Quantitative analysis of organophosphate insecticide metabolites in urine extracted from disposable diapers of toddlers in Japan

Oya, Naoko; Ito, Yuki; Hioki, Keisuke; Asai, Yuya; Aoi, Arisa; Sugiura, Yoshimi; Ueyama, Jun; Oguri, Tomoko; Kato, Sayaka; Ebara, Takeshi; Kamijima, Michihiro

doi:10.1016/j.ijheh.2016.10.009

Cited by 29 publications

(22 citation statements)

References 27 publications

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“…To further exemplify the advantages of the developed VA‐SI‐LLME/UHPLC/MS/MS method for the analysis of OPP metabolites from urine samples, the analytical features such as organic solvent volume, extraction time, LODs, and sample volume utilized in the developed method were compared with those of other reported methods for the tdetection of these metabolites . The comparison results are presented in Table .…”

Section: Resultsmentioning

confidence: 99%

“…14 urine samples, the analytical features such as organic solvent volume, extraction time, LODs, and sample volume utilized in the developed method were compared with those of other reported methods for the tdetection of these metabolites. 14,[16][17][18][19][20][21]23 The comparison results are presented in Table 3. The organic solvent consumption of the developed method is much less (200 μL) than for other reported methods, as mentioned in Table 3.…”

Section: Real Sample Analysismentioning

confidence: 99%

“…Sample pretreatment of the biological sample is a significant step in any bioanalysis even though it is the most intricate feature. Various sample pretreatment extraction methods have been developed including liquid–liquid extraction (LLE), solid‐phase extraction (SPE), stir‐bar sorptive extraction (SBSE), liquid–liquid microextraction (LLME), and several others for the extraction of OPP metabolites . All these methods possess drawbacks: they can be tedious and laborious, with long extraction times, challenges in sorbent selection and preparation for SPE, low sensitivity, and consumption of high volumes of organic solvents.…”

Section: Introductionmentioning

confidence: 99%

“…Various sample pretreatment extraction methods have been developed including liquid-liquid extraction (LLE), solid-phase extraction (SPE), 20,21 stir-bar sorptive extraction (SBSE), 22 liquid-liquid microextraction (LLME), and several others [23][24][25] for the extraction of OPP metabolites. [20][21][22][23][24][25] All these methods possess drawbacks: they can be tedious and laborious, with long extraction times, challenges in sorbent selection and preparation for SPE, low sensitivity, and consumption of high volumes of organic solvents. This, therefore, creates a need for the development of a facile, rapid, efficient, and eco-friendly sample preparation method for the determination of the metabolites of OPPs in biological samples.…”

Section: Introductionmentioning

confidence: 99%

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Rapid and sensitive analytical procedure for biomonitoring of organophosphate pesticide metabolites in human urine samples using a vortex‐assisted salt‐induced liquid–liquid microextraction technique coupled with ultra‐high‐performance liquid chromatography/tandem mass spectrometry

Pasupuleti

Tsai

Lin

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale Organophosphate pesticides (OPPs) are the most commonly used insecticides around the world in various agricultural and domestic practices, and humans are frequently exposed to these hazardous insecticides that can lead to several chronic health effects. Therefore, a fast and sensitive analytical method is required for biomonitoring the markers of OPPs in humans for exposure estimation. In this study, a fast and sensitive analytical procedure was developed for the determination of the metabolites of OPPs in human urine samples. Methods Metabolites of OPPs were extracted from 2 mL of urine sample using a novel vortex‐assisted salt‐induced liquid–liquid microextraction (VA‐SI‐LLME) technique, and the preconcentrated metabolites were analyzed by ultra‐high‐performance liquid chromatography/tandem mass spectrometry (UHPLC/MS/MS). Various factors affecting the efficiency of VA‐SI‐LLME were thoroughly investigated. Results The metabolites of OPPs exhibited very good linearity over the concentration range between 0.05 and 50 ng mL−1 with coefficient (r2) values ranging between 0.9986 and 0.9999. The method showed excellent sensitivity with detection limits ranging from 0.01 to 0.03 ng mL−1 and quantification limits from 0.03 to 0.05 ng mL−1. The developed method was applied to the analysis of real samples and the recoveries ranged between 85.0 and 114.1% with related standard deviations <5%. Conclusions The results showed the VA‐SI‐LLME/UHPLC/MS/MS method to be a simple, rapid, sensitive, and selective analytical procedure for the biomonitoring of the metabolites of OPPs in humans. This efficient and cost‐effective analytical method could be a potential alternative method for the biomonitoring of the metabolites of pesticides in humans.

