Quantitative Analysis of Matrine in Liquid Crystalline Nanoparticles by HPLC

Abstract

A reversed-phase high-performance liquid chromatographic method has been developed to quantitatively determine matrine in liquid crystal nanoparticles. The chromatographic method is carried out using an isocratic system. The mobile phase was composed of methanol-PBS(pH6.8)-triethylamine (50 : 50 : 0.1%) with a flow rate of 1 mL/min with SPD-20A UV/vis detector and the detection wavelength was at 220 nm. The linearity of matrine is in the range of 1.6 to 200.0 μg/mL. The regression equation is y = 10706x − 2959 (R 2 = 1.0). The average recovery is 101.7%; RSD = 2.22% (n = 9). This method provides a simple and accurate strategy to determine matrine in liquid crystalline nanoparticle.

The synergistic effects of rosehip oil and matrine against Icerya aegyptiaca (Douglas) (Hemiptera: Coccoidea) and the underlying mechanisms

“…Finally, the sample was filtered through a 0.22‐μm nylon syringe filter before an HPLC system (Shimadzu LC‐20A) equipped with a variable wavelength ultraviolet–visible detector was used to analyze the matrine content. The sample was analyzed using a method described by Peng et al 29 . with improved HPLC analysis and detection conditions.…”

“…Finally, the sample was filtered through a 0.22-μm nylon syringe filter before an HPLC system (Shimadzu LC-20A) equipped with a variable wavelength ultraviolet-visible detector was used to analyze the matrine content. The sample was analyzed using a method described by Peng et al 29 with improved HPLC analysis and detection conditions. The HPLC conditions were as follows: Eclipse XDB-C 18 column (150 mm × 4.6 mm, 5 μm); acetonitrile/PBS (0.2 mmol) (20:80, v/v) as the mobile phase; 1.0 ml min −1 flow rate; 30°C column temperature; 210 nm detection wavelength; and 20 μl injection volume.…”

Matrine@chitosan-D-proline nanocapsules as antifouling agents with antibacterial properties and biofilm dispersibility in the marine environment

“…The Mat@CS-Pro nanocapsule suspension (2 mg/ml) was placed in a dialysis bag, immersed in pH 5, 6, 7, and 8 PBS solutions, respectively, for 12 h. The size of the Mat@CS-Pro nanocapsules was detected by DLS and TEM after treatment in different pH PBS solutions. The dialysis solution was measured using UV-Vis, and the peak at about 220 nm represented matrine (Peng et al, 2014).…”

Methotrexate-Lactoferrin Targeted Exemestane Cubosomes for Synergistic Breast Cancer Therapy

“…They also have prolonged release profile owing to their very ordered and organized internal structures. LCNPs show better structural and storage stability at room temperature and lesser drug leakage than liposomes and they can also withstand thermal treatment ( Peng et al, 2014 ).…”