Quantitative analysis of mitragynine in human urine by high performance liquid chromatography-tandem mass spectrometry

Lu, Shijun; Tran, Buu N.; Nelsen, Jamie; Aldous, Kenneth M.

doi:10.1016/j.jchromb.2009.06.024

Cited by 53 publications

(28 citation statements)

References 18 publications

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“…, m/z 353), an alkaloid from Catharanthus roseus roots, shows two major fragments, which both result from cleavages in the piperidine ring (see Scheme 7; Lu et al, 2009b;Ferreres et al, 2010). Additionally, both [M þ H] þ and the fragment ion with m/z 210 show a loss of 32 Da (CH 3 OH) from the methyl ester group.…”

Section: Vasodilatorsmentioning

confidence: 99%

Fragmentation of toxicologically relevant drugs in positive‐ion liquid chromatography–tandem mass spectrometry

Niessen¹

2011

Mass Spectrometry Reviews

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The identification of drugs and related compounds by LC-MS-MS is an important analytical challenge in several application areas, including clinical and forensic toxicology, doping control analysis, and environmental analysis. Although target-compound based analytical strategies are most frequently applied, at some point the information content of the MS-MS spectra becomes relevant. In this article, the positive-ion MS-MS spectra of a wide variety of drugs and related substances are discussed. Starting point was an MS-MS mass spectral library of toxicologically relevant compounds, available on the internet. The positive-ion MS-MS spectra of ∼570 compounds were interpreted by chemical and therapeutic class, thus involving a wide variety of drug compound classes, such benzodiazepines, beta-blockers, angiotensin-converting enzyme inhibitors, phenothiazines, dihydropyridine calcium channel blockers, diuretics, local anesthetics, vasodilators, as well as various subclasses of anti-diabetic, antidepressant, analgesic, and antihistaminic drugs. In addition, the scientific literature was searched for available MS-MS data of these compound classes and the interpretation thereof. The results of this elaborate study are presented in this article. For each individual compound class, the emphasis is on class-specific fragmentation, as discussing fragmentation of all individual compounds would take far too much space. The recognition of class-specific fragmentation may be quite informative in determining the compound class of a specific unknown, which may further help in the identification. In addition, knowledge on (class-specific) fragmentation may further help in the optimization of the selectivity in targeted analytical approaches of compounds of one particular class.

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Section: Vasodilatorsmentioning

confidence: 99%

Fragmentation of toxicologically relevant drugs in positive‐ion liquid chromatography–tandem mass spectrometry

Niessen¹

2011

Mass Spectrometry Reviews

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“…Another reason for its popularity is the wide-spread use of MS coupled to HPLC, as HILIC mobile phases are well compatible and give high sensitivity [6]. Recently, HILIC has become very popular in bio-analytical applications because drugs and their metabolites are often polar structures [105][106][107][108][109][110].…”

Section: Hydrophilic Interaction Liquid Chromatography (Hilic)mentioning

confidence: 99%

A review of current trends and advances in modern bio-analytical methods: Chromatography and sample preparation

Nováková

Vlčková

2009

Analytica Chimica Acta

499

231

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“…Mitragyinine -the primary active alkaloid extracted from the leaves of Mitragyna speciosa Korth -was determined on silica HILIC column by use of methanol and aqueous ammonium acetate as eluent [63]. Mitragynine was extracted by methyl t-butyl ether and separated on a HILIC column.…”

Section: Alkaloidsmentioning

confidence: 99%

Application of hydrophilic interaction chromatography in phytochemical analysis

Petruczynik¹,

Waksmundzka‐Hajnos²

2013

Acta Chromatographica

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Summary. Hydrophilic interaction chromatography (HILIC) is a liquid chromatographytechnique when a polar stationary phases -silica or polar bonded phases and aqueous mobile phase containing amount of a less polar solvent (often acetonitrile) is applied. HILIC is important for the separation of highly polar substances including biologically active compounds, such as drugs, amino acids, peptides, proteins, nucleosides, neurotransmitters, etc. HILIC is also the appropriate method for analysis of plant extract polar components such as carbohydrates, amino acids, peptides, phenolic acids, flavonoids and some alkaloids. Plant extracts are usually multicomponent mixtures of compounds of wide polarity range, which often cannot be analyzed by use of a single separation and detection method because of the high chemical diversity. Good results are obtained by use of coupled method, e.g., reversed-phase liquid chromatography (RP-LC) and HILIC mode separation. The elution order in HILIC is usually opposite to that in RP separations. This orthogonality determines one of the advantages of HILIC -the possibility of creating multidimensional separations. An important feature of HILIC is the improved sensitivity with electrospray mass spectrometry. This is significant for the analysis of components existing in small concentration in multicomponent mixtures. The high acetonitrile content also gives additional advantage of HILIC -faster separations due to the lower viscosity of HILIC eluents compared to standard RP ones. The presented review deals with the optimization of HILIC separations and application of the method for analysis of plant extracts components.

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Quantitative analysis of mitragynine in human urine by high performance liquid chromatography-tandem mass spectrometry

Cited by 53 publications

References 18 publications

Fragmentation of toxicologically relevant drugs in positive‐ion liquid chromatography–tandem mass spectrometry

Fragmentation of toxicologically relevant drugs in positive‐ion liquid chromatography–tandem mass spectrometry

A review of current trends and advances in modern bio-analytical methods: Chromatography and sample preparation

Application of hydrophilic interaction chromatography in phytochemical analysis

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