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Section: Resultsmentioning

confidence: 99%

Section: Real Sample Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rapid and sensitive analytical procedure for biomonitoring of organophosphate pesticide metabolites in human urine samples using a vortex‐assisted salt‐induced liquid–liquid microextraction technique coupled with ultra‐high‐performance liquid chromatography/tandem mass spectrometry

Pasupuleti

Tsai

Lin

et al. 2020

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

show abstract

“…ours and they concluded that the brand could affect the extraction efficacy (Hu et al, 2004). Oya et al (2017) reported recoveries for organophosphate metabolites from a diaper ranging from 54% to 101% for dimethyl dithiophosphate and diethyl phosphate, respectively (Oya et al, 2017). The recoveries of pyrethroid metabolites reported by Hu et al (2004) were between 44% and 122%, and recovery data from Saito et al (2014) were between 10% and 123%.…”

Section: Discussionmentioning

confidence: 98%

Urine Collection Methods for Non-Toilet-Trained Children in Biological Monitoring Studies: Validation of a Disposable Diaper for Characterization of Tebuconazole Exposure

Oerlemans¹,

Dael²,

Vermeulen³

et al. 2018

ISEE Conference Abstracts

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Young children differ from adults in their exposure and susceptibility to environmental chemicals (e.g. pesticides) because of various factors such as behavior, diet and physiology. Their heightened vulnerability to environmental stressors makes it important to obtain appropriate urine samples for exposure characterization. However, collecting urine from non-toilet-trained children has been shown to be methodologically and practically challenging. Four urine collection approaches were tested: a disposable diaper, a urine bag, a collection pad and the clean catch. The success rate and the user rating of each method was evaluated. The success rates were 67%, 21%, 17% and 4% for the disposable diaper, urine bag, collection pad and clean catch, respectively. The average user ratings on a 0-10 (0 = inconvenient, 10 = convenient) scale were 9.0, 4.7, 7.3 and 2.5, respectively. Subsequently, the best rated method, the disposable polyacrylate diaper was tested with hydroxy-tebuconazole as an exposure biomarker for the fungicide tebuconazole and creatinine for urine density adjustment. After LC-MS/MS analysis, the recoveries of hydroxy-tebuconazole in the range of 0.05-25 ng/mL were on average 106%, and for creatinine 87%. Precisions (relative standard deviation) were for both 3%. The overall procedure including collection and extraction was assessed, resulting in three out of seven positive samples. Based on this study, the disposable diaper is a suitable method for urine collection of non-toilet-trained children for biomonitoring of tebuconazole. This method can serve as a basis for extension to other substances of interest.

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Environmental Exposure Measurements and Evaluations

Xia

Wang

Chen

2020

Early-Life Environmental Exposure and Disease

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Quantitative analysis of organophosphate insecticide metabolites in urine extracted from disposable diapers of toddlers in Japan

Cited by 29 publications

References 27 publications

Rapid and sensitive analytical procedure for biomonitoring of organophosphate pesticide metabolites in human urine samples using a vortex‐assisted salt‐induced liquid–liquid microextraction technique coupled with ultra‐high‐performance liquid chromatography/tandem mass spectrometry

Rapid and sensitive analytical procedure for biomonitoring of organophosphate pesticide metabolites in human urine samples using a vortex‐assisted salt‐induced liquid–liquid microextraction technique coupled with ultra‐high‐performance liquid chromatography/tandem mass spectrometry

Urine Collection Methods for Non-Toilet-Trained Children in Biological Monitoring Studies: Validation of a Disposable Diaper for Characterization of Tebuconazole Exposure

Environmental Exposure Measurements and Evaluations

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