Quantitative analysis of oxytetracycline and related substances by high-performance liquid chromatography

Khan, Naeem Hasan; Roets, Eugène; Hoogmartens, Jos; Vanderhaeghe, Hubert

doi:10.1016/s0021-9673(01)81765-9

Cited by 50 publications

(10 citation statements)

References 14 publications

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“…LC methods have been described for the separation of OTC and its impurities [8 10]. With the isocratic LC method described by Khan et al [10,11], utilizing poly (styrene-divinylbenzene) copolymer (PSDVB) as stationary phase, ADOTC was partially separated from the main component for the first time, but the peaks are broad and therefore, the sensitivity is rather poor. This method is official in the Ph.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of an improved liquid chromatographic method for the analysis of oxytetracycline

et al. 2002

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SummaryAn improved LC method is described for the separation of oxytetracychne and its impurities. The separation is much better than that obtained with official pharmacopoeia methods. The method uses XTerra RP-18, 5 I~m (25 cm • 4.6 mm I.D.), a silica-based stationary phase with methyl end-capping, claimed to reduce silanol activity. The column temperature is set at 30 ~ and a UV detection is performed at 280 nm. Mobile phase containing acetonitrile -0.25 M tetrabutylammonium hydrogen sulfate pH Z5 -0.25 M ethylenediaminetetraacetic acid pH Z5 -water (115:360:160:365, v/v/v/v) is used at a flow rate of 1.0 mL.min 1, to separate the impurities present in oxytetracychne base.A central composite experimental design is used to optimize the separation. A second isocratic method with higher content of acetonitrile is needed to separate the more retained impurities present only in oxytetracychne hydrochloride. The method is robust and shows good selectivity, repeatability, hnearity and sensitivity.

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Section: Introductionmentioning

confidence: 99%

Development and validation of an improved liquid chromatographic method for the analysis of oxytetracycline

et al. 2002

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“…Further studies such as NMR, IR or LC/MS/MS might be needed to back up this supposition. On the other hand, OTC is known to be more stable toward epimerization, owing to the hydrogen bonding between C-5 hydroxyl and C-4 dimethylamino groups (Hussar et al, 1968;Khan et al, 1987). This is likely to be the reason why only a single, original peak was observed after encapsulation of OTC into the CTAB-water-ethyl formate reverse micelles.…”

Section: Resultsmentioning

confidence: 99%

“…For instance, OTC is the most commonly used veterinary drug in many food-producing animals. A number of studies have been carried out to evaluate the dependence of the chemical stability of TC upon pH, temperature and ionic strength (Regosz, 1977;Khan et al, 1987;Kühne et al, 2000;Wu and Fassihi, 2005). They demonstrated that TC possessed very low stability in aqueous media.…”

Section: Introductionmentioning

confidence: 99%

Degradation patterns of tetracycline antibiotics in reverse micelles and water

Sah

2006

Biomed. Chromatogr.

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The objective of this study was to determine the chemical stability of tetracycline and oxytetracycline hydro-chlorides in reverse micelles. Their reverse micellar solutions were prepared using cetyltrimethylammonium bromide, water and ethyl formate. The aqueous solutions of the tetracycline antibiotics were also prepared for comparison. The reverse micellar and aqueous solutions were stored at 37 degrees C. Samples were analyzed by high performance liquid chromatography. When evaluation was performed on an aqueous tetracycline HCl solution, its half-life was estimated to be 329 h. Its chemical stability was not improved after being dissolved in the reverse micelles, and a similar half-life of 330 h was observed. However, there were noticeable differences between the two systems in terms of degradation kinetics and degradation byproducts. On the other hand, oxytetracycline HCl was unstable in water so that its half-life was only 34 h. Very interestingly, pronounced improvement in stability was attained with the reverse micellar system: upon dissolving in the reverse micelles, its half-life was increased to 2402 h. There were also marked differences in degradation patterns and mechanisms of oxytetracycline HCl in water and the reverse micelles. Our study indicates that the reverse micellar system has potential applications in solubilizing and stabilizing oxytetracycline HCl, thereby contributing to the development of its dosage forms.

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“…They can form complexes with metal ions and strongly react with residual silanol groups on the HPLC column which often results in severe peak tailing [15,[34][35][36]. In order to avoid this problem, reverse phase-HPLC with mobile phases containing various acids (phosphoric, acetic, tartaric acids and EDTA) and also ion-pair chromatography were applied to sep- arate TCNs [37][38][39][40][41]. However, TCNs still showed extreme tailing on the RP-HPLC columns.…”

Section: Optimization Of Separation Conditionsmentioning

confidence: 99%

Carrier mediated hollow fiber liquid phase microextraction combined with HPLC–UV for preconcentration and determination of some tetracycline antibiotics

Shariati

Yamini

Esrafili

2009

Journal of Chromatography B

132

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Quantitative analysis of oxytetracycline and related substances by high-performance liquid chromatography

Cited by 50 publications

References 14 publications

Development and validation of an improved liquid chromatographic method for the analysis of oxytetracycline

Development and validation of an improved liquid chromatographic method for the analysis of oxytetracycline

Degradation patterns of tetracycline antibiotics in reverse micelles and water

Carrier mediated hollow fiber liquid phase microextraction combined with HPLC–UV for preconcentration and determination of some tetracycline antibiotics

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