Quantitative analysis of hydrocolloids in food systems by methanolysis coupled to reverse HPLC. Part 1. Gelling carrageenans

Quéméner, Bernard; Marot, Cécile; Mouillet, L.; Riz, V Da; Diris, J

doi:10.1016/s0268-005x(99)00042-9

Cited by 30 publications

(14 citation statements)

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“…Protein content was estimated using the Kjeldahl nitrogen method [ 53 ]. Neutral sugar and uronic acid composition was determined after methanolysis in MeOH-HCl and HPLC analysis using an Absorbosphere RP18 column (5 μm, 4 mm × 250 mm, Grace, Deerfield, IL, USA) according to Quemener et al [ 18 ]. Molecular weight of the ulvan was determined as described by Robic et al [ 29 ]: briefly, ulvan was injected on a high-performance size exclusion chromatography (HPSEC) system (Agilent 1100 Series HPLC Value System, Santa Clara, CA, USA) constituted of a 8 × 300 mm Shodex OHpak SB-806M HQ column (Showa Denko KK, Miniato, Japan) and a 6 × 50 mm Shodex OHpak SB-G guard column (Showa Denko KK, Miniato, Japan) eluted at 0.7 mL.min -1 with 50 mM NaNO 3 containing 0.02% NaN; on-line molar mass determinations were performed using a multi-angle laser light scattering (MALLS) detector (mini-Dawn Wyatt, Santa Barbara, CA, USA) operating at three angles: 41°, 90° and 138° and a differential refractometer (ERC 7517A; Erma Inc., Tokyo, Japan), and a UV detector at 280 nm (LDC ⁄ Milton Roy SpectroMonitor 3000; LDC S.A., Paris, France)).…”

Section: Methodsmentioning

confidence: 99%

“…Ulvan, the most important one, has been characterized with a variety of biological features [ 8 , 9 , 10 , 11 , 12 , 13 , 14 ]; among these, the immunomodulatory effect and the antiviral activity have focused the attention of scientists worldwide and marked a trend in drugs development. The main constituents reported in ulvans from several Ulvales species [ 15 , 16 , 17 , 18 , 19 ] are: Rhamnose (16.8%–45.0% dw), xylose (2.1%–12.0%), glucose (0.5%–6.4%), uronic acid (6.5%–19.0%), sulphate (16.0%–23.2%) and iduronic acid (1.1%–9.1%), rhamnose occurring mainly in the form of the aldobiouronic acid, 4- O -β- d -glucuronosyl- l -rhamnose.…”

Section: Introductionmentioning

confidence: 99%

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Sulphated Polysaccharides from Ulva clathrata and Cladosiphon okamuranus Seaweeds both Inhibit Viral Attachment/Entry and Cell-Cell Fusion, in NDV Infection

et al. 2015

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Sulphated polysaccharides (SP) extracted from seaweeds have antiviral properties and are much less cytotoxic than conventional drugs, but little is known about their mode of action. Combination antiviral chemotherapy may offer advantages over single agent therapy, increasing efficiency, potency and delaying the emergence of resistant virus. The paramyxoviridae family includes pathogens causing morbidity and mortality worldwide in humans and animals, such as the Newcastle Disease Virus (NDV) in poultry. This study aims at determining the antiviral activity and mechanism of action in vitro of an ulvan (SP from the green seaweed Ulva clathrata), and of its mixture with a fucoidan (SP from Cladosiphon okamuranus), against La Sota NDV strain. The ulvan antiviral activity was tested using syncytia formation, exhibiting an IC50 of 0.1 μg/mL; ulvan had a better anti cell-cell spread effect than that previously shown for fucoidan, and inhibited cell-cell fusion via a direct effect on the F0 protein, but did not show any virucidal effect. The mixture of ulvan and fucoidan showed a greater anti-spread effect than SPs alone, but ulvan antagonizes the effect of fucoidan on the viral attachment/entry. Both SPs may be promising antivirals against paramyxovirus infection but their mixture has no clear synergistic advantage.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sulphated Polysaccharides from Ulva clathrata and Cladosiphon okamuranus Seaweeds both Inhibit Viral Attachment/Entry and Cell-Cell Fusion, in NDV Infection

et al. 2015

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“…Neutral sugars and uronic acid composition in extracts were determined after methanolysis in MeOH-HCl and high-performance liquid chromatography (HPLC) analysis (Absorbosphere RP18, 5 μm, 4×250 mm; Quemener et al 2000). Chromatographic peaks were identified by comparison with reference sugars except for iduronic acid which was identified by comparison with iduronic acid-rich laboratory samples.…”

Section: Methodsmentioning

confidence: 99%

Determination of the chemical composition of ulvan, a cell wall polysaccharide from Ulva spp. (Ulvales, Chlorophyta) by FT-IR and chemometrics

et al. 2008

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The water-soluble cell wall polysaccharides from green seaweeds of Ulva spp. (Ulvales, Chlorophyta), referred to as ulvan, demonstrate composition-and structure-related functional properties. Mid-infrared spectroscopy combined with chemometric techniques was investigated as a means to rapidly predict the chemical composition of ulvan extracts. A calibration was realized with 41 ulvan extracts from two Ulva species. The variables studied included the constituent sugars (rhamnose, xylose, glucose, galactose, glucuronic acid, iduronic acid), protein, and sulfate contents. The correlation between Fourier transform infrared and chemical data was developed using partial least squares (PLS) regression with full cross-validation (leave one out). The coefficients of determination in cross-validation (R 2 CAL ) and the standard error in cross-validation were determined for each variable. The PLS model validation resulted in a coefficient of determination (R 2 VAL ) and a standard error in prediction. Good predictions were obtained for rhamnose (R 2 VAL = 0.9244), xylose (R 2 VAL =0.8758), glucuronic acid (R 2 VAL =0.9415), and sulfate (R 2 VAL =0.9218), which are the main ulvan constituents. However, minor components such as proteins, glucose, galactose, and iduronic acid were not correctly predicted. This study showed that mid-infrared spectroscopy combined with PLS regression is a reliable and fast method for the quantification of the main chemical constituents of ulvan extracts.

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“…Total uronic acid content was measured by the m ‐phenyl phenol colorimetric method (Thibault 1979) using glucuronic acid as standard. Neutral sugars and uronic acid composition in extracts were determined after methanolysis in MeOH‐HCl and HPLC analysis (Absorbosphere RP18, 5 μm, 4 × 250 mm, Grace, Deerfield, IL, USA; Quemener et al. 2000).…”

mentioning

confidence: 99%

SEASONAL VARIABILITY OF PHYSICOCHEMICAL AND RHEOLOGICAL PROPERTIES OF ULVAN IN TWO ULVA SPECIES (CHLOROPHYTA) FROM THE BRITTANY COAST¹

Robic

Sassi

Dion

et al. 2009

Journal of Phycology

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The seasonal variability in the extraction yield, physicochemical characteristics, and rheological properties of ulvan from two Ulva species contributing to Brittany "green tides" has been studied. These seaweeds were collected in the water column for Ulva armoricana Dion, de Reviers et Coat and on hard substrata for Ulva rotundata Bliding. The maximum ulvan extraction efficiency was not related to the maximum ulvan content in the seaweeds, but with the active growth period of the seaweeds. Ulvan chemical structure, macromolecular characteristics, and rheological properties were affected by both species and seasons. The proportion of high-molecular-weight ulvan was the major factor positively correlated with the gelling properties. Characteristics of ulvan from U. rotundata subjected to tides were more affected by seasons than ulvan from U. armoricana living in a more constant environment. These results point to several useful recommendations concerning Ulva sp. biomass collected with regard to ulvan characteristics and uses.

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Quantitative analysis of hydrocolloids in food systems by methanolysis coupled to reverse HPLC. Part 1. Gelling carrageenans

Cited by 30 publications

References 12 publications

Sulphated Polysaccharides from Ulva clathrata and Cladosiphon okamuranus Seaweeds both Inhibit Viral Attachment/Entry and Cell-Cell Fusion, in NDV Infection

Sulphated Polysaccharides from Ulva clathrata and Cladosiphon okamuranus Seaweeds both Inhibit Viral Attachment/Entry and Cell-Cell Fusion, in NDV Infection

Determination of the chemical composition of ulvan, a cell wall polysaccharide from Ulva spp. (Ulvales, Chlorophyta) by FT-IR and chemometrics

SEASONAL VARIABILITY OF PHYSICOCHEMICAL AND RHEOLOGICAL PROPERTIES OF ULVAN IN TWO ULVA SPECIES (CHLOROPHYTA) FROM THE BRITTANY COAST¹

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Quantitative analysis of hydrocolloids in food systems by methanolysis coupled to reverse HPLC. Part 1. Gelling carrageenans

Cited by 30 publications

References 12 publications

Sulphated Polysaccharides from Ulva clathrata and Cladosiphon okamuranus Seaweeds both Inhibit Viral Attachment/Entry and Cell-Cell Fusion, in NDV Infection

Sulphated Polysaccharides from Ulva clathrata and Cladosiphon okamuranus Seaweeds both Inhibit Viral Attachment/Entry and Cell-Cell Fusion, in NDV Infection

Determination of the chemical composition of ulvan, a cell wall polysaccharide from Ulva spp. (Ulvales, Chlorophyta) by FT-IR and chemometrics

SEASONAL VARIABILITY OF PHYSICOCHEMICAL AND RHEOLOGICAL PROPERTIES OF ULVAN IN TWO ULVA SPECIES (CHLOROPHYTA) FROM THE BRITTANY COAST1

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SEASONAL VARIABILITY OF PHYSICOCHEMICAL AND RHEOLOGICAL PROPERTIES OF ULVAN IN TWO ULVA SPECIES (CHLOROPHYTA) FROM THE BRITTANY COAST¹