Quantitative analysis of microbiota in saliva, supragingival, and subgingival plaque of Chinese adults with chronic periodontitis

He, Junyu; Huang, Wenjuan; Pan, Zhiwen; Cui, Hushan; Qi, Guilin; Zhou, Xueping; Chen, Hui

doi:10.1007/s00784-011-0654-4

Cited by 82 publications

(87 citation statements)

References 49 publications

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“…gingivalis , in SL were associated with that in SUBP by using a PCR detection-based method [21]. It was also confirmed by later studies using more sophisticated methods, such as quantitative PCR [22] and microarray [23]. Our current results derived by a 16S rRNA gene sequencing approach using a next-generation sequencer contributed further evidence that the analysis of SL provides precise information on the relative difference in abundance and shift of SL.…”

Section: Discussionsupporting

confidence: 78%

Relative abundance of total subgingival plaque-specific bacteria in salivary microbiota reflects the overall periodontal condition in patients with periodontitis

et al. 2017

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Increasing attention is being focused on evaluating the salivary microbiota as a promising method for monitoring oral health; however, its bacterial composition greatly differs from that of dental plaque microbiota, which is a dominant etiologic factor of oral diseases. This study evaluated the relative abundance of subgingival plaque-specific bacteria in the salivary microbiota and examined a relationship between the abundance and severity of periodontal condition in patients with periodontitis. Four samples (subgingival and supragingival plaques, saliva, and tongue coating) per each subject were collected from 14 patients with a broad range of severity of periodontitis before periodontal therapy. The bacterial composition was analyzed by 16S rRNA gene amplicon sequencing using Ion PGM. Of the 66 species-level operational taxonomic units (OTUs) representing the mean relative abundance of ≥ 1% in any of the four niches, 12 OTUs corresponding to known periodontal pathogens, including Porphyromonas gingivalis, were characteristically predominant in the subgingival plaque and constituted 37.3 ± 22.9% of the microbiota. The total relative abundance of these OTUs occupied only 1.6 ± 1.2% of the salivary microbiota, but significantly correlated with the percentage of diseased sites (periodontal pocket depth ≥ 4 mm; r = 0.78, P < 0.001), in addition to the abundance of subgingival plaque microbiota (r = 0.61, P = 0.02). After periodontal therapy, the total relative abundance of these 12 OTUs was evaluated as well as before periodontal therapy and reductions of the abundance through periodontal therapy were strongly correlated in saliva and subgingival plaque (r = 0.81, P < 0.001). Based on these results, salivary microbiota might be a promising target for the evaluation of subgingival plaque-derived bacteria representing the present condition of periodontal health.

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Section: Discussionsupporting

confidence: 78%

Relative abundance of total subgingival plaque-specific bacteria in salivary microbiota reflects the overall periodontal condition in patients with periodontitis

et al. 2017

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“…It also contributes to chronic periodontitis. Studies in Chinese, Korean, and southern Thai populations have shown high detection frequencies of A. actinomycetemcomitans ranging approximately from 40% to 75% of the sampling sites [29-32]. In contrast, a study in a Japanese population reported that less than 10% of the chronic periodontitis diseased site was positive for A. actinomycetemcomitans [33].…”

Section: Discussionmentioning

confidence: 99%

High incidence of Aggregatibacter actinomycetemcomitansinfection in patients with cerebral infarction and diabetic renal failure: a cross-sectional study

