2018
DOI: 10.1158/2326-6066.cir-17-0360
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Quantitative Analysis of Immune Infiltrates in Primary Melanoma

Abstract: Novel methods to analyze the tumor microenvironment (TME) are urgently needed to stratify melanoma patients for adjuvant immunotherapy. Tumor-infiltrating lymphocyte (TIL) analysis, by conventional pathologic methods, is predictive but is insufficiently precise for clinical application. Quantitative multiplex immunofluorescence (qmIF) allows for evaluation of the TME using multiparameter phenotyping, tissue segmentation, and quantitative spatial analysis (qSA). Given that CD3CD8 cytotoxic lymphocytes (CTLs) pr… Show more

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Cited by 91 publications
(106 citation statements)
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References 53 publications
(84 reference statements)
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“…The immunoscore, consisting of a more precise quantification of CD8 þ T cells within the tumor microenvironment, has been proposed as a biomarker in multiple tumor types (34)(35)(36). Recently, we have found that the ratio of CD8 þ T cells to CD68 þ macrophages in the stroma confers a favorable prognosis in a single patient cohort of stage II-III melanoma patients (37). Histopathologic as well as genomic assessments of the tumor immune microenvironment are likely to provide useful prognostic information in early-stage melanoma, and a combined biomarker including a more quantitative assessment of TILs will likely have application in the clinical setting.…”
Section: Discussionmentioning
confidence: 99%
“…The immunoscore, consisting of a more precise quantification of CD8 þ T cells within the tumor microenvironment, has been proposed as a biomarker in multiple tumor types (34)(35)(36). Recently, we have found that the ratio of CD8 þ T cells to CD68 þ macrophages in the stroma confers a favorable prognosis in a single patient cohort of stage II-III melanoma patients (37). Histopathologic as well as genomic assessments of the tumor immune microenvironment are likely to provide useful prognostic information in early-stage melanoma, and a combined biomarker including a more quantitative assessment of TILs will likely have application in the clinical setting.…”
Section: Discussionmentioning
confidence: 99%
“…The TAMs can be originated from circulating monocytes that will enter the tissue and differentiate into macrophages, bone-marrow-derived macrophages (BMDMs) or can result from an accumulation of tissueresident macrophages (TRMs) (Pathria et al, 2019). Indeed, there is a crescent number of reports correlating TAMs with higher tumor grade and shorter survival for breast cancer, renal cell carcinoma, glioblastoma, pancreatic cancer, head and neck cancer, and lymphoma (Zhang et al, 2013(Zhang et al, , 2018Pedersen et al, 2014;Tiainen et al, 2015;Wang et al, 2015;Hu et al, 2016;Atanasov et al, 2018;Gartrell et al, 2018;Sorensen et al, 2018;Pathria et al, 2019). The relationship between TAMs and the tumor invasiveness and ability to metastasis is suggested to be related to epithelial-mesenchymal transition (EMT) (Su et al, 2014;Fu et al, 2015;Ravi et al, 2016).…”
Section: Macrophages and Dendritic Cellsmentioning
confidence: 99%
“…Importantly, TIPC performance was robust to the choice of immune cell types, detection methods and parameter settings. Besides CD3 + T-cell, using a more specific CD3 + CD8 + CD45RO + T-cell subset 21,22 and two innate immune cell types i.e. eosinophils and neutrophils, TIPC was still able to determine prognostic spatial subtypes.…”
Section: Moreover Tipc Was Able To Reveal Heterogeneous T-cell Respomentioning
confidence: 99%