insight into peptide binding requirements of MHC class II molecules [18,19]. The determination of the peptide binding motif of HLA-DQ4 is of special interest as its association with one subtype of JIA has been described [6,7].…”

Section: Discussion Activity Pattern and Peptide Binding Motif Of Hlamentioning

confidence: 99%

Determination of the peptide binding motif and high-affinity ligands for HLA-DQ4 using synthetic peptide libraries

et al. 2004

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“…In this study, we employed a quantitative implemented peptide binding motif analysis (24)(25)(26)(27) to identify promiscuous human leukocyte antigen (HLA) class II epitopes encoded in the ESAT-6 protein. We then designed 2 multiepitopic ESAT-6 peptides that were predicted to elicit cellular immune responses in peripheral blood mononuclear cells from individuals with active TB.…”

Section: Introductionmentioning

confidence: 99%

Identification of Early Secretory Antigen Target-6 Epitopes for the Immunodiagnosis of Active Tuberculosis

Vincenti

Carrara

Mori³

et al. 2003

Mol Med

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The early secretory antigenic target (ESAT)-6 purified protein and peptides from Mycobacterium tuberculosis were evaluated as antigens for the immunodiagnosis of tuberculosis (TB). Because the control of TB requires improved diagnostic procedures, efforts have increased to identify Mycobacterium tuberculosis-specific epitopes for the immunodiagnosis of active TB and to discriminate between active and latent states of infection. Two multiepitopic peptides from ESAT-6 protein were selected by computational analysis. Patients with active TB (7 HIV + and 20 HIV-) and control patients (17 HIV + and 28 HIV-) were enrolled. Enzyme-linked immunospot assay analysis for interferon-γ expression by peripheral blood mononuclear cells was quantified after stimulation with selected ESAT-6 peptides, purified protein derivative, or the intact ESAT-6 protein. During active TB, 20 of 27 patients responded in vitro to ESAT-6 peptides and 23 of 27 patients to purified protein derivative. None of the controls without active TB, including individuals with latent TB infection, recognized ESAT-6 peptides. By contrast, latently infected individuals did respond in vitro to both intact ESAT-6 protein and purified protein derivative. Thus, high T-cell response frequencies to ESAT-6 peptides are present only during active TB and can be used to discriminate between active and latent forms of infection.

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Quantitative analysis of peptide–MHC class II interaction

Cited by 24 publications

References 77 publications

Toward a multiscale model of antigen presentation in immunity

Toward a multiscale model of antigen presentation in immunity

Determination of the peptide binding motif and high-affinity ligands for HLA-DQ4 using synthetic peptide libraries

Identification of Early Secretory Antigen Target-6 Epitopes for the Immunodiagnosis of Active Tuberculosis

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