et al. 2013

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BackgroundRecent epidemiological studies suggest that periodontitis is a major risk factor for renal failure and cerebral infarction. The aim of this study was to evaluate the association among periodontitis, renal failure, and cerebral infarction, focusing on microbiological and immunological features.MethodsTwenty-one patients treated with hemodialysis (HD) were enrolled in this study. They were 8 with diabetic nephropathy and 13 with non-diabetic nephropathy. Blood examination, periodontal examination, brain magnetic resonance image (MRI), and dental radiography were performed on all patients. Subgingival plaque, saliva, and blood samples were analyzed for the periodontal pathogens, Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans), Porphyromonas gingivalis (P. gingivalis), and Prevotella intermedia (P. intermedia) using quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA).ResultsWe found that the patients with diabetic nephropathy had more A. actinomycetemcomitans compared with non-diabetic nephropathy (P = 0.038) in dental plaque. Furthermore, the patients with diabetic nephropathy showed a significantly higher incidence of cerebral infarction compared with those with non-diabetic nephropathy (P = 0.029). Clinical oral and radiographic scores tended to be higher among patients in the diabetic nephropathy group than in the non-diabetic nephropathy group.ConclusionsPeriodontal pathogens, particularly A. actinomycetemcomitans, may play a role, at least a part, in the development of cerebral infarction in Japanese HD patients with diabetic nephropathy.

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“…In the supragingival biofilm samples, P. intermedia, P. nigrescens and P. tannerae were found in 54%, 23.81%, and 64% of the samples, respectively. He et al 18 evaluated matched samples from saliva and supragingival and biofilms in Chinese adults without periodontal disease and observed that G1 (n = 15) G2 (n = 12) G3 (n = 15) G1 (n = 15) G2 (n = 12) G3 (n = 15) G1 (n = 15) G2 (n = 12) G3 (n = 15) 83.3% and 70.8% of the saliva and supragingival samples, respectively, harbored P. intermedia. The presence or absence of an infection in the root canal system did not affect the detection of some species in saliva or supragingival biofilm.…”

Section: Discussionmentioning

confidence: 99%

Saliva, supragingival biofilm and root canals can harbor gene associated with resistance to lactamic agents

et al. 2015

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This study aimed to determine the presence of Prevotella strains and genes associated with resistance to lactamics in different oral niches from patients with/without primary endodontic infections. Saliva (S) and supragingival biofilm (SB) were collected from three patient groups: Group I -no endodontic infection (n = 15); Group II -acute endodontic infection (n = 12); and Group III -chronic endodontic infection (n = 15). Root canal (RC) samples were collected from Groups II and III. The presence of P. intermedia, P nigrescens, P. tannerae and cfxA/cfxA2 gene was assessed by PCR. The cfxA/cfxA2 gene was not detected in all environments within the same patient. The cfxA/cfxA2 gene was present in 23.81% of S samples, 28.57% of SB samples, and 7.41% of RC samples. Prevotella species were detected in 53.97%, 47.62% and 34.56% of the S, SB, and RC samples, respectively. P. intermedia had a high frequency in saliva samples from Group 3. Saliva samples from Group 1 had higher detection rates of P. nigrescens than did Groups 2 and 3. Patients without endodontic disease had high frequencies of P. nigrescens in the SB samples. The presence or absence of spontaneous symptoms was not related to the detection rates for resistance genes in the RC samples. Saliva, supragingival biofilm and root canals can harbor resistant bacteria. The presence of symptomatology did not increase the presence of the cfxA/cfxA2 gene in the supragingival biofilm and inside root canals.

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Quantitative analysis of microbiota in saliva, supragingival, and subgingival plaque of Chinese adults with chronic periodontitis

Abstract: Examination of bacterial detection frequency and loads in Chinese adults may assist microbial studies of periodontal disease and will shed light on periodontal disease diagnosis and treatment using antibiotics in the Chinese population.

Cited by 82 publications

References 49 publications

Relative abundance of total subgingival plaque-specific bacteria in salivary microbiota reflects the overall periodontal condition in patients with periodontitis

Relative abundance of total subgingival plaque-specific bacteria in salivary microbiota reflects the overall periodontal condition in patients with periodontitis

High incidence of Aggregatibacter actinomycetemcomitansinfection in patients with cerebral infarction and diabetic renal failure: a cross-sectional study

Saliva, supragingival biofilm and root canals can harbor gene associated with resistance to lactamic agents

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