Quantitative analysis of ochratoxin A in wine and beer using solid phase extraction and high performance liquid chromatography–fluorescence detection

Abstract

A reliable and accurate method is described for the quantitative analysis of ochratoxin A (OTA) in wine and beer. The method involves the use of disposable non-polar polymeric and aminopropyl solid-phase extraction cartridges to isolate the mycotoxin from alcoholic beverages. Extracts were subsequently analysed using reverse-phase high-performance liquid chromatography-fluorescence detection with post column ammoniation to improve the limit of detection. The precision of the method determined at three levels in both wine and beer was less than 5% (RSD). Standard addition studies in both wine and beer showed that the recovery of OTA varied between 90 and 106% over a concentration range of 0.016-1.284 microg l-1. The detection and quantification limits were shown to be better than 0.004 (S/N = 3) and 0.016 microg l-1 (S/N = 10) for both beer and wine.

Encapsulation of quantum dots and carbon nanotubes with polypyrrole in a syringe needle for automated molecularly imprinted solid phase pre-concentration of ochratoxin A in red wine analysis

“…As shown in Fig. 8b, the large peaks from different components in red [30]. In our work, MIPPy/CNTs and MIPPy/CNTs/QDs modified stainless steel needles were employed to preconcentrate OTA from red wine matrix, thereby cleaning up the sample.…”

Determination of Mycotoxins in Food Matrices Using LC-MS/MS Compared With High-resolution Orbitrap™ MS Technology

“…Three different separation modes can be used: normal phase (unmodified silica), ion exchange (strong or weak anion or cation exchangers bound to a silica support material) and reversedphase (in most cases C18 material) [43][44][45][46][47]. Additionally, special column material such as immunoaffinity material can be used.…”

Non-instrumental immunochemical tests for rapid ochratoxin A detection in red wine

“…Native fluorescence of OTA allows detection of OTA directly, or after OTA derivatisation [5][6][7][8][9]. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is also used for OTA detection in wine [3,10].…”

“…For OTA detection in wine an IAC-HPLC-FLD method [13] (EN 14133/AOAC 2001.01) was adopted as standard by the Association of Official Analytical Chemists International (AOAC), the European Committee for Standardisation (CEN) and the Organization Internationale de la Vigne et du Vin (OIV). As alternative for IAC application, several variants of solid-phase extraction [6,7,14] were developed. However, chromatographic methods are laborious, time-consuming, require sophisticated equipment and/or trained personnel, organic solvents and cannot be used on-site.…”