Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography–mass spectrometry

Pan, Xiangqing; Welti, Ruth; Wang, Xuemin

doi:10.1038/nprot.2010.37

Cited by 780 publications

(536 citation statements)

References 31 publications

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“…1D). The concentrations of JA and JA-Ile in WT seedlings (4.0 ± 0.6 ng g −1 FW and 0.22 ± 0.12 ng g −1 FW, respectively) were similar to those reported previously (Pan et al, 2010). Compared with WT seedlings, the aln-1 mutant showed a 2-fold increase in JA levels and a 6-fold increase in JA-Ile levels.…”

Section: The Aln Mutants Have Increased Levels Of Endogenous Ja and Jsupporting

confidence: 72%

Allantoin, a stress-related purine metabolite, can activate jasmonate signaling in a MYC2-regulated and abscisic acid-dependent manner

Ishiga

Watanabe

Konishi

et al. 2016

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Allantoin is a metabolic intermediate of purine catabolism that often accumulates in stressed plants. Recently, we used Arabidopsis knockout mutants (aln) of ALLANTOINASE to show that this purine metabolite activates abscisic acid (ABA) production, thereby stimulating stress-related gene expression and enhancing seedling tolerance to abiotic stress. A detailed re-examination of the microarray data of an aln mutant (aln-1) confirmed the increased expression of ABA-related genes and also revealed altered expression of genes involved in jasmonic acid (JA) responses, probably under the control of MYC2, a master switch in the JA signaling pathway. Consistent with the transcriptome profiles, the aln-1 mutant displayed increased JA levels and enhanced responses to mechanical wounding and exogenous JA. Moreover, aln mutants demonstrated modestly increased susceptibility to Pseudomonas syringae and Pectobacterium carotovorum, probably reflecting the antagonistic action of MYC2 on the defense against these bacterial phytopathogens. Exogenously administered allantoin elicited the expression of JA-responsive genes, including MYC2, in wild-type plants, supporting the idea that allantoin might be responsible for the observed JA-related phenotypes of aln mutants. However, mutants deficient in bioactive JA (jar1-1), insensitive to JA (myc2-3), or deficient in ABA (aba2-1 and bglu18) suppressed the effect of exogenous allantoin. The suppression was further confirmed in aln-1 jar1-1 and aln-1 bglu18 double mutants. These results indicate that allantoin can activate the MYC2-regulated JA signaling pathway through ABA production. Overall, this study suggests a possible connection of purine catabolism with stress hormone homeostasis and signaling, and highlights the potential importance of allantoin in these interactions.

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Section: The Aln Mutants Have Increased Levels Of Endogenous Ja and Jsupporting

confidence: 72%

Allantoin, a stress-related purine metabolite, can activate jasmonate signaling in a MYC2-regulated and abscisic acid-dependent manner

Ishiga

Watanabe

Konishi

et al. 2016

EXBOTJ

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“…Samples in diethyl ether were then dried and taken up in 150 mL of 5% (v/v) methanol, 94% (v/v) water, and 1% (v/v) acetic acid. A 20-mL aliquot was taken for HPLC (Agilent 1200 series with Phenomenex Kinetex 2.6 mm EVO C18 10034.6 mm column)-mass spectrometry (Sciex Q-Trap 4500) analysis according to Pan et al (2010). The retention time and identity of ABA were confirmed with commercial ABA samples.…”

Section: Measurements Of Leaf Aba Concentrationmentioning

confidence: 99%

Stomatal VPD Response: There Is More to the Story Than ABA

et al. 2017

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Guard cells shrink and close stomatal pores when air humidity decreases (i.e. when the difference between the vapor pressures of leaf and atmosphere [VPD] increases). The role of abscisic acid (ABA) in VPD-induced stomatal closure has been studied using ABA-related mutants that respond to VPD in some studies and not in others. The importance of ABA biosynthesis in guard cells versus vasculature for whole-plant stomatal regulation is unclear as well. Here, we show that Arabidopsis (Arabidopsis thaliana) lines carrying mutations in different steps of ABA biosynthesis as well as pea (Pisum sativum) wilty and tomato (Solanum lycopersicum) flacca ABA-deficient mutants had higher stomatal conductance compared with wild-type plants. To characterize the role of ABA production in different cells, we generated transgenic plants where ABA biosynthesis was rescued in guard cells or phloem companion cells of an ABA-deficient mutant. In both cases, the whole-plant stomatal conductance, stunted growth phenotype, and leaf ABA level were restored to wild-type values, pointing to the redundancy of ABA sources and to the effectiveness of leaf ABA transport. All ABA-deficient lines closed their stomata rapidly and extensively in response to high VPD, whereas plants with mutated protein kinase OST1 showed stunted VPD-induced responses. Another strongly ABAinsensitive mutant, defective in the six ABA PYR/RCAR receptors, responded to changes in VPD in both directions strongly and symmetrically, indicating that its VPD-induced closure could be passive hydraulic. We discuss that both the VPD-induced passive hydraulic stomatal closure and the stomatal VPD regulation of ABA-deficient mutants may be conditional on the initial pretreatment stomatal conductance.

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“…The extraction procedure was modified from a previously published protocol (Pan et al, 2010). The leaf tissues (;0.6 g fresh weight) were ground into powder under liquid nitrogen and transferred to a 50-mL screw-cap tube.…”

Section: Phytohormone Extractionmentioning

confidence: 99%

Quantitative Peptidomics Study Reveals That a Wound-Induced Peptide from PR-1 Regulates Immune Signaling in Tomato

et al. 2014

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Many important cell-to-cell communication events in multicellular organisms are mediated by peptides, but only a few peptides have been identified in plants. In an attempt to address the difficulties in identifying plant signaling peptides, we developed a novel peptidomics approach and used this approach to discover defense signaling peptides in plants. In addition to the canonical peptide systemin, several novel peptides were confidently identified in tomato (Solanum lycopersicum) and quantified to be induced by both wounding and methyl jasmonate (MeJA). A wounding or wounding plus MeJA-induced peptide derived from the pathogenesis-related protein 1 (PR-1) family was found to induce significant antipathogen and minor antiherbivore responses in tomato. This study highlights a role for PR-1 in immune signaling and suggests the potential application of plant endogenous peptides in efforts to defeat biological threats in crop production. As PR-1 is highly conserved across many organisms and the putative peptide from At-PR1 was also found to be bioactive in Arabidopsis thaliana, our results suggest that this peptide may be useful for enhancing resistance to stress in other plant species.

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Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography–mass spectrometry

Cited by 780 publications

References 31 publications

Allantoin, a stress-related purine metabolite, can activate jasmonate signaling in a MYC2-regulated and abscisic acid-dependent manner

Allantoin, a stress-related purine metabolite, can activate jasmonate signaling in a MYC2-regulated and abscisic acid-dependent manner

Stomatal VPD Response: There Is More to the Story Than ABA

Quantitative Peptidomics Study Reveals That a Wound-Induced Peptide from PR-1 Regulates Immune Signaling in Tomato

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