2008
DOI: 10.1021/ac702072c
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Quantitative Analysis of HIV-1 Protease Inhibitors in Cell Lysates Using MALDI-FTICR Mass Spectrometry

Abstract: In this report we explore the use of MALDI-FTICR mass spectrometry for the quantitative analysis of five HIV-1 protease inhibitors in cell lysates. 2,5-Dihydroxybenzoic acid (DHB) was used as the matrix. From a quantitative perspective, DHB is usually a poor matrix due to its poor shot-to-shot and poor spot-to-spot reproducibilities. We found that the quantitative precisions improved significantly when DMSO (dimethylsulfoxide) was added to the matrix solution. For lopinavir and ritonavir, currently the most fr… Show more

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Cited by 19 publications
(16 citation statements)
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“…leads to an observed optimum affinity for K þ (van Kampen et al, 2008aKampen et al, , 2009a. The optimum affinity for K þ is indeed the case when 2,5-DHB is used as the matrix.…”
Section: A Protonation Versus Cationizationmentioning
confidence: 73%
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“…leads to an observed optimum affinity for K þ (van Kampen et al, 2008aKampen et al, , 2009a. The optimum affinity for K þ is indeed the case when 2,5-DHB is used as the matrix.…”
Section: A Protonation Versus Cationizationmentioning
confidence: 73%
“…Dwell time of the ions does not contribute significantly to the analysis time, although the detection time of ions can take second(s) per scan in Fourier-transform ion cyclotron resonance (FTICR) MS and Orbitrap MS. With a high repetition rate laser that fires at 1,000-2,000 Hz, an averaged mass spectrum of high quality can be obtained in a few seconds with MALDI-time-of-flight (TOF) MS or MALDI-triple quadrupole MS (Hatsis et al, 2003;McLean, Russell, & Russell, 2003;Moskovets et al, 2006;Rathore et al, 2008). For quantitative analysis of, for instance, HIV protease inhibitors, analysis times for a single spot on the target plate have been reported of $3 min for MALDI-FTICR MS (20 Hz laser), $30 sec for MALDI-TOF MS (50 Hz laser), and $5 sec for MALDI-triple quadrupole MS (1,000 Hz laser; van Kampen et al, 2006Kampen et al, , 2008aKampen et al, ,b, 2009b. Recently, an analysis time of 1.75 min for an entire 384-well target plate was reported for an assay to screen for small-molecule inhibitors of enzymes with MALDI-triple quadrupole MS (Rathore et al, 2008).…”
Section: A High-throughput Analysismentioning
confidence: 99%